NSF-ANR: Cytochrome nanowires: secretion, assembly and function in ultrafast electron transfer by microbial biofilms

NSF-ANR：细胞色素纳米线：微生物生物膜超快电子转移的分泌、组装和功能

• 批准号: 2210473
• 负责人: Nikhil Malvankar
• 金额: $ 85万
• 依托单位: Yale University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-01-01 至 2025-12-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2210473&HistoricalAwards=false
• 关键词: NSF; ANR; Cytochrome; nanowires; secretion

Common soil bacterium Geobacter sulfurreducens has remarkable ability to form biofilms with high electronic conductivity rivalling those of synthetic polymers. This conductivity enables bacteria to transport respiratory electrons over hundreds of cell lengths to remote acceptors or partner cells. This process is critical for global environment and for applications in bioenergy, biofuels, and bioelectronics. It has been thought that biofilms transfer electrons via microbial nanowires made up of type IV pili filaments. The PI recently solved the first structure of a pilus from an organism capable of extracellular electron transfer (EET). These pili were thought to account for filament conductivity, but the work from the PI’s lab found that their conductivity is very low, in agreement with their structure. The aim of this project is to decipher the physical basis of nanowire secretion, assembly, and function. This project will characterize the G. sulfurreducens secretion machinery and the role of this novel class of bipartite pili. This project will identify the physical basis of pili -mediated cytochrome secretion and identify all factors essential for their biogenesis and develop tools to characterize the physical basis of nanowire secretion. This research will lead to a bioelectronic platform to manufacture pili required for the secretion of living biomaterials. This project will train users with different backgrounds in the use of multifunctional biomaterials to prepare the next generation of trans-disciplinary scientists trained at the interface of biology, physics, chemistry, data science and engineering.The primary aims of this project include reconstitution of G. sulfurreducens pili biogenesis, assembly, and secretion pathway in a heterologous system to dissect protein-protein interactions. Another aim is the elucidation of the mechanism of pilus dynamics and mechanical stability in the heterodimeric pilus assembly. The aims include determination of the mechanism of OmcS/Z secretion by quantifying how they interact with pili, and the characterization of the structure of the novel Geobacter pili-based cytochrome secretion system by cryo-electron tomography. This collaborative US/France project is supported by the US National Science Foundation and the French Agence Nationale de la Recherche, where NSF funds the US investigator and ANR funds the partners in France.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

常见的土壤细菌地球杆菌硫糖具有出色的能力，可以用高电子电导率撕裂合成聚合物的生物膜。该电导率使细菌能够将数百个细胞长度的呼吸电子传递到远程受体或伴侣细胞。这个过程对于全球环境和生物能源，生物燃料和生物电子学至关重要。人们认为，生物膜通过由IV型菌丝丝组成的微生物纳米线传输电子产品。 PI最近从能够细胞外电子转移（EET）的生物体中求解了吡喃的第一个结构。这些pili被认为是细丝电导率的解释，但是PI实验室的工作发现它们的电导率很低，与它们的结构一致。该项目的目的是破译纳米线分泌，组装和功能的物理基础。该项目将表征G.硫核的分泌机械以及这种新颖的双方菌毛的作用。该项目将确定PILI介导的细胞色素分泌的物理基础，并确定其生物发生和开发工具所必需的所有因素以表征纳米线分泌的物理基础。这项研究将导致一个生物电子平台，用于生产生物材料的分泌所需的pili。该项目将在使用多功能生物材料方面培训具有不同背景的用户，以准备在生物学，物理学，化学，数据科学和工程界面培训的下一代跨学科科学家。另一个目的是阐明异二聚体pyrus组装中的吡咯动力学机理和机械稳定性。目的包括通过量化它们与PILI的相互作用的方式来确定OMCS/Z分泌的机理，以及通过冷冻电子层析成像的新型Geobacter Pili的细胞色素分泌系统的结构来表征。美国国家科学基金会和法国Agence nationale de la recherche的支持，NSF为美国调查员提供资金，并在法国资助法国的合作伙伴资金。该奖项反映了NSF的法定任务，并通过评估该基金会的智力优先级和广泛的影响来评估CRITERIA。