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Ion Channel Probes for Scanning Ion Conductance Microscopy

用于扫描离子电导显微镜的离子通道探针

基本信息

批准号：
1507341
负责人：
Lane Baker
金额：
$ 44万
依托单位：
Indiana University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2015
资助国家：
美国
起止时间：
2015-07-15 至 2018-06-30
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1507341&HistoricalAwards=false
关键词：
Ion Channel Probes Scanning Conductance

项目摘要

With this award, the Chemical Measurement and Imaging program of the Chemistry Division is funding Professor Lane Baker at Indiana University to develop chemical/biochemical sensors which consist of lipid bilayers and ion channels suspended at the tip of a glass capillary, called ion channel probes (or ICPs). The sensor will be combined with scanning ion conductance microscopy (SICM), a probe imaging platform, to enable spatial location of the sensor on the order of 50-100 nm. By incorporating ion channels with selective responses, sensors that respond to unique analytes will be developed. The positionable ICP will be tested on porous membranes and will enable measurement of small molecules and ions at subcellular scales and will enable new studies of cellular communication. As such, it is expected that the tools developed in this work that will have broad application in chemical biology. Students trained in this project will learn state-of-the-art fabrication, measurement and characterization protocols, providing them with high level technical skills for the current workforce upon their graduation. In addition, efforts will be made to improve chemistry faculty engagement in evidence-based teaching methods. The proposed research will provide new routes to chemically selective imaging methods, even at single molecule levels, with scanning ion conductance microscopy. This overcomes a significant drawback of scanning ion conductance microscopy; namely, the lack of chemical information collected. The use of inherently selective and sensitive ion channels will impart chemical sensing abilities to scanning ion conductance microscopy. As described, ICP-SICM promises to increase the reliable operation, signal and time response of sniffer patches, which will, in turn, inform the biochemical process they are commonly used to study.

有了这个奖项，化学部的化学测量和成像计划正在资助印第安纳州大学的Lane Baker教授开发化学/生物化学传感器，该传感器由脂质双层和悬浮在玻璃毛细管尖端的离子通道组成，称为离子通道探针（或ICP）。该传感器将与扫描离子电导显微镜（SICM），探针成像平台相结合，使传感器的空间位置在50-100 nm的顺序。通过将离子通道与选择性反应，传感器，响应独特的分析物将被开发。可定位的ICP将在多孔膜上进行测试，并将能够在亚细胞尺度上测量小分子和离子，并将能够对细胞通讯进行新的研究。因此，预计这项工作中开发的工具将在化学生物学中得到广泛应用。在该项目中接受培训的学生将学习最先进的制造，测量和表征协议，为他们毕业后的现有劳动力提供高水平的技术技能。此外，将努力提高化学教师参与循证教学方法。这项研究将为化学选择性成像方法提供新的途径，即使是在单分子水平上，扫描离子电导显微镜。这克服了扫描离子电导显微镜的一个显著缺点，即缺乏收集的化学信息。固有的选择性和敏感的离子通道的使用将赋予扫描离子电导显微镜的化学传感能力。如前所述，ICP-SICM有望提高嗅探器贴片的可靠操作、信号和时间响应，这反过来又会告知它们通常用于研究的生化过程。