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The "SEISMIC" facility for Spatially rEsolved sIngle and Sub-cellular oMICs

用于空间解析单细胞和亚细胞组学的“SEISMIC”设施

基本信息

批准号：
BB/W019116/1
负责人：
Melanie Bailey
金额：
$ 195.83万
依托单位：
University of Surrey
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2023
资助国家：
英国
起止时间：
2023 至无数据
项目状态：
未结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FW019116%2F1
关键词：
SEISMIC facility Spatially rEsolved sIngle

项目摘要

An exciting innovation in the analysis of a biological system is sampling on a very small nanoscale using a specialised needle (nano capillary sampling). Cells are located under a specialised (confocal) microscope and cells or even small parts of cells can be sampled. This uniquely provides spatial information and can be performed on living cells which will allow scientists to understand important biological phenomenon such as how cells communicate with each other and how cells become cancerous. Our resource which we have called SEISMIC will provide an automated platform based on nano capillary sampling. We will work with users within the UK research community to extract single cells and their sub- cellular compartments under a microscope. The extracted cellular materials can be analysed using a variety of approaches such as mass spectrometry which separates molecules by mass and charge to profile for example drugs, metabolites and lipids or apply other techniques to profile nucleic acids. This will allow an unprecedented view of cells and can be applied to a plethora of biological questions which inform us on for example the rules of life or how pathogens cause disease or how cells age, information which can be harnessed to develop new therapeutic interventions which will ultimately benefit society.The SEISMIC resource will be available free of charge to BBSRC users for the first 36 months, either with or without downstream mass spectrometry analysis. Users will be able to perform their experiments using the facilities at Surrey, and hands on access to SEISMIC will be supported through travel grants. This facility will be the first of its kind in the UK and therefore will play a role in maintaining world leading science in the UK.

生物系统分析中的一项令人兴奋的创新是使用专用针在非常小的纳米尺度上进行采样（纳米毛细管采样）。细胞位于专门的（共聚焦）显微镜下，细胞或甚至细胞的一小部分可以取样。这独特地提供了空间信息，并且可以在活细胞上进行，这将使科学家能够了解重要的生物现象，例如细胞如何相互交流以及细胞如何癌变。我们称之为SEISMIC的资源将提供基于纳米毛细管采样的自动化平台。我们将与英国研究界的用户合作，在显微镜下提取单细胞及其亚细胞区室。可以使用多种方法分析提取的细胞材料，例如质谱法，其通过质量和电荷分离分子以分析例如药物、代谢物和脂质，或应用其他技术来分析核酸。这将为我们提供一个前所未有的细胞视角，并可应用于大量的生物学问题，例如生命规则、病原体如何导致疾病或细胞如何衰老，这些信息可用于开发新的治疗干预措施，最终造福社会。SEISMIC资源将在前36个月免费提供给BBSRC用户，可以进行或不进行下游质谱分析。用户将能够使用萨里的设施进行实验，并通过旅行赠款支持对SEISMIC的实际访问。该设施将是英国第一个此类设施，因此将在英国保持世界领先的科学方面发挥作用。

项目成果

期刊论文数量（4）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Single-Cell Lipidomics Using Analytical Flow LC-MS Characterizes the Response to Chemotherapy in Cultured Pancreatic Cancer Cells.

DOI：
10.1021/acs.analchem.3c02854
发表时间：
2023-10-03
期刊：
ANALYTICAL CHEMISTRY
影响因子：
7.4
作者：
Saunders, Kyle D. G.;von Gerichten, Johanna;Lewis, Holly-May;Gupta, Priyanka;Spick, Matt;Costa, Catia;Velliou, Eirini;Bailey, Melanie J.
通讯作者：
Bailey, Melanie J.

Expanding the boundaries of atomic spectroscopy at the single-cell level: critical review of SP-ICP-MS, LIBS and LA-ICP-MS advances for the elemental analysis of tissues and single cells.

DOI：
10.1007/s00216-023-04721-8
发表时间：
2023-11
期刊：
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
影响因子：
4.3
作者：
Davison, Claire;Beste, Dany;Bailey, Melanie;Felipe-Sotelo, Monica
通讯作者：
Felipe-Sotelo, Monica

Nanocapillary sampling coupled to liquid chromatography mass spectrometry delivers single cell drug measurement and lipid fingerprints.

DOI：
10.1039/d2an01732f
发表时间：
2023-02-27
期刊：
ANALYST
影响因子：
4.2
作者：
Lewis, Holly-May;Gupta, Priyanka;Saunders, Kyle D. G.;Briones, Shazneil;von Gerichten, Johanna;Townsend, Paul A.;Velliou, Eirini;Beste, Dany J. V.;Cexus, Olivier;Webb, Roger;Bailey, Melanie J.
通讯作者：
Bailey, Melanie J.

Spatial single cell metabolomics: Current challenges and future developments.