Multiplexing FRET-based Tension Sensors to Reveal Cell Adhesion Forces during Collective Cell Migration (B09)

在这个项目中，我们将应用一组单分子校准张力传感器模块，这些模块是在之前的资助期开发和校准的，以生成Förster共振能量转移（FRET）为基础的探针，该探针可以报告跨整合素和钙粘蛋白黏附结构的中心组件的皮牛顿级力。通过将这些张力传感器与最近建立的多路复用方法相结合，我们将同时研究细胞集体迁移过程中细胞-基质和细胞-细胞粘附的分子力学。

In this project, we will apply a set of single-molecule calibrated tension sensor modules, which were developed and calibrated in the previous funding periods, to generate Förster resonance energy transfer (FRET)-based probes that report piconewton-scale forces across central components of integrin- and cadherin based adhesion structures. By combining these tension sensors with a recently established multiplexing approach, we will simultaneously investigate the molecular mechanics of cell-matrix and cell-cell adhesion during collective cell migration.