ERASynBio: Technologies to Enable BioMolecular Origami

ERASynBio：实现生物分子折纸的技术

• 批准号: 1445112
• 负责人: Sriram Kosuri
• 金额: $ 45.85万
• 依托单位: University of California-Los Angeles
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-07-15 至 2017-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1445112&HistoricalAwards=false
• 关键词: ERASynBio; Technologies; Enable; BioMolecular; Origami

This project, funded by the Systems and Synthetic Biology Program in MCB and the Biotechnology, Biochemical and Biomass Engineering Program in CBET, is part of a larger ERASynBio funded collaborative. The team of investigators will develop the rules that govern the design of complex nanometer scale structures made from nucleic acids and polypeptides, and use those rules to create new biological molecules that have never been seen before in nature. They will also develop the tools that will enable the assembly of these new materials that could potentially be used to control many aspects of cell function, or create new materials that could be used as sensors or in biomanufacturing. Technical: Biological organisms are capable of producing chemicals, materials and molecular machines that far exceed our engineering capabilities. Underlying these abilities are the unique properties of proteins, exquisitely evolved for function, allowing precise positioning of atoms and chemistries. Designing novel proteins is difficult because of our still incomplete understanding of how proteins fold for a given primary amino-acid sequence. In this project, researchers will apply principles of synthetic biology to define and modularize building blocks that can be combined in rational ways to enable control of 3D positioning in designed macromolecular structure. Members of the consortium have advanced design and engineering principles for polypeptide- and DNA-based nanostructures and developed next-generation gene synthesis to facilitate high-throughput approaches. The team will build on these foundations to engineer bio-macromolecular assemblies with shapes and functions of unprecedented complexity. They will deliver an expanded toolbox of polypeptide building elements; rules, design principles and methods for constructing complex bionanostructures; and routes to nucleic acid/ polypeptide-hybrid platforms for the community of synthetic biology. The project will expand the limits of the designed polypeptide and nucleic acid/protein hybrid providing a platform to facilitate their use in a wide range of biomanufacturing applications.

该项目由 MCB 的系统和合成生物学项目以及 CBET 的生物技术、生物化学和生物质工程项目资助，是 ERASynBio 资助的更大合作项目的一部分。研究人员团队将制定控制由核酸和多肽制成的复杂纳米级结构设计的规则，并利用这些规则来创建自然界中以前从未见过的新生物分子。他们还将开发工具来组装这些新材料，这些新材料可能用于控制细胞功能的许多方面，或者创造可用作传感器或生物制造的新材料。技术：生物有机体能够生产远远超出我们工程能力的化学品、材料和分子机器。这些能力的基础是蛋白质的独特性质，其功能经过精心进化，可以精确定位原子和化学物质。设计新的蛋白质很困难，因为我们对给定一级氨基酸序列的蛋白质如何折叠的了解还不完全。在该项目中，研究人员将应用合成生物学原理来定义和模块化构建块，这些构建块可以以合理的方式组合，从而能够控制设计的大分子结构中的 3D 定位。该联盟的成员拥有基于多肽和 DNA 的纳米结构的先进设计和工程原理，并开发了下一代基因合成以促进高通量方法。该团队将在此基础上设计具有前所未有的复杂形状和功能的生物大分子组件。他们将提供一个扩展的多肽构建元件工具箱；构建复杂生物纳米结构的规则、设计原则和方法；以及合成生物学界的核酸/多肽混合平台的路线。该项目将扩大所设计的多肽和核酸/蛋白质杂合体的限制，提供一个平台，促进它们在广泛的生物制造应用中的使用。