EAPSI: Using Ancient DNA to Determine Biological Kinship in a Bronze Age Cemetery, China

EAPSI：利用古代 DNA 确定中国青铜时代墓地的生物亲缘关系

• 批准号: 1515533
• 负责人: Alyssa Bader
• 金额: $ 0.51万
• 依托单位: Bader Alyssa
• 依托单位国家: 美国
• 项目类别: Fellowship Award
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-06-01 至 2016-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1515533&HistoricalAwards=false
• 关键词: EAPSI; Using; Ancient; DNA; Determine

The analysis of ancient DNA (aDNA) recovered from individuals excavated from archaeological contexts has become a valuable tool for improving understanding of past populations and their cultures. This research project will use aDNA as a tool to delineate the biological kinship relationships present, if any, amongst individuals excavated from the Hanshu II Burial Group 1 of the Houtaomuga cemetery in Da?an, Jilin Province, China. Analysis of genetic relatedness between the sampled individuals will determine if individuals buried at Houtaomuga are biologically related and, if so, whether kinship is socially stratified within the cemetery. As this culture marks a period of social transition from a pastoral economy to mixed agriculture subsistence, this is a particularly important research site for understanding social organization. The research will be conducted under the advisement of Dr. Yinqui Cui at the Jilin University Ancient DNA Laboratory, in collaboration with the Chinese Center for Frontier Archaeology at Jilin University. During this period, individuals were buried in either joint burial catacomb tombs or single burial earthen graves, indicating social stratification between individuals. The proposed project will analyze aDNA extracted from individuals excavated from both tomb types to reveal the probability of biological kinship within burials, as well as the biological relationship, if any, between individuals interred in high-status and lower-status burials. The hypervariable region (HVRI) of the mitochondrial DNA (mtDNA) will be sequenced for each individual, as well as the Y-chromosome of male individuals. If the rate of aDNA recovery is sufficient, analysis of biparental autosomal genetic markers will also be conducted. No genetic analysis of intracemetery kinship has thus far been performed on remains from the Hanshu II cultural period of Bronze Age China. Thus, this project will contribute to a more nuanced understanding of Hanshu II culture at a time of economically significant social transition. This NSF EAPSI award supports the research of a U.S. graduate student and is funded in collaboration with the Chinese Ministry of Science and Technology.

对从考古环境中挖掘出的个体中回收的古代 DNA (aDNA) 进行分析已成为增进对过去人群及其文化的了解的宝贵工具。该研究项目将使用aDNA作为工具来描述中国吉林省大安市后陶木嘎墓地汉书二号墓葬群1出土个体之间存在的生物亲缘关系（如果有的话）。对抽样个体之间的遗传相关性进行分析将确定埋葬在后陶木嘎的个体是否具有生物学相关性，如果是，则确定墓地内的亲属关系是否存在社会分层。由于这种文化标志着从游牧经济到混合农业生存的社会转型时期，因此这是理解社会组织的一个特别重要的研究场所。该研究将在吉林大学古代DNA实验室崔银奎博士的指导下，与吉林大学中国前沿考古中心合作进行。这一时期的个人埋葬方式要么是合葬地下墓穴，要么是单葬土坟，显示了个人之间的社会分层。拟议的项目将分析从两种墓葬类型中出土的个体中提取的DNA，以揭示墓葬中生物亲属关系的可能性，以及高地位和低地位墓葬中埋葬的个体之间的生物关系（如果有的话）。 将对每个个体的线粒体 DNA (mtDNA) 的高变区 (HVRI) 以及男性个体的 Y 染色体进行测序。如果aDNA回收率足够，还将进行双亲常染色体遗传标记分析。迄今为止，尚未对中国青铜时代汉书二文化时期的遗骸进行墓地内亲属关系的遗传分析。因此，该项目将有助于在经济重大的社会转型时期对《汉书 II》文化有更细致的了解。该 NSF EAPSI 奖项支持美国研究生的研究，并由与中国科学技术部合作资助。