EAGER: Rapid Screening and Identification of Organisms which Hyper-Accumulate Metals

EAGER：快速筛选和鉴定超积累金属的生物体

• 批准号: 1500815
• 负责人: Soumya Srivastava
• 金额: $ 20万
• 依托单位: Regents of the University of Idaho
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-09-01 至 2019-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1500815&HistoricalAwards=false
• 关键词: EAGER; Rapid; Screening; Identification; Organisms

1500815SrivastavaUniversity of IdahoRare earth elements (REE) are considered critical materials because of their essential usage in a variety of business sectors and their potential for supply shortages and scarcity. Recovery of REE from waste streams and end-of-life products is an attractive technology, however separation of REE from these materials can be costly and involve toxic and hazardous chemicals. Microorganisms have the capacity to selectively accumulate and fractionate metals including REE but have not been deployed as a separation technology for REE. The PI proposes to develop a high risk high payoff platform technology based on microfluidic separation using dielectrophoresis (DEP). The proposed work is anticipated to transform the screening of biomaterials by providing a highly adaptable means for high-throughput screening of superior performing biomaterials. The proposed work will be accomplished through 1) utilizing computational fluid dynamics to design and simulate a variety of DEP microdevices as a means for separation of OHMs and, 2) construction and validation of the microdevice using two very different model microorganisms, Acidithiobacillus ferrooxidans and Cupriavidus necator. Development of a rapid screening tool for identification and separation of these high performing organisms would significantly enhance and accelerate screening and development of microorganisms used in biosorption of all types, not just specific to metals; providing a greener and potentially more cost effective technological solution to separation challenges.

1500815 Srivastava University of Shakespeare稀土元素（REE）被认为是关键材料，因为它们在各种商业部门中必不可少的用途以及它们的供应短缺和稀缺性。从废物流和报废产品中回收稀土元素是一项有吸引力的技术，但从这些材料中分离稀土元素可能成本高昂，并涉及有毒和危险的化学品。微生物有能力选择性地积累和压裂金属，包括稀土元素，但尚未部署作为一种分离技术的稀土元素。PI建议开发一种基于介电泳微流体分离（DEP）的高风险高回报平台技术。预计拟议的工作，通过提供一个高度适应性的手段，高通量筛选的上级性能的生物材料，改变筛选的生物材料。拟议的工作将通过1）利用计算流体动力学设计和模拟各种DEP微器件作为分离欧姆的手段，2）使用两种非常不同的模型微生物，嗜酸氧化亚铁硫杆菌和钩虫贪铜菌构建和验证微器件来完成。开发用于鉴定和分离这些高性能生物体的快速筛选工具将显著增强和加速用于所有类型生物吸附的微生物的筛选和开发，而不仅仅是特定于金属;为分离挑战提供更绿色和潜在更具有成本效益的技术解决方案。