Developing a Combined Vibrational Spectroscopic and Optical/Fluorescence Imaging Approach for Simultaneous Orientation and Activity Measurement of Immobilized Biomolecules

在这个由化学系化学测量和成像计划资助的项目中，密歇根大学的詹陈教授正在构建一个将多种光谱和成像分析技术结合在一起的分析平台，目的是开发一种通用的方法学来确定表面固定化酶和多肽的结构并同时测量它们的活性。了解表面固定化生物分子的结构与功能关系，为制备用于生物燃料生产、生物传感器和性能改进的生物芯片的生物催化剂提供了基础知识。陈教授开发了一个外展项目，向高中生教授表面化学，以促进学生对科学的兴趣。这项工作的更广泛的影响包括开发一个广泛适用的分析平台，帮助开发更好的生物催化剂、生物传感器和生物芯片，以及教育年轻的研究人员，包括女学生和博士后。本项目专注于开发一种系统的方法，同时应用线性/非线性振动光谱和光学/荧光成像技术来阐明表面固定化酶和多肽的结构-功能关系。构建了一个集和频振动光谱、衰减全反射FTIR、光学显微镜和荧光显微镜于一体的分析平台，可以同时研究表面固定化酶(如β-半乳糖苷酶和β-葡萄糖苷酶)和抗菌肽(如MSI-78)的结构和功能。

In this project funded by the Chemical Measurement and Imaging Program of the Chemistry Division, Professor Zhan Chen of The University of Michigan is constructing an analytical platform that combines several spectroscopic and imaging analytical techniques together for thepurpose of developing a general methodology to determine the structure of surface immobilized enzymes and peptides and measure their activities simultaneously. The understanding of structure-function relationships of surface immobilized biological molecules providesfundamental knowledge for the preparation of biocatalysts for biofuel production, biosensors, and biochips with improved performance. An outreach program developed by Professor Chen to teach surface chemistry to high school students promotes the students' interests in science. The broader impacts of this work include developing a widely applicable analytical platform, aiding in the development of better biocatalysts, biosensors, and biochips, and educating young researchers including femalestudents and postdocs.This project focuses on the development of a systematic approach by simultaneously applying linear/nonlinear vibrational spectroscopic and optical/fluorescence imaging techniques to elucidate the structure-function relationships of surface immobilized enzymes and peptides. An analytical platform which integrates sum frequency generation vibrational spectroscopy, attenuated total reflectance FTIR, optical microscopy, and fluorescence microscopy is constructed and allows for the simultaneous investigation of the structure and function of surface immobilized enzymes (e.g., beta-galactosidase and beta-glucosidase) and antimicrobial peptides(e.g., MSI-78).