IDBR: TYPE A/Surface-sensitive coherent Raman microscopy (SCRM): label-free chemical imaging of dynamics at the cell surface

IDBR：A 型/表面敏感相干拉曼显微镜 (SCRM)：细胞表面动力学的无标记化学成像

• 批准号: 1454885
• 负责人: Eric Potma
• 金额: $ 32.91万
• 依托单位: University of California-Irvine
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-04-15 至 2018-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1454885&HistoricalAwards=false
• 关键词: IDBR; TYPE; Surface; sensitive; coherent

An award is made to the University of California, Irvine (UCI), to develop a new type of microscope that can be used to study the properties of cell membranes. The SCRM instrument provides a unique solution for cell membrane studies where the use of fluorescent labels is questionable, thus enabling new discoveries in cell biology and membrane biophysics not previously possible with current technologies. The development and application of the SCRM technology forms a thorough training opportunity for two graduate students and one undergraduate student in the areas of engineering, optical physics and biology. An important component of this program is to bring the new SCRM technology into the classroom, which is achieved by using the prototype instrument in a graduate level course on Molecular Biophysics. The new microscope will also be actively used in the Laboratory Experiments & Activities in the Physical Sciences (LEAPS) program, which brings underrepresented students from the Santa Ana School District to the UCI campus. The special feature of this microscope is its capability to interrogate membranes of live cells with high contrast, but without the use of fluorescent labels. This new mode of inquisition, called surface-sensitive coherent Raman microscopy (SCRM), is achieved through merging the imaging properties of two existing techniques: coherent Raman scattering (CRS) microscopy and total internal reflection illumination. Compared to conventional CRS microscopy, SCRM offers a ten times higher axial resolution at imaging speeds of more than 10 frames per second.

加州大学欧文分校（University of California, Irvine， UCI）因开发一种新型显微镜而获奖，该显微镜可用于研究细胞膜的特性。SCRM仪器为使用荧光标记有问题的细胞膜研究提供了独特的解决方案，从而使细胞生物学和膜生物物理学的新发现在现有技术下是不可能的。SCRM技术的开发和应用为工程、光学物理和生物学领域的两名研究生和一名本科生提供了全面的培训机会。该项目的一个重要组成部分是将新的SCRM技术带入课堂，这是通过在分子生物物理学研究生课程中使用原型仪器实现的。新的显微镜也将积极用于物理科学实验室实验和活动（LEAPS）项目，该项目将来自圣安娜学区的代表性不足的学生带到UCI校园。这台显微镜的特殊之处在于它能够以高对比度检查活细胞膜，而无需使用荧光标记。这种新的研究模式，被称为表面敏感相干拉曼显微镜（SCRM），是通过合并两种现有技术的成像特性实现的：相干拉曼散射（CRS）显微镜和全内反射照明。与传统的CRS显微镜相比，SCRM在超过每秒10帧的成像速度下提供了十倍高的轴向分辨率。