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Developing a Combined Vibrational Spectroscopic and Optical/Fluorescence Imaging Approach for Simultaneous Orientation and Activity Measurement of Immobilized Biomolecules

开发一种组合振动光谱和光学/荧光成像方法，用于固定生物分子的同步定向和活性测量

基本信息

批准号：
1505385
负责人：
Zhan Chen
金额：
$ 45万
依托单位：
Regents of the University of Michigan - Ann Arbor
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
2015
资助国家：
美国
起止时间：
2015-09-01 至 2019-08-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1505385&HistoricalAwards=false
关键词：
Developing Combined Vibrational Spectroscopic Optical

项目摘要

In this project funded by the Chemical Measurement and Imaging Program of the Chemistry Division, Professor Zhan Chen of The University of Michigan is constructing an analytical platform that combines several spectroscopic and imaging analytical techniques together for thepurpose of developing a general methodology to determine the structure of surface immobilized enzymes and peptides and measure their activities simultaneously. The understanding of structure-function relationships of surface immobilized biological molecules providesfundamental knowledge for the preparation of biocatalysts for biofuel production, biosensors, and biochips with improved performance. An outreach program developed by Professor Chen to teach surface chemistry to high school students promotes the students' interests in science. The broader impacts of this work include developing a widely applicable analytical platform, aiding in the development of better biocatalysts, biosensors, and biochips, and educating young researchers including femalestudents and postdocs.This project focuses on the development of a systematic approach by simultaneously applying linear/nonlinear vibrational spectroscopic and optical/fluorescence imaging techniques to elucidate the structure-function relationships of surface immobilized enzymes and peptides. An analytical platform which integrates sum frequency generation vibrational spectroscopy, attenuated total reflectance FTIR, optical microscopy, and fluorescence microscopy is constructed and allows for the simultaneous investigation of the structure and function of surface immobilized enzymes (e.g., beta-galactosidase and beta-glucosidase) and antimicrobial peptides(e.g., MSI-78).

在本项目中，美国密歇根大学的陈展教授正在构建一个将多种光谱和成像分析技术结合在一起的分析平台，目的是开发一种通用方法来确定表面固定化酶和肽的结构并同时测量其活性。了解表面固定化生物分子的结构-功能关系为制备生物催化剂、生物传感器和生物芯片提供了基础知识。陈教授为高中生开设的表面化学外展课程，促进了学生对科学的兴趣。这项工作的更广泛的影响包括开发一个广泛适用的分析平台，帮助开发更好的生物催化剂，生物传感器和生物芯片，该项目的重点是通过同时应用线性/非线性振动光谱和光学/荧光成像技术来阐明结构-表面固定化酶和肽的功能关系。构建了一种分析平台，其集成了和频产生振动光谱、衰减全反射FTIR、光学显微镜和荧光显微镜，并允许同时研究表面固定化酶（例如，β-半乳糖苷酶和β-葡糖苷酶）和抗微生物肽（例如，MSI-78）。