Multiplexing FRET-based Tension Sensors to Reveal Cell Adhesion Forces during Collective Cell Migration (B09)

在这个项目中，我们将应用一套单分子校准的张力传感器模块，这些模块是在之前的资助期开发和校准的，用于产生基于Förster共振能量转移(FRET)的探头，该探头报告整合素和钙粘附素粘连结构的中心组件之间的皮牛顿力。通过将这些张力传感器与最近建立的多路复用方法相结合，我们将同时研究细胞-基质和细胞-细胞集体迁移过程中细胞-细胞黏附的分子机制。

In this project, we will apply a set of single-molecule calibrated tension sensor modules, which were developed and calibrated in the previous funding periods, to generate Förster resonance energy transfer (FRET)-based probes that report piconewton-scale forces across central components of integrin- and cadherin based adhesion structures. By combining these tension sensors with a recently established multiplexing approach, we will simultaneously investigate the molecular mechanics of cell-matrix and cell-cell adhesion during collective cell migration.