Development of a rapid screening test for the detection of dihydroanatoxin-a

开发检测二氢虾毒素-a 的快速筛选试验

• 批准号: 10545266
• 负责人: LANCE P FORD
• 金额: $ 25.9万
• 依托单位: ATTOGENE
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-07-07 至 2025-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10545266
• 关键词: Address; Affect; Affinity; Anabaena; Anatoxins; Animals; Antibodies; Antigens; Aphanizomenon; Back; Binding; Biological Assay; Breathing; Buffers; Cardiovascular system; Cells; Cessation of life; Chronic; Complex; Consumption; Convulsions; Cyanobacterium; Cyanotoxin; Deposition; Detection; Development; Devices; Disease Outbreaks; Early Diagnosis; Eating; Ensure; Environment; Exposure to; Family; Feasibility Studies; Food; Goals; Goat; Gold; Gold Colloid; Health; Human; Human Resources; Immunoglobulin G; International; Laboratories; Liquid substance; Membrane; Methods; Monitor; Muscle; Muscle fasciculation; Nerve; Neurologic; Neurons; Neurotoxins; Nicotinic Receptors; Nutrient; Oryctolagus cuniculus; Phase; Poisoning; Preparation; Prevention; Procedures; Property; Protocols documentation; Public Health; Pyroxylin; Rapid screening; Reagent; Receptor Inhibition; Recreation; Respiratory Paralysis; Running; Sampling; Sensitivity and Specificity; Severities; Signal Transduction; Site; Sodium Chloride; Source; Swimming; Symptoms; Techniques; Technology; Temperature; Testing; Time; Toxic Environmental Substances; Toxic effect; Toxin; Training; Tumor Promotion; United States Department of Agriculture; Validation; Variant; Water; aerosolized; chemical synthesis; contaminated drinking water; cost; cost effective; detection assay; detection test; detector; field study; global environment; improved; innovation; instrumentation; lateral flow assay; manufacture; nanoparticle; performance tests; phase 2 study; prevent; programs; prototype; rapid detection; rapid test; receptor binding; screening; tool; water sampling

Project Summary Dihydroanatoxin (dhATX) derived from cyanobacteria is a rapidly emerging global problem that negatively impacts human and animal health. Poisoning induced by dhATX is characterized by a broad array of neurological and cardiovascular symptoms with extremes of severity which can lead to fatality. Therefore, efficient, and effective detection, prevention, control, and mitigation technologies are critically needed to prevent exposures. The only available technology to detect the presence of dhATX is expensive, time consuming, and labor intensive, allowing only sporadic testing to be performed. This lack of rapid, simple, inexpensive testing capability increases the chances of human and animal exposures and associated health problems. Based on our current progress in developing rapid assays for environmental toxins, we will develop a rapid, quantitative, and cost- effective receptor binding assay for detecting dhATX in the field setting. Our test platform will likely emerge as an integral component of monitoring programs domestically and internationally to improve dhATX detection, and will provide an effective tool for public health officials to act quickly to reduce dhATX exposures.

项目摘要 蓝藻来源的二氢Anatoxin(DhATX)是一个迅速出现的全球性问题，具有负面影响。 影响人类和动物的健康。由dhatx引起的中毒的特征是广泛的神经系统 以及严重到可导致死亡的心血管症状。因此，高效、 为了防止暴露，迫切需要有效的检测、预防、控制和缓解技术。 检测dhATX存在的唯一可用的技术是昂贵、耗时和费力的 密集的，只允许执行零星的测试。缺乏快速、简单、廉价的测试能力 增加人类和动物暴露及相关健康问题的机会。根据我们目前的情况 在开发环境毒素快速检测方法方面的进展，我们将开发一种快速、定量和成本- 现场检测dhATX的有效受体结合试验。我们的测试平台很可能会成为 作为国内和国际监测计划的组成部分，以改进dhATX检测；以及 将为公共卫生官员迅速采取行动减少dhATX暴露提供有效工具。