EAGER: Leucine Aminopeptidase - Regulator of the Stress-Induced Stromal Proteome

EAGER：亮氨酸氨基肽酶 - 应激诱导基质蛋白质组的调节剂

• 批准号: 1450331
• 负责人: Linda Walling
• 金额: $ 12.61万
• 依托单位: University of California-Riverside
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-08-01 至 2017-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1450331&HistoricalAwards=false
• 关键词: EAGER; Leucine; Aminopeptidase; Regulator; Stress

Over 40% of the world's food supply is lost to damage caused by pathogens, pests and weeds. Therefore, novel strategies to enhance plant productivity and resistance to pests will significantly increase the security of the world's food supply. To achieve this goal, a deep understanding the mechanisms that control resistance to insects is needed. This project is at the cutting edge of plant-insect interactions and will reveal how chloroplasts communicate with nuclei to coordinate a robust defense after injury and insect attack; this knowledge will enable new strategies for insect resistance in the future. This project is a fertile training ground for a graduate student. Graduate student leadership skills will be developed through guiding undergraduate's in this project. UCR is a minority-serving institution and this project will recruit and engage students with diverse backgrounds. Results from the project will be disseminated by publications in scientific journals, lay talks to Riverside K-12 schools and the public, and talks at meetings and universities.This project will study the importance of a tomato chloroplast-localized protein called leucine aminopeptidase (LAP-A) and its role in generating a signal that controls nuclear gene expression after insect feeding and wounding. This project will identify the LAP-A-dependent signal that mediates this chloroplast-to-nucleus communication. The protein/peptide (target) that LAP-A acts on will be identified and the target may be the signal or may create the signal. LAP-A has two activities. LAP-A is an aminopeptidase and may produce the signal by modifying the N-terminus of a target protein/peptide that resides in the chloroplast. LAP-A is also a molecular chaperone and may help targets stay in an active folded state. These activities should influence the target's structure, activity and/or stability and thereby signal production. This project will use cutting-edge proteomics technologies to measure proteins/peptides in three tomato lines that express different levels of LAP-A (LapA-silenced, LapA-overexpressing, and wild type). Aim 1 will identify chloroplast proteins that are (1) cut by LAP-A and (2) have altered stability that is strictly correlated with the presence/absence of LAP-A. Aim 2 will determine the LAP-A-dependent changes in a small peptide called glutathione (GSH). Preliminary data link LAP-A and GSH turnover. GSH is an excellent candidate for the signal as it regulates two defense signals (reactive oxygen species and redox status) in other systems. The identity of LAP-A's targets will reveal the nature of the LAP-A-generated signal that mediates chloroplast-nucleus communication.

世界上超过40%的粮食供应因病原体、害虫和杂草造成的破坏而损失。因此，提高植物生产力和抗虫害能力的新策略将大大提高世界粮食供应的安全性。为了实现这一目标，需要深入了解控制昆虫抗性的机制。该项目处于植物与昆虫相互作用的前沿，将揭示叶绿体如何与细胞核沟通以协调受伤和昆虫攻击后的强大防御；这些知识将使未来的抗虫新策略成为可能。这个项目是培养研究生的沃土。通过对本科生的指导，培养研究生的领导能力。加州大学洛杉矶分校是一所为少数族裔服务的机构，该项目将招收和吸引来自不同背景的学生。该项目的成果将通过在科学期刊上的出版物，在河滨K-12学校和公众的演讲，以及在会议和大学的演讲来传播。该项目将研究番茄叶绿体定位蛋白亮氨酸氨基肽酶（LAP-A）的重要性及其在昆虫摄食和伤害后产生控制核基因表达的信号中的作用。该项目将确定介导叶绿体到细胞核通信的lap - a依赖信号。LAP-A作用的蛋白/肽（靶标）将被识别，靶标可能是信号或可能产生信号。LAP-A有两个活动。LAP-A是一种氨基肽酶，可以通过修饰叶绿体中靶蛋白/肽的n端来产生信号。LAP-A也是一种分子伴侣，可以帮助靶标保持活跃的折叠状态。这些活动应影响目标的结构、活性和/或稳定性，从而发出生产信号。该项目将使用尖端的蛋白质组学技术来测量三种番茄品系中表达不同水平的lapa （lapa沉默型、lapa过表达型和野生型）的蛋白质/肽。目的1将鉴定：(1)被LAP-A切割的叶绿体蛋白和(2)与LAP-A的存在/不存在严格相关的稳定性改变的叶绿体蛋白。目的2将确定一种叫做谷胱甘肽（GSH）的小肽的lap - a依赖性变化。初步数据链接LAP-A和谷胱甘肽周转。谷胱甘肽是一个很好的候选信号，因为它调节其他系统中的两种防御信号（活性氧和氧化还原状态）。LAP-A靶点的身份将揭示LAP-A产生的介导叶绿体-细胞核通讯的信号的性质。