Leucine Aminopeptidases: Role in Wounding and Defense
亮氨酸氨基肽酶:在损伤和防御中的作用
基本信息
- 批准号:0077862
- 负责人:
- 金额:$ 34.5万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Continuing Grant
- 财政年份:2000
- 资助国家:美国
- 起止时间:2000-09-01 至 2004-08-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Little is known about the degradation of proteins during plant development and in response to stress. In particular, there is a gap in the understanding of the class of proteases called aminopeptidases. Two classes of leucine aminopeptidase genes (LapA and LapN) from tomato have been characterized. LapA genes are expressed in response to wounding, caterpillar feeding, and some bacterial and fungal pathogens. In contrast, LapN genes are expressed at all times and in all tissues. The analysis of genetically engineered tomato plants that express a LapA antisense gene (35S:asLap) showed that down-regulation of Lap genes impairs the ability of tomatoes to mount a wound response. This proposal builds on a strong foundation of knowledge about the tomato LAPs built during the previous granting period. This proposed integrated research effort is focused on understanding the location of LAPs and their role in responses wounding, pathogens, water deficit, and salinity. Cell fractionation studies show that LAPs reside in two cellular compartments: the cytoplasm and the chloroplast. Obviously, the substrates available for LAP action are determined by the location of LAPs within the tomato cell. Therefore, LAP-A- and LAP-N-specific antibodies will be used to determine the location of these two classes of aminopeptidases within the cell. The mechanisms that control the accumulation of LAP-A and LAP-N in two cellular locations will be tested. The LapA antisense plants (35S:asLap) do not effectively induce wound-response genes. These data suggest that LAP-A may be one of the first peptidases to regulate a plant-signaling pathway. Since this conclusion is novel for the plant community, these data should be confirmed using additional strategies. Three sets of transgenic tomato plants that have altered patterns of Lap gene expression will be evaluated. First, plants expressing two Lap antisense genes will be made. These plants express the LapA antisense RNA from both the wound-induced LapA promoter (LapA:asLap) and the constitutive 35S promoter (35S:asLap). These plants should afford the tightest regulation of the wound response. Second, co-suppressed plants will be characterized. Unlike the antisense plants that down regulate both LapA and LapN, the co-suppressed plants only down regulate LapA. Third, transgenic tomatoes that express high levels of LAP-A constitutively will be characterized. A tight correlation of LapA and/or LapN down regulation with the suppression of the wound response will implicate LAPs as protease that regulates plant stress responses. If LAPs are shown to modulate the wound response, the site of LAP action will be determined by feeding Lap down-regulated plants systemin, abscisic acid, jasmonic acid and intermediates of the octadecanoid pathway. If systemin activation is implicated, the activity of LAP-A and LAP-N on putative systemin precursors will be characterized. Transgenic tomatoes that are up- and down-regulated for Lap gene expression will be used to determine if LAPs are important for controlling bacterial, viral, and fungal infections, as well as insect infestations and tolerance to water-deficit and salinity. The understanding of the expression and impact of LAPs in plant development and in responses to stress has increased greatly. However, continued efforts are essential to elucidate the exact roles of LAPs in stress responses. These studies will enhance our understanding of the mechanisms that favor optimal growth and development. These factors will directly impact on increased crop productivity and may yield significant insights for the development of cogent strategies to enhance a plant's endogenous protection mechanisms to an array of stresses.
