UNS: Fractionation, purification, and analysis of gases in microbubbles

1512544巴苏韦恩州立大学在生命科学研究中，化学分离、分馏和分析构成了一个完整的分析工作流程。当对液体样品执行这样的工作流程时，可以使用传统的馏分收集工具，例如吸管、试管和托盘。然而，对于气体，这些基本操作更具挑战性，因为气体不容易控制。这项提议的目标是创造一种平台技术，可以使用气相色谱(GC)分离气体，然后将它们封装成气泡，以便可以离线分析或操纵。如果成功，拟议的多相系统将实现类似于液体系统的气体净化和分析的新工作流程。从分离科学的角度来看，这种封装技术可以在10ms的时间分辨率和1NL的馏分体积下封装数千个气体馏分，大约是依赖于低温圈闭的商业预备GC系统的1000倍。这可能会对PREP-GC和多维GC产生变革性的影响，在这些领域，组分收集的分辨率和可扩展性至关重要。该平台还将提供一种方法，通过使用对气泡形式的样品进行操作的化学试剂分析来筛选气体馏分的反应性。这项拟议的工作将探索基本问题，包括载液中气体的遏制，以及分析封装气体的方法。从更广泛的角度来看，这个拟议的系统可能会对多维气体分析产生重大影响，多维气体分析是环境监测、国土安全和食品分析中的常见工作流程。该推广计划将在密歇根科学中心(MSC)部署低成本的“果冻”微流控试剂盒，目标是增加底特律地区对STEM相关学科的兴趣。来自拟议研究的科学知识将整合到PI的研究生级别的生物微机械和微流变学课程中。

1512544BasuWayne State UniversityChemical separation, fractionation, and assay comprise an integral analytical workflow in life sciences research. When executing such workflows with liquid samples, conventional fraction collection tools such as pipettors, test tubes, and trays can be used. With gases, however, these basic operations are more challenging since gases are not easily contained. The objective of this proposal is to create a platform technology which can separate gases using gas chromatography (GC), and then and encapsulate them into bubbles so that they can be analyzed or manipulated offline. If successful, the proposed multiphase system would enable new workflows for gas purification and analysis analogous to their liquid counterparts. From the perspective of separation science, the encapsulation technique can encapsulate thousands of gas fractions at time resolution of 10ms and fraction volumes 1nL, roughly 1000X better than commercial preparatory-GC systems, which rely on cryogenic traps. This can can have transformative impact on prep-GC and multidimensional GC, where the resolution and scalability of fraction collection is critical. This platform would also provide a way to screen the reactivity of gas fractions by using chemical reagent assays which operate on samples in the bubble format. The proposed work will explore fundamental issues, including the containment of gases in a carrier fluid, and methods to analyze encapsulated gasses. From a broader perspective, this proposed system may substantially impact multidimensional gas analysis, a common workflow in environmental monitoring, homeland security, and food analysis. The outreach plan will deploy low-cost "jello" microfluidic kits at the Michigan Science center (MSC), with the goal of increasing interest in STEM-related disciplines in the Detroit area. Scientific knowledge from the proposed research will be integrated into the PI's graduate level BioMEMS and microfluidics course.