RUI: Spatially and Compositionally Gradient Scaffolds for Anterior Cruciate Ligament

RUI：前十字韧带的空间和成分梯度支架

• 批准号: 1510479
• 负责人: Derrick Dean
• 金额: $ 45万
• 依托单位: Alabama State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-09-15 至 2020-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1510479&HistoricalAwards=false
• 关键词: RUI; Spatially; Compositionally; Gradient; Scaffolds

PI: Dean, Derrick R.Proposal Number: 1510479The human anterior cruciate ligament (ACL) is ruptured over 200,000 times per year (or an incidence of 1 in 3000) in the United States, resulting in over $1 billion of medical expenses. The gold standard for surgical repair is the patellar tendon autograft, but this treatment is far from optimal due to lengthy recovery time, potential for developing arthritis, associated donor site morbidity, and degenerative joint disease. These limitations have prompted the need for a tissue engineered solution. This study proposes to use a multidisciplinary approach to provide a fundamental understanding of the design and fabrication of a scaffold (a temporary structure made of a biodegradable polymer that facilitates the growth of cells and tissue) that mimics and facilitates the development of the four tissue types found in the ACL structure. Students will be involved in the research, and several courses will benefit from the knowledge generated by this project. The human anterior cruciate ligament (ACL) is ruptured over 200,000 times per year (or an incidence of 1 in 3000) in the United States, resulting in over $1 billion of medical expenses. The conventional surgical repair involves autografting the patellar tendon autograft, however shortcomings of this approach include long recovery time, potential for developing arthritis, associated donor site morbidity, and degenerative joint disease. These limitations underscore the need for a tissue engineered solution. This study proposes to use a multidisciplinary approach which provides a fundamental understanding of the evolution of a hierarchical, spatially organized 2-dimensional biomimetic scaffold designed to facilitate the development of the four tissue types found in a ligament-to-bone interface. The objectives of this study are: To utilize inkjet printing to prepare hierarchical, spatially organized structures that can be used for bone-ligament interfaces; to characterize the morphology, composition, mechanical behavior and immunochemistry across the bone-to-ligament interface; and to understand the material-cell interactions across the gradient structure. The fundamental knowledge gained from this study will significantly impact the engineering of bone-ligament interfaces and other applications where gradient structures are present. Program resources will be leveraged with the existing REU program at Alabama State University and the NSF/Louis Stokes Alliance for Minority Participation program (LSAMP), to maximize the involvement of undergraduate students from underrepresented groups. Several courses will benefit from knowledge generated from the research, and students involved will have opportunities to present their research at national and regional conferences.

主要研究者：Dean，Derrick R.提案编号：1510479在美国，人类前交叉韧带（ACL）每年断裂超过20万次（或发生率为1/3000），导致超过10亿美元的医疗费用。手术修复的金标准是髌腱自体移植，但由于恢复时间长，可能发生关节炎，相关供体部位发病率和退行性关节疾病，这种治疗远非最佳。这些限制促使了对组织工程解决方案的需求。 本研究建议使用多学科方法来提供对支架（由可生物降解聚合物制成的临时结构，可促进细胞和组织的生长）的设计和制造的基本理解，该支架模拟并促进ACL结构中发现的四种组织类型的发育。 学生将参与研究，几门课程将受益于该项目产生的知识。 在美国，人类前交叉韧带（ACL）每年断裂超过200，000次（或发生率为1/3000），导致超过10亿美元的医疗费用。传统的手术修复涉及自体髌腱移植物的自体移植，然而这种方法的缺点包括恢复时间长、发展关节炎的可能性、相关的供体部位发病率和退行性关节疾病。这些限制强调了对组织工程解决方案的需要。 本研究建议使用多学科的方法，提供了一个层次的，空间上有组织的2维仿生支架，旨在促进韧带-骨界面中发现的四种组织类型的发展的演变的基本理解。 本研究的目的是：利用喷墨打印制备可用于骨-韧带界面的分层、空间组织结构;表征骨-韧带界面的形态、组成、机械行为和免疫化学;并了解梯度结构中的材料-细胞相互作用。 从这项研究中获得的基础知识将显着影响骨韧带界面的工程和梯度结构存在的其他应用。计划资源将利用现有的REU计划在亚拉巴马州立大学和NSF/路易斯斯托克斯联盟少数民族参与计划（LSAMP），以最大限度地提高来自代表性不足的群体的本科生的参与。 几门课程将受益于研究产生的知识，参与的学生将有机会在国家和区域会议上介绍他们的研究。