Hybrid Nanoprobes Of Peptide-Functionalized Quantum Dots And Magnetic Nanocrystals, Interfaced Via Amphiphilic Multifunctional Polymer Ligands

通过两亲性多功能聚合物配体连接的肽功能化量子点和磁性纳米晶体的混合纳米探针

• 批准号: 1508501
• 负责人: Hedi Mattoussi
• 金额: $ 48万
• 依托单位: Florida State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-07-15 至 2019-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1508501&HistoricalAwards=false
• 关键词: Hybrid; Nanoprobes; Peptide; Functionalized; Quantum

With this award, the Macromolecular, Supramolecular and Nanochemistry Program of the Chemistry Division is funding Drs. Hedi Mattoussi and Debra Fadool at the Florida State University to develop strategies to modify the surfaces of inorganic nanomaterials so that they can bind with biological systems. This will yield systems that can be used to probe specific biological species and monitor targeted biological processes without affecting the integrity of the host system. To meet this goal, new multifunctional polymers that strongly coordinate onto various nanomaterials will be designed, providing water-compatible nanoprobes that are easy to introduce into biological media/systems. The work at Florida State University provides a good example where nanotechnology is used to address important problems in biology. The present project is interdisciplinary in nature and it provides opportunities for training graduate and undergraduate students in areas such as growth and functionalization of nanocrystals, peptide design and synthesis, protein expression, and several other analytical techniques. Prof. Mattoussi and co-workers will also carry out outreach activities to undergraduate students, including students from underrepresented groups.The project focuses on developing amphiphilic polymers as surface ligands that can tightly coordinate onto the surfaces of inorganic nanomaterials, including magnetic nanoparticles, luminescent quantum dots and gold nanoparticles. The chemical design exploits the effectiveness of the nucleophilic addition reaction to allow the simultaneous insertion of hydrophilic moieties, metal-coordinating groups and reactive functions on the same polymer platform. By combining multi-anchoring groups with hydrophilic and biologically-reactive functionalities in the same amphiphilic polymer, our design will enhance the ligand-to-nanoparticle binding, while facilitating their coupling to various target molecules. This approach will allow conjugation of QDs and magnetic nanocrystals to several target molecules, including dopamine and the margatoxin (MgTx) peptide. These resulting conjugates will be used to develop specific biosensors. In particular QD-dopamine conjugates will provide platforms that can target iron ions and cysteine-rich proteins in vitro and in cell cultures. Similarly, nanoparticle-MgTx conjugates will be used to control the current flow through the Kv1.3 potassium channels of mitral cells.

有了这个奖项，化学部的大分子，超分子和纳米化学计划正在资助佛罗里达州立大学的Hedi Mattoussi和Debra Fadool博士开发修改无机纳米材料表面的策略，使它们能够与生物系统结合。这将产生可用于探测特定生物物种和监测目标生物过程而不影响宿主系统完整性的系统。为了实现这一目标，将设计与各种纳米材料牢固配合的新型多功能聚合物，提供易于引入生物介质/系统的水相容性纳米探针。 佛罗里达州立大学的工作提供了一个很好的例子，纳米技术被用来解决生物学中的重要问题。 目前的项目是跨学科的性质，它提供了培训研究生和本科生的机会，如纳米晶体的生长和功能化，肽的设计和合成，蛋白质的表达，以及其他几种分析技术。Mattoussi教授和同事们还将开展针对本科生的外展活动，包括来自代表性不足群体的学生。该项目的重点是开发两亲聚合物作为表面配体，可以紧密配位到无机纳米材料的表面，包括磁性纳米颗粒，发光量子点和金纳米颗粒。化学设计利用亲核加成反应的有效性，允许在同一聚合物平台上同时插入亲水部分、金属配位基团和反应性官能团。通过将多锚定基团与亲水性和生物反应性官能团结合在同一两亲性聚合物中，我们的设计将增强配体与纳米颗粒的结合，同时促进它们与各种靶分子的偶联。这种方法将允许量子点和磁性纳米晶体与几种靶分子结合，包括多巴胺和玛格毒素（MgTx）肽。这些得到的缀合物将用于开发特定的生物传感器。特别地，QD-多巴胺缀合物将提供可以在体外和细胞培养物中靶向铁离子和富含半胱氨酸的蛋白质的平台。类似地，纳米颗粒-MgTx缀合物将用于控制通过二尖瓣细胞的Kv1.3钾通道的电流。

项目成果

N-Heterocyclic Carbene-Stabilized Gold Nanoparticles: Mono- Versus Multidentate Ligands

• DOI: 10.1021/acs.chemmater.0c03918
• 发表时间: 2021-01
• 期刊: Chemistry of Materials
• 影响因子: 8.6
• 作者: N. A. Nosratabad;Zhicheng Jin;Liang Du;Mannat Thakur;H. Mattoussi

Luminescent Quantum Dots Stabilized by N-Heterocyclic Carbene Polymer Ligands

• DOI: 10.1021/jacs.0c10592
• 发表时间: 2021-01-15
• 期刊: JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
• 影响因子: 15
• 作者: Du, Liang;Nosratabad, Neda Arabzadeh;Mattoussi, Hedi
• 通讯作者: Mattoussi, Hedi