Collaborative Research: Elucidating the Molecular Architecture and Dynamics of Phenylalanine Biosynthesis in Plants

合作研究:阐明植物中苯丙氨酸生物合成的分子结构和动力学

基本信息

项目摘要

The amino acid phenylalanine is the precursor for the synthesis of 8000 phenolic compounds in plants, which have profound impacts on plant growth, development, reproduction and defense. Phenylalanine serves as a precursor for numerous plant natural products that are widely used in medicine and as food supplements. This project will characterize a new pathway for phenylalanie synthesis in the plant cytostol determine the contribution of this pathway to the total phenylalanine production. The results will significantly contribute to the fundamental knowledge of a major plant metabolic network and provide a better understanding of aromatic amino acid biosynthesis. Such information will allow rational metabolic engineering of phenylalanine levels for production of important phenylalanine-derived compounds to improve agronomic traits, biofuel production, palatability of produce, and commercial value of ornamentals. The plannned research will provide multidisciplinary training to undergraduate and graduate students. The educational program will also introduce students from a local middle school to STEM-based research through direct classroom interactions and facilitating laboratory exercises where students generate and test hypotheses. It has long been accepted that aromatic amino acid biosynthesis occurs exclusively in plastids where it is subject to complex feedback regulation both at the entry point into the network and at the committed steps towards individual amino acids. The recent finding that phenylalanine is also formed in the cytosol raises new questions about how plants regulate carbon flux distribution between the parallel pathways. This project will employ an integrative strategy comprised of genetics, molecular biology, metabolic profiling, protein and membrane biochemistry, stable-isotope labeling, and metabolic flux analysis to determine if (i) plants contain a complete cytosolic pathway, (ii) this route is subject to less stringent feedback regulation, and (iii) the flux through this pathway increases when the demand for phenylalanine production and consumption increases. Petunia flowers, which are amenable to genetic alterations without deleterious effects to plant vitality, and are rich in phenylalanine and phenylalanine-derived volatiles, will be used as a model system. Specifically, this project will uncover the pathway structure and the molecular players of the cytosolic phenylalanine biosynthesis. Moreover, the project will reveal the capacity and relative contribution of the cytosolic route to overall production of phenylalanine.
苯丙氨酸是植物体内合成8000多种酚类化合物的前体,这些酚类化合物对植物的生长、发育、繁殖和防御有着深远的影响。苯丙氨酸是许多植物天然产物的前体,广泛用于医学和食品补充剂。本项目将描述植物细胞中苯丙氨酸合成的新途径,确定该途径对总苯丙氨酸产量的贡献。 这些结果将有助于对植物代谢网络的基础知识的了解,并为芳香族氨基酸的生物合成提供更好的理解。这样的信息将允许苯丙氨酸水平的合理代谢工程化,用于生产重要的苯丙氨酸衍生化合物,以改善农艺性状、生物燃料生产、产品的适口性和植物的商业价值。计划的研究将为本科生和研究生提供多学科培训。该教育计划还将通过直接的课堂互动和促进学生生成和测试假设的实验室练习,向当地中学的学生介绍STEM研究。芳香族氨基酸的生物合成仅发生在质体中,在质体中,芳香族氨基酸在进入网络的入口点和朝向单个氨基酸的承诺步骤处都受到复杂的反馈调节。最近发现苯丙氨酸也形成于胞质溶胶中,这就提出了关于植物如何调节平行途径之间碳通量分布的新问题。该项目将采用遗传学,分子生物学,代谢谱,蛋白质和膜生物化学,稳定同位素标记和代谢通量分析组成的综合策略,以确定(i)植物是否包含完整的胞质途径,(ii)该途径是否受到不太严格的反馈调节,以及(iii)当苯丙氨酸生产和消费需求增加时,通过该途径的通量增加。矮牵牛花,这是经得起遗传改变而不会对植物活力产生有害影响,并富含苯丙氨酸和苯丙氨酸衍生的挥发物,将被用作一个模型系统。具体而言,本项目将揭示细胞溶质苯丙氨酸生物合成的途径结构和分子参与者。此外,该项目还将揭示胞质途径对苯丙氨酸总产量的能力和相对贡献。

项目成果

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Guillaume Pilot其他文献

Elicitors of plant defense induce the accumulation of amino acids that suppress both bacterial virulence and growth
植物防御诱导子诱导氨基酸积累,抑制细菌毒力和生长
  • DOI:
  • 发表时间:
    2022
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Xiaomu Zhang;Philip J. Tubergen;Israel D K Agorsor;Pramod Khadka;Connor Tempe;Eva Collakova;Guillaume Pilot;C. Danna
  • 通讯作者:
    C. Danna
transporter activity Overexpression of stomatin depresses GLUT-1 glucose
转运蛋白活性 stomatin 的过度表达会抑制 GLUT-1 葡萄糖
  • DOI:
  • 发表时间:
    2011
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Wafa Abbud;R. Prohaska;F. Ismail;Guillaume Pilot;D. Bushey;Réjane Pratelli;Glutamine Dumper;M. Sitbon;N. Taylor;A. Montel;L. Blanc;Myriam Boyer;C. Jacquet;M. Vidal;Mario Mairhofer;Marianne Steiner;U. Salzer;S. Gospe;S. Baker;V. Arshavsky
  • 通讯作者:
    V. Arshavsky
Induction of GLUTAMINE DUMPER1 reveals a link between amino acid export, abscisic acid, and immune responses
GLUTAMINE DUMPER1 的诱导揭示了氨基酸输出、脱落酸和免疫反应之间的联系
  • DOI:
  • 发表时间:
    2020
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Shi Yu;Delasa Aghamirzaie;K. Harich;Eva Collakova;R. Grene;Guillaume Pilot
  • 通讯作者:
    Guillaume Pilot
Mining for meaning: visualization approaches to deciphering Arabidopsis stress responses in roots and shoots.
挖掘意义:破译拟南芥根和芽应激反应的可视化方法。
  • DOI:
    10.1089/omi.2011.0111
  • 发表时间:
    2012
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Lecong Zhou;C. Franck;Kuan Yang;Guillaume Pilot;L. Heath;R. Grene
  • 通讯作者:
    R. Grene
Suppressor mutations in the Glutamine Dumper1 protein dissociate disturbance in amino acid transport from other characteristics of the Gdu1D phenotype
谷氨酰胺转储器 1 蛋白的抑制突变将氨基酸转运的干扰与 Gdu1D 表型的其他特征分离
  • DOI:
  • 发表时间:
    2015
  • 期刊:
  • 影响因子:
    5.6
  • 作者:
    Shi Yu;Réjane Pratelli;C. Denbow;Guillaume Pilot
  • 通讯作者:
    Guillaume Pilot

Guillaume Pilot的其他文献

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{{ truncateString('Guillaume Pilot', 18)}}的其他基金

Conference: 7th Pan American Plant Membrane Biology Workshop, Merida, Mexico, June 18-21 2023
会议:第七届泛美植物膜生物学研讨会,墨西哥梅里达,2023 年 6 月 18-21 日
  • 批准号:
    2328521
  • 财政年份:
    2023
  • 资助金额:
    $ 27.5万
  • 项目类别:
    Standard Grant
Mechanisms of nutrient transport from plants to biotrophic pathogens
养分从植物到生物营养病原体的运输机制
  • 批准号:
    1353366
  • 财政年份:
    2014
  • 资助金额:
    $ 27.5万
  • 项目类别:
    Continuing Grant

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