Increasing Amino Acid Diversity in In Vitro Translation with Expanded Genetic Alphabets

通过扩展遗传字母表增加体外翻译中的氨基酸多样性

基本信息

项目摘要

This project is jointly funded by the Chemistry of Life Processes Program in the Division of Chemistry in the Directorate of Mathematical and Physical Sciences and the Molecular Genetic Mechanisms Cluster in the Division of Molecular and Cellular Biosciences in the Directorate for Biological Sciences.With this award, the Chemistry of Life Processes Program in the Chemistry Division is funding Dr. Steven Benner of The Westheimer Institute for Science and Technology (TWIST) within the Foundation for Applied Molecular Evolution to expand the power of biologically created proteins. The next generation of biotechnology will use components of living systems to create unnatural biopolymers, including proteins that contain unnatural amino acids. This award will develop this biotechnology, at the same time as deepening our understanding of how proteins are made in living cells. Proteins produced by biotechnology find use in products as diverse as pharmaceuticals, cosmetics, industrial manufacture, and laundry detergents. Adding unnatural amino acids to these may open up for exploration a horizon of proteins having properties that natural proteins cannot reach. The project trains undergraduate students in methods of cutting-edge technology development.The experiments designed for this project combine chemical synthesis with molecular biology, laboratory evolution, and bioinformatics to gain control over each step in the in vitro synthesis of proteins. This includes developing new ways to put unnatural amino acids onto unnatural transfer RNA molecules by directed evolution of charging catalysts, chemically synthesizing new nucleic acid analogs to manage infidelity in RNA biosynthesis and translation, and understanding of how translation systems exploit auxiliary factors to determine the starting point and ending point of messenger RNA translation. These are coupled with models for the ribosome, recently supported by X-ray crystallography, to build a complex picture of synthetic biology in this space.
该项目由数学和物理科学局化学司生命过程化学项目和生物科学局分子和细胞生物科学司分子遗传机制集群联合资助。化学部生命过程化学项目将资助韦斯特海默科学技术研究所 (TWIST) 的 Steven Benner 博士 应用分子进化来扩展生物产生的蛋白质的力量。下一代生物技术将利用生命系统的组成部分来制造非天然生物聚合物,包括含有非天然氨基酸的蛋白质。该奖项将开发这种生物技术,同时加深我们对活细胞中蛋白质如何制造的理解。通过生物技术生产的蛋白质可用于药品、化妆品、工业制造和洗衣粉等多种产品。在其中添加非天然氨基酸可能会为探索具有天然蛋白质无法达到的特性的蛋白质开辟新的视野。该项目对本科生进行前沿技术开发方法的培训。该项目设计的实验将化学合成与分子生物学、实验室进化和生物信息学相结合,以控制蛋白质体外合成的每个步骤。这包括开发新方法,通过电荷催化剂的定向进化将非天然氨基酸置于非天然转移RNA分子上,化学合成新的核酸类似物来管理RNA生物合成和翻译中的不忠实性,以及了解翻译系统如何利用辅助因素来确定信使RNA翻译的起点和终点。 这些与最近得到 X 射线晶体学支持的核糖体模型相结合,构建了该领域合成生物学的复杂图景。

项目成果

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Steven Benner其他文献

Steven Benner的其他文献

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{{ truncateString('Steven Benner', 18)}}的其他基金

Sedimentary Minerals, Organic Chemical Transformations, and the Origin of Life
沉积矿物、有机化学转化和生命起源
  • 批准号:
    2213438
  • 财政年份:
    2022
  • 资助金额:
    $ 48万
  • 项目类别:
    Standard Grant
Collaborative Research: Synthetic and Systems Biology Approaches to Semi-synthetic Cells with Expanded DNA Alphabets
合作研究:具有扩展 DNA 字母表的半合成细胞的合成和系统生物学方法
  • 批准号:
    2123995
  • 财政年份:
    2021
  • 资助金额:
    $ 48万
  • 项目类别:
    Standard Grant
Darwinism from Artificial Genomes
来自人工基因组的达尔文主义
  • 批准号:
    1939086
  • 财政年份:
    2019
  • 资助金额:
    $ 48万
  • 项目类别:
    Standard Grant
RoL:EAGER: DESYN-C3. Bottom-up Synthetic Cells Capable of Darwinism, the Archetypal Trait of Life
ROL:渴望:DESYN-C3。
  • 批准号:
    1844290
  • 财政年份:
    2018
  • 资助金额:
    $ 48万
  • 项目类别:
    Standard Grant
Firebug: A Living Cell that Propagates Plasmids Built from an Artificial Genetic System
Firebug:一种由人工遗传系统构建的可传播质粒的活细胞
  • 批准号:
    1412869
  • 财政年份:
    2014
  • 资助金额:
    $ 48万
  • 项目类别:
    Standard Grant
Conference: 2014 Origins of Life, January 12-17, 2014 in Galveston, TX
会议:2014 年生命起源,2014 年 1 月 12 日至 17 日,德克萨斯州加尔维斯顿
  • 批准号:
    1338585
  • 财政年份:
    2013
  • 资助金额:
    $ 48万
  • 项目类别:
    Standard Grant
OPUS: Geological, Environmental, and Chemical Biology
作品:地质、环境和化学生物学
  • 批准号:
    0717335
  • 财政年份:
    2007
  • 资助金额:
    $ 48万
  • 项目类别:
    Continuing Grant
Quantitating In Vitro Selection
定量体外选择
  • 批准号:
    0403139
  • 财政年份:
    2003
  • 资助金额:
    $ 48万
  • 项目类别:
    Standard Grant
Quantitating In Vitro Selection
定量体外选择
  • 批准号:
    0213575
  • 财政年份:
    2002
  • 资助金额:
    $ 48万
  • 项目类别:
    Standard Grant
Self-assembling Nanostructures from An Expanded Genetic Information System (AEGIS)
来自扩展遗传信息系统(AEGIS)的自组装纳米结构
  • 批准号:
    9871880
  • 财政年份:
    1998
  • 资助金额:
    $ 48万
  • 项目类别:
    Standard Grant

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Amplification of chiral recognition and discrimination among amino-acid-based nanoscale ions during assembly induced by electrostatic interaction
静电相互作用诱导组装过程中氨基酸纳米级离子之间手性识别和辨别的放大
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  • 批准号:
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    2024
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Collaborative Research: Phylogenetic and Physiological Characterization of Amino Acid Nitrogen Isotopes in Phytoplankton
合作研究:浮游植物氨基酸氮同位素的系统发育和生理学特征
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