CAREER: Roles for Indole-3-butyric Acid in Plant Development

职业：3-吲哚丁酸在植物发育中的作用

• 批准号: 1453750
• 负责人: Lucia Strader
• 金额: $ 86.62万
• 依托单位: Washington University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-04-01 至 2021-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1453750&HistoricalAwards=false
• 关键词: CAREER; Roles; Indole; butyric; Acid

The plant hormone auxin regulates plant growth and development to control crop yield. Indole-3-butyric acid, or IBA, is an inactive auxin precursor whose transport throughout the plant and conversion to the active hormone is critical to auxin function. Despite the great importance of IBA to plant growth, the mechanisms controlling IBA input into the level of available auxin are not well understood. This proposal aims to increase our understanding of how IBA-derived auxin impacts plant growth; this understanding will provide new tools to alter plant growth and development for use in crop improvement. This CAREER proposal outlines experiments to elucidate developmental roles for indole-3-butyric acid (IBA) transport and IBA-derived auxin in the model plants Arabidopsis thaliana and Physcomitrella patens. Auxin is essential to plants, controlling cell division and cell expansion to orchestrate many developmental events and environmental responses. IBA is a biologically inactive molecule that is converted into the active auxin IAA (indole-3-acetic acid) by peroxisomal β-oxidation. IBA-derived auxin drives a wide range of seedling developmental processes, including cotyledon and root hair cell expansion, apical hook curvature, high-temperature-induced hypocotyl elongation, lateral root production, and root meristem maintenance. Similar to the active auxin IAA, the auxin precursor IBA is transported by carrier-mediated mechanisms.The proposed experiments focus on understanding roles for IBA transport and IBA-derived auxin in plant development with four specific aims: 1) To determine the tissue-specificity of IBA-to-IAA conversion. 2) To identify components of IBA transport. 3) To characterize TRANSPORTER OF IBA (TOB) family regulation of IBA transport and plant development. 4) To compare the roles of IBA-derived auxin and IBA transport in the nonvascular plant Physcomitrella patens. The proposed studies will advance our understanding of auxin regulation of plant development.This research will also support the creation of an inquiry-based laboratory to increase opportunities for undergraduate research at Washington U. This course addresses a critical need for a cell biology lab course in the curriculum; the primary objectives of this course are to increase student ability to form and test hypotheses and to raise student awareness of the diverse biological systems available for basic research. Aim 4 of this proposal will be directly integrated into the course and students will present their work at the WU Research Symposium at the end of the semester. In addition, this research will support the Missouri Local Auxin Meeting, co-organized by the PI to strengthen and expand the local auxin community, and additional K-12 outreach activities.

植物激素生长素调节植物生长和发育以控制作物产量。 吲哚-3-丁酸，或IBA，是一种非活性的生长素前体，其在整个植物中的运输和转化为活性激素对生长素功能至关重要。 尽管IBA对植物生长非常重要，但控制IBA输入到有效生长素水平的机制还不清楚。 该提案旨在增加我们对IBA衍生的生长素如何影响植物生长的理解;这种理解将提供新的工具来改变植物生长和发育，用于作物改良。 这个职业生涯的建议概述了实验，以阐明模式植物拟南芥和小立碗藓中吲哚-3-丁酸（IBA）的运输和IBA衍生的生长素的发育作用。生长素对植物至关重要，控制细胞分裂和细胞扩张以协调许多发育事件和环境反应。IBA是一种生物学上无活性的分子，通过过氧化物酶体的氧化作用可转化为活性生长素IAA（吲哚-3-乙酸）。 IBA衍生的生长素驱动广泛的幼苗发育过程，包括子叶和根毛细胞扩张、顶端钩弯曲、高温诱导的下胚轴伸长、侧根产生和根分生组织维持。 本实验旨在研究生长素前体IBA在植物生长发育过程中的作用，主要有以下四个方面：1）确定IBA转化为IAA的组织特异性; 2)确定IBA运输的组成部分。 3)研究IBA转运蛋白（TOB）家族对IBA转运和植物发育的调控。 4)比较小立碗藓中IBA衍生的生长素和IBA的运输作用。这项研究将促进我们对植物生长素调控植物发育的理解，也将支持建立一个以探究为基础的实验室，以增加华盛顿大学本科生的研究机会。本课程解决了课程中细胞生物学实验室课程的关键需求;本课程的主要目标是提高学生形成和测试假设的能力，并提高学生对基础研究可用的各种生物系统的认识。本提案的目标4将直接整合到课程中，学生将在学期末的WU研究研讨会上展示他们的工作。此外，这项研究将支持密苏里州当地生长素会议，由PI共同组织，以加强和扩大当地生长素社区，以及额外的K-12外展活动。