The "SEISMIC" facility for Spatially rEsolved sIngle and Sub-cellular oMICs

生物系统分析中的一项令人兴奋的创新是使用专门的针(纳米毛细管采样)在非常小的纳米尺度上进行采样。细胞被放置在特殊的(共焦)显微镜下，可以对细胞甚至细胞的一小部分进行采样。这一技术提供了独特的空间信息，可以在活细胞上进行，这将使科学家能够了解重要的生物现象，如细胞如何相互通信以及细胞如何变得癌症。我们的资源，我们称之为地震，将提供一个基于纳米毛细管采样的自动化平台。我们将与英国研究界的用户合作，在显微镜下提取单个细胞及其亚细胞室。提取的细胞材料可以使用各种方法进行分析，例如按质量和电荷分离分子的质谱仪，以分析药物、代谢物和脂类，或应用其他技术来分析核酸。这将允许对细胞的前所未有的看法，并可以应用于过多的生物学问题，这些问题告诉我们例如生命规则或病原体如何导致疾病或细胞如何老化，这些信息可以被利用来开发最终将造福社会的新的治疗干预措施。地震资源将在前36个月免费提供给BBSRC用户，无论是否有下游的质谱分析。用户将能够使用萨里的设施进行他们的实验，并将通过旅行补助金支持亲身接触地震。该设施将是英国第一个此类设施，因此将在保持英国世界领先的科学方面发挥作用。

An exciting innovation in the analysis of a biological system is sampling on a very small nanoscale using a specialised needle (nano capillary sampling). Cells are located under a specialised (confocal) microscope and cells or even small parts of cells can be sampled. This uniquely provides spatial information and can be performed on living cells which will allow scientists to understand important biological phenomenon such as how cells communicate with each other and how cells become cancerous. Our resource which we have called SEISMIC will provide an automated platform based on nano capillary sampling. We will work with users within the UK research community to extract single cells and their sub- cellular compartments under a microscope. The extracted cellular materials can be analysed using a variety of approaches such as mass spectrometry which separates molecules by mass and charge to profile for example drugs, metabolites and lipids or apply other techniques to profile nucleic acids. This will allow an unprecedented view of cells and can be applied to a plethora of biological questions which inform us on for example the rules of life or how pathogens cause disease or how cells age, information which can be harnessed to develop new therapeutic interventions which will ultimately benefit society.The SEISMIC resource will be available free of charge to BBSRC users for the first 36 months, either with or without downstream mass spectrometry analysis. Users will be able to perform their experiments using the facilities at Surrey, and hands on access to SEISMIC will be supported through travel grants. This facility will be the first of its kind in the UK and therefore will play a role in maintaining world leading science in the UK.