SCH: INT: Disposable High Sensitivity Point of Care Immunosensors for Multiple Disease and Pathogen Detection

这项研究计划建议调查汗液中有时发现的物质，如蛋白质，它可以检测健康和疾病(生物标记物)。汗液是一种生物流体，可以为一个人的健康提供一个窗口。目前，还没有快速的方法来测量汗液中发出疾病信号的蛋白质的变化。其目标是测量体力活动之前、期间和之后汗液中这些蛋白质的变化，包括使用低成本和一次性护理点生物传感器测量应激激素和免疫标记物的水平。本质上，这项提议的目的是开发一种廉价的一次性贴片传感器，可以佩戴在皮肤上，最终成为像大多数家庭药柜中的绷带盒或口腔温度计一样广泛使用的传感器。这种设备的关键工程障碍之一是能否获得一种低成本的制造技术，该技术可以与最先进的生物标志物蛋白质组检测技术相结合，以医学实验室级别的灵敏度诊断和监测多种疾病。这项建议提出了一种新的方法，将低成本的商业显示技术(在您的电视、计算机显示器或手机上可以找到)与蛋白质微阵列打印技术相结合，以制造低成本的一次性皮肤贴片，以便在家庭或其他非临床环境中更有效地自我管理患者的健康护理。患者和他们的照顾者都可以使用智能手机技术实时进行测量。为了满足这一研究目标，提出了一种类似织物的柔性皮肤贴片，它将提供对人体汗液中多个生物标志物的非侵入性监测。这一新方法旨在利用现有的商业制造技术，这些技术可以很容易地改装成每年以几美元的成本生产数亿台设备。研究计划建议利用多个蛋白质组研究平台(质谱学、蛋白质和抗体微阵列)来建立汗液蛋白质组的图谱，这将对加深对汗液和皮肤的生物学理解产生根本影响，并将高成本的基于荧光的分析平台转变为低成本、一次性、高灵敏度的基于荧光护理点的分析方法，该分析方法最小限度地到非侵入性的。拟议开发工作的重点包括：利用质谱仪以及定制的蛋白质和抗体微阵列进行详细的蛋白质组分析，以发现汗液中存在的蛋白质和抗体；开发和优化生物传感器的光学和电子元件，用于低成本的荧光识别横向流动分析；利用已识别的血清和汗液生物标志物开发荧光识别横向流动分析；以及将该分析与我们的光学生物传感器集成。这项研究计划加快了技术的发展，将医疗保健从反应性和医院中心转变为预防性、主动性、循证和以人为中心。这一新一代医疗保健解决方案将为家庭监控提供突破性的传感器技术。医疗保健领域的这种转变对免疫功能受损的个人尤其关键，例如器官移植接受者、癌症患者、术后患者和老年人。

This research program proposes to investigate substances sometimes found in sweat, such as protein, which can detect health and disease (biomarkers). The sweat is a biofluid that can provide a window to a person's health. At this time, there is no rapid method to measure the changes in proteins in the sweat that signal disease. The goal is to measure changes in these proteins in the sweat before, during, and after physical activity, including measuring levels of stress hormones and immune markers using a low cost and disposable point-of-care biosensor. In essence, the aim of this proposal is to develop a cheap and disposable patch sensor that can be worn on the skin, and eventually become as widely used as the box of bandages or oral thermometer found in most home medicine cabinets. One of the key engineering roadblocks to such a device is the availability of a low-cost manufacturing technology that can be combined with state-of-the-art biomarker proteomic detection technology to diagnose and monitor multiple diseases with medical laboratory level sensitivity. This proposal presents a new approach combining low-cost commercial display technology (found in your TV, computer monitor, or cell phone) with protein microarray printing technology to fabricate a low-cost, disposable skin patch for more effective self-management of patient health care in the home or in other non-clinical settings. The measurements will be available in real time for both patients and their caregivers using smart-phone technology. To meet this research objective, a fabric-like flexible skin patch is proposed that would provide non-invasive monitoring of multiple biomarkers in human sweat. This new approach is designed to use existing commercial manufacturing technology that can be readily adapted to produce hundreds of millions of devices per year at a cost of only a few dollars each.The research program proposes to utilize multiple proteomic research platforms (mass spectrometry, protein, and antibody microarrays) to establish a profile of the sweat proteome that will have a fundamental impact on furthering the biological understanding of sweat and skin, and transition high-cost fluorescent-based assay platforms into a low-cost, disposable, highly sensitive fluorescent point-of-care based assay that is minimally to non-invasive. Highlights of the proposed development effort include: a detailed proteomic analysis utilizing mass spectrometry as well as custom protein and antibody microarrays for the discovery of proteins and antibodies present in sweat; development and optimization of the biosensor's optical and electronic components for the utilization of low-cost fluorescent recognition lateral flow assay; development of fluorescent recognition lateral flow assays utilizing identified serum and sweat biomarkers and the integration of the assay with our optical biosensor. This research program accelerates the development of technology that will transform healthcare from reactive and hospital-centered to preventative, proactive, evidence-based, and person-centered. This next generation health care solution will provide breakthrough sensor technology for in-home monitoring. This transformation in healthcare is especially critical for immune-compromised individuals, such as organ transplant recipients, cancer patients, post-operative patients, and the elderly.