Multiplexing FRET-based Tension Sensors to Reveal Cell Adhesion Forces during Collective Cell Migration (B09)

多重基于 FRET 的张力传感器揭示集体细胞迁移过程中的细胞粘附力 (B09)

• 批准号: 219291843
• 金额: --
• 依托单位: Institut für Integrative Zellbiologie und Physiologie (IIZP)
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2012
• 资助国家: 德国
• 起止时间: 2011-12-31 至 2020-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/219291843?language=en
• 关键词: Multiplexing; FRET; based; Tension; Sensors

In this project, we will apply a set of single-molecule calibrated tension sensor modules, which were developed and calibrated in the previous funding periods, to generate Förster resonance energy transfer (FRET)-based probes that report piconewton-scale forces across central components of integrin- and cadherin based adhesion structures. By combining these tension sensors with a recently established multiplexing approach, we will simultaneously investigate the molecular mechanics of cell-matrix and cell-cell adhesion during collective cell migration.

在这个项目中，我们将应用一组单分子校准的张力传感器模块，这些模块是在以前的资助期间开发和校准的，以产生基于Förster共振能量转移（FRET）的探针，这些探针报告了基于整合素和钙粘蛋白的粘附结构的中心组件之间的皮牛顿级力。通过将这些张力传感器与最近建立的多路复用方法相结合，我们将同时研究集体细胞迁移过程中细胞-基质和细胞-细胞粘附的分子力学。