对植物发育过程中蛋白质的降解以及对胁迫的反应知之甚少。特别是,有一个空白的理解一类蛋白酶称为氨肽酶。番茄中的两类亮氨酸氨肽酶基因(LapA和LapN)已被鉴定。LapA基因表达响应于创伤、毛虫进食以及一些细菌和真菌病原体。相反,LapN基因在所有时间和所有组织中表达。对表达LapA反义基因(35 S:asLap)的基因工程番茄植物的分析表明,Lap基因的下调损害了番茄产生伤口反应的能力。该提案建立在对前一个授予期内建立的番茄LAPs的强大知识基础之上。这项综合研究的重点是了解LAPs的位置及其在创伤,病原体,缺水和盐度反应中的作用。 细胞分级分离研究表明,LAPs存在于两个细胞隔室:细胞质和叶绿体。显然,可用于抑制作用的底物由LAPs在番茄细胞内的位置决定。因此,LAP-A和LAP-N特异性抗体将用于确定这两类氨肽酶在细胞内的位置。将测试控制LAP-A和LAP-N在两个细胞位置中蓄积的机制。LapA反义植物(35 S:asLap)不能有效地诱导伤口反应基因。这些数据表明LAP-A可能是调节植物信号传导途径的第一种肽酶之一。由于这一结论是新的植物群落,这些数据应确认使用其他策略。将评估三组具有改变的Lap基因表达模式的转基因番茄植物。首先,制备表达两个Lap反义基因的植物。这些植物表达来自创伤诱导的LapA启动子(LapA:asLap)和组成型35 S启动子(35 S:asLap)的LapA反义RNA。这些植物应该提供最严格的伤口反应调节。第二,将表征共抑制植物。与下调LapA和LapN的反义植物不同,共抑制的植物仅下调LapA。第三,将表征组成型表达高水平LAP-A的转基因番茄。LapA和/或LapN下调与伤口反应的抑制的紧密相关性将暗示LAP作为调节植物胁迫反应的蛋白酶。如果LAP显示调节伤口反应,则通过饲喂LAP下调的植物系统素、脱落酸、茉莉酸和十八烷酸途径的中间体来确定抑制作用的位点。如果涉及系统素活化,则将表征LAP-A和LAP-N对推定的系统素前体的活性。 Lap基因表达上调和下调的转基因番茄将用于确定Lap是否对控制细菌、病毒和真菌感染以及昆虫侵染和对缺水和盐度的耐受性很重要。 对LAPs在植物发育和胁迫响应中的表达和影响的理解大大增加。然而,持续的努力是必不可少的,以阐明在应激反应的LAPs的确切作用。这些研究将增强我们对有利于最佳生长和发育的机制的理解。这些因素将直接影响作物生产力的提高,并可能产生显着的见解,为发展有说服力的战略,以提高植物的内源性保护机制,以一系列的压力。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Linda Walling其他文献
BIOCHEMICAL AND MORPHOLOGIC OBSERVATIONS IN SEVERE INFANTILE FRUCTOSE INTOLERANCE
严重婴儿果糖不耐受的生化和形态学观察
- DOI:
10.1203/00006450-197404000-00543 - 发表时间:
1974-04-01 - 期刊:
- 影响因子:3.100
- 作者:
Philip Bagnell;George Hug;Linda Walling;William K Schubert - 通讯作者:
William K Schubert
Linda Walling的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Linda Walling', 18)}}的其他基金
EAGER: Leucine Aminopeptidase - Regulator of the Stress-Induced Stromal Proteome
EAGER:亮氨酸氨基肽酶 - 应激诱导基质蛋白质组的调节剂
- 批准号:
1450331 - 财政年份:2014
- 资助金额:
$ 34.5万 - 项目类别:
Standard Grant
Facing the Challenges of Vector-Borne Diseases in the 21st Century: Symposium; New Genomics Building at the University of California-Riverside
面对 21 世纪媒介传播疾病的挑战:研讨会;
- 批准号:
1033712 - 财政年份:2010
- 资助金额:
$ 34.5万 - 项目类别:
Standard Grant
Leucine Aminopeptidase: A Regulator of the Late Branch of Wound Signaling
亮氨酸氨肽酶:伤口信号传导晚期分支的调节剂
- 批准号:
0725093 - 财政年份:2007
- 资助金额:
$ 34.5万 - 项目类别:
Standard Grant
Arabidopsis 2010 Project: Collaborative Research: The Functional Genomics Approach to N-terminal Modifying Enzymes
拟南芥 2010 项目:合作研究:N 端修饰酶的功能基因组学方法
- 批准号:
0313579 - 财政年份:2003
- 资助金额:
$ 34.5万 - 项目类别:
Continuing Grant
The Role of Leucine Aminopeptidase in Plant Defense
亮氨酸氨肽酶在植物防御中的作用
- 批准号:
9318260 - 财政年份:1994
- 资助金额:
$ 34.5万 - 项目类别:
Continuing Grant
Characterization of a Cytoplasmically Inherited Mututation, Cyt-G1
细胞质遗传突变 Cyt-G1 的表征
- 批准号:
8910462 - 财政年份:1989
- 资助金额:
$ 34.5万 - 项目类别:
Standard Grant
相似海外基金
The role of post-proteolytic aminopeptidases in skeletal muscle homeostasis
蛋白水解后氨肽酶在骨骼肌稳态中的作用
- 批准号:
21H04857 - 财政年份:2021
- 资助金额:
$ 34.5万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
ER-aminopeptidases: Conformational regulation and antigen presentation function
ER-氨基肽酶:构象调节和抗原呈递功能
- 批准号:
10448371 - 财政年份:2020
- 资助金额:
$ 34.5万 - 项目类别:
Pharmacokinetics and pharmacodynamics of human recombinant aminopeptidases in model animals for hypertensive disorders of pregnancy
人重组氨肽酶在妊娠期高血压疾病模型动物中的药动学和药效学
- 批准号:
20K21475 - 财政年份:2020
- 资助金额:
$ 34.5万 - 项目类别:
Grant-in-Aid for Challenging Research (Exploratory)
ER-aminopeptidases: Conformational regulation and antigen presentation function
ER-氨基肽酶:构象调节和抗原呈递功能
- 批准号:
10664968 - 财政年份:2020
- 资助金额:
$ 34.5万 - 项目类别:
ER-aminopeptidases: Conformational regulation and antigen presentation function
ER-氨基肽酶:构象调节和抗原呈递功能
- 批准号:
10045425 - 财政年份:2020
- 资助金额:
$ 34.5万 - 项目类别:
Structural and Bioinformatics Analyses of M1 Aminopeptidases
M1 氨基肽酶的结构和生物信息学分析
- 批准号:
10437967 - 财政年份:2018
- 资助金额:
$ 34.5万 - 项目类别:
Structural and functional studies of dipeptidyl aminopeptidases from periodontal pathogen.
牙周病原体二肽基氨基肽酶的结构和功能研究。
- 批准号:
25462872 - 财政年份:2013
- 资助金额:
$ 34.5万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Aminopeptidases for Neurotoxic Pyroglutamate Beta-Amyloid of Alzheimers Disease
氨基肽酶治疗阿尔茨海默病的神经毒性焦谷氨酸β-淀粉样蛋白
- 批准号:
8583849 - 财政年份:2013
- 资助金额:
$ 34.5万 - 项目类别:
Pathophysiological roles of M1 aminopeptidases
M1 氨肽酶的病理生理学作用
- 批准号:
25293083 - 财政年份:2013
- 资助金额:
$ 34.5万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Aminopeptidases for Neurotoxic Pyroglutamate Beta-Amyloid of Alzheimers Disease
氨基肽酶治疗阿尔茨海默病的神经毒性焦谷氨酸β-淀粉样蛋白
- 批准号:
8690732 - 财政年份:2013
- 资助金额:
$ 34.5万 - 项目类别: