Basement membrane composition in the normal and diabetic retina

正常和糖尿病视网膜中的基底膜组成

• 批准号: 21909860
• 负责人: Professor Dr. Mats Paulsson
• 金额: --
• 依托单位: Institut für Biochemie II
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2006
• 资助国家: 德国
• 起止时间: 2005-12-31 至 2009-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/21909860?language=en
• 关键词: Basement; membrane; composition; normal; diabetic

Basement membranes are required for cellular adhesion, migration and differentiation. They also play a particular role in the delimiting of tissue boundaries and the formation of vascular and neural networks. Changes in the deposition of the extracellular matrix during diabetes mellitus are well established; however the molecular composition of the basement membranes in the normal and diabetic retina is only poorly described. Further, alterations in the repertoire of cellular receptors for basement membrane components and the effects these changes have upon the cells of the retina have not been thoroughly investigated. We will use a panel of antibodies against the main basement membrane proteins and their receptors to describe the composition of the retinal basement membranes in the normal mouse and in two mouse models of diabetes mellitus. Mice at various stages of the disease will be studied. Once candidate proteins with a changed expression profile have been identified they will be examined for their expression pattern in the diseased human eye. Finally, where available, mice carrying null mutations for these proteins will studied for the presence of any ocular phenotype and will be bred to the diabetic model strains and any modulation in the course of the pathology will be described. This will allow determining the significance in the basement membrane changes in the development of diabetic retinopathy.

基底膜是细胞粘附、迁移和分化所必需的。它们还在组织边界的划定以及血管和神经网络的形成中发挥特殊作用。糖尿病期间细胞外基质沉积的变化已得到充分证实;然而，正常和糖尿病视网膜中基底膜的分子组成仅得到很少描述。此外，基底膜成分的细胞受体库的改变以及这些变化对视网膜细胞的影响尚未得到彻底研究。我们将使用一组针对主要基底膜蛋白及其受体的抗体来描述正常小鼠和两种糖尿病小鼠模型中视网膜基底膜的组成。将对处于疾病不同阶段的小鼠进行研究。一旦已经鉴定出具有改变的表达谱的候选蛋白质，将检查它们在患病人眼中的表达模式。最后，在可用的情况下，将研究携带这些蛋白质的无效突变的小鼠是否存在任何眼部表型，并将其培育成糖尿病模型品系，并将描述病理过程中的任何调节。这将允许确定糖尿病视网膜病变发展中基底膜变化的重要性。