RAPID: Collaborative Research: Survival of Ebolavirus in the Water Environment: Surrogate Development and Disinfection Effectiveness

快速：合作研究：埃博拉病毒在水环境中的生存：替代物的开发和消毒效果对埃博拉病毒的命运及其在环境中的生存知之甚少。已观察到灵长类动物通过非直接接触感染，进一步提高了环境感染途径的可能性。作为该RAPID的初始阶段，将根据已公布的埃博拉病毒在液体培养基中的存活数据开发埃博拉病毒替代品。其次，将在环境水域（包括水和废水）以及存在水和废水消毒剂的情况下评估这些替代品的存活率。这项工作将对更广泛的环境、公共卫生和风险评估界有价值，因为它提供了可以进行研究（例如气溶胶和污染物生存研究）的现实替代品。建议的废物快速处理机制，可适时为废物处理提供资讯。这项工作还将有助于动员和通报对适当废物处理的反应。鉴于获得BSL4的机会有限，这一方法是近期内最合理的应对措施，可为埃博拉病毒在水环境中的生存情况以及随后的风险评估提供信息。该项目的实验工作将在匹兹堡大学进行。数据和统计分析将在德雷塞尔大学进行。拟议的研究将通过告知如何正确处理受埃博拉病毒污染的废物而造福社会。拟议的工作将通过期刊出版物和会议记录广泛传播。拟议的研究将填补关于埃博拉病毒污染废物的生存和适当处理的现有知识空白。这项研究还将开发和验证可被更广泛的环境工程和公共卫生研究界用于埃博拉病毒生存研究的替代品。

CBET 1508415BibbyCBET 1507285HaasRAPID: Collaborative Research: Survival of Ebola virus in the Water Environment: Surrogate Development and Disinfection EffectivenessLittle is known about the fate of Ebola virus and its survival in the environment. Infection in primates has been observed from non-direct contact, raising further the possibility of an environmental infection route. As an initial phase of this RAPID, Ebola virus surrogates, based upon the published data of Ebola virus survival in liquid media, will be developed. Second, the survival of these surrogates will be evaluated in environmental waters (including water and wastewater) and in the presence of water and wastewater disinfectants. This work will be valuable to the broader environmental, public health, and risk assessment communities by providing access to realistic surrogates with which studies (e.g. aerosol and fomite survival studies) may be conducted. The proposed RAPID mechanism is appropriate to inform proper waste handling in a timely manner. This work will also serve to mobilize and inform a response to proper waste handling. Given limited BSL4 access, this approach is the most reasonable response in the near term to inform Ebola virus survival in the water environment, and subsequent risk assessment. Experimental work on this project will be conducted at the University of Pittsburgh. Data and statistical analysis will be conducted at Drexel University. The proposed research will benefit society through informing proper handling of Ebola virus contaminated waste. The proposed work will be disseminated broadly through journal publications and conference proceedings. The proposed research will fill in an existing knowledge gap in the survival and proper handling of Ebola virus contaminated waste. This research will also develop and verify surrogates that may be used by the broader environmental engineering and public health research communities for Ebola virus survival studies.