EAPSI:Electron transfer characteristics of thermophilic electrode-respiring bacteria

EAPSI：嗜热电极呼吸细菌的电子传递特性

• 批准号: 1515597
• 负责人: Adan Medina
• 金额: $ 0.01万
• 依托单位: Medina Adan
• 依托单位国家: 美国
• 项目类别: Fellowship Award
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-06-01 至 2016-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1515597&HistoricalAwards=false
• 关键词: EAPSI; Electron; transfer; characteristics; thermophilic

Biofilm-forming electrogenic bacteria are so called due to their ability to interact with solid electrodes. These electrochemically active biofilms (EABs) are intriguing to the scientific community due to their utility in wastewater treatment, electrosynthesis of biocommodities, and powering battery-less remote sensors. A model EAB, the electrode-respiring bacteria Geobacter sulfurreducens is able to directly transfer electrons to electrodes, resulting in high electron transfer efficiencies. The PI has experience in electrochemical techniques that can quantify the electron transfer efficiency in EABs and compare them to the model Geobacter sulfurreducens. This project, under the mentorship of Dr. Inseop Chang of the Gwangju Institute of Science and Technology (GIST) in South Korea, will quantify the electron transfer efficiency of thermophilic EABs that have been isolated by the host laboratory from distillery wastewater treatment microbial fuel cells. This collaboration will provide access to thermophiles that have only been observed by the host researcher?s laboratory.Many studies on EABs focus on the current density that was achieved in a controlled experiment, often treating the electron transfer process as a black box. This study will use the electrochemical quartz crystal microbalance employed in a bioelectrochemical system to evaluate the new strains performance as an electrochemically active biofilm, and compare it to model EABs at elevated temperatures. The working hypothesis is that thermophilic electrode-respiring bacteria isolated from distillery waste water microbial fuel cells will retain their current production capacities in monoculture and this can be quantified through the use of an electrochemical quartz crystal microbalance platform currently being developed at Washington State University. NSF EAPSI award is funded in collaboration with the National Research Foundation of Korea.

形成生物膜的产电菌之所以被称为产电菌，是因为它们能够与固体电极相互作用。这些电化学活性生物膜(EAB)因其在废水处理、生物商品的电合成和为无电池的远程传感器供电方面的应用而引起科学界的兴趣。作为一种模式EAB，电极呼吸细菌硫还原地杆菌能够直接将电子转移到电极上，从而产生高电子转移效率。PI在电化学技术方面有经验，可以量化EABs中的电子转移效率，并将其与模型硫磺还原菌进行比较。该项目在韩国光州科学技术研究所(GIST)的Inseop Chang博士的指导下，将量化由东道主实验室从酿酒废水处理微生物燃料电池中分离出的嗜热EAB的电子转移效率。这项合作将提供接触到只有主持研究人员S实验室才能观察到的嗜热菌。许多关于EAB的研究集中在受控实验中获得的电流密度上，通常将电子转移过程视为一个黑匣子。本研究将使用生物电化学系统中使用的电化学石英晶体微天平来评估新菌株作为电化学活性生物膜的性能，并将其与高温下的EABs模型进行比较。工作假设是，从酿酒废水中分离出的嗜热电极呼吸细菌微生物燃料电池将在单一培养中保持其现有的生产能力，这可以通过使用华盛顿州立大学目前正在开发的电化学石英晶体微天平平台来量化。NSF EAPSI奖是与韩国国家研究基金会合作资助的。