SCH: INT: Disposable High Sensitivity Point of Care Immunosensors for Multiple Disease and Pathogen Detection

SCH：INT：用于多种疾病和病原体检测的一次性高灵敏度护理点免疫传感器

• 批准号: 1521904
• 负责人: Jennifer Blain Christen
• 金额: $ 182.44万
• 依托单位: Arizona State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-08-01 至 2021-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1521904&HistoricalAwards=false
• 关键词: SCH; INT; Disposable; Sensitivity; Point

This research program proposes to investigate substances sometimes found in sweat, such as protein, which can detect health and disease (biomarkers). The sweat is a biofluid that can provide a window to a person's health. At this time, there is no rapid method to measure the changes in proteins in the sweat that signal disease. The goal is to measure changes in these proteins in the sweat before, during, and after physical activity, including measuring levels of stress hormones and immune markers using a low cost and disposable point-of-care biosensor. In essence, the aim of this proposal is to develop a cheap and disposable patch sensor that can be worn on the skin, and eventually become as widely used as the box of bandages or oral thermometer found in most home medicine cabinets. One of the key engineering roadblocks to such a device is the availability of a low-cost manufacturing technology that can be combined with state-of-the-art biomarker proteomic detection technology to diagnose and monitor multiple diseases with medical laboratory level sensitivity. This proposal presents a new approach combining low-cost commercial display technology (found in your TV, computer monitor, or cell phone) with protein microarray printing technology to fabricate a low-cost, disposable skin patch for more effective self-management of patient health care in the home or in other non-clinical settings. The measurements will be available in real time for both patients and their caregivers using smart-phone technology. To meet this research objective, a fabric-like flexible skin patch is proposed that would provide non-invasive monitoring of multiple biomarkers in human sweat. This new approach is designed to use existing commercial manufacturing technology that can be readily adapted to produce hundreds of millions of devices per year at a cost of only a few dollars each.The research program proposes to utilize multiple proteomic research platforms (mass spectrometry, protein, and antibody microarrays) to establish a profile of the sweat proteome that will have a fundamental impact on furthering the biological understanding of sweat and skin, and transition high-cost fluorescent-based assay platforms into a low-cost, disposable, highly sensitive fluorescent point-of-care based assay that is minimally to non-invasive. Highlights of the proposed development effort include: a detailed proteomic analysis utilizing mass spectrometry as well as custom protein and antibody microarrays for the discovery of proteins and antibodies present in sweat; development and optimization of the biosensor's optical and electronic components for the utilization of low-cost fluorescent recognition lateral flow assay; development of fluorescent recognition lateral flow assays utilizing identified serum and sweat biomarkers and the integration of the assay with our optical biosensor. This research program accelerates the development of technology that will transform healthcare from reactive and hospital-centered to preventative, proactive, evidence-based, and person-centered. This next generation health care solution will provide breakthrough sensor technology for in-home monitoring. This transformation in healthcare is especially critical for immune-compromised individuals, such as organ transplant recipients, cancer patients, post-operative patients, and the elderly.

该研究计划提出调查有时在汗液中发现的物质，如蛋白质，可以检测健康和疾病（生物标志物）。汗液是一种生物流体，可以为一个人的健康提供一个窗口。目前，还没有快速的方法来测量汗液中蛋白质的变化，这些变化是疾病的信号。其目标是测量身体活动之前，期间和之后汗液中这些蛋白质的变化，包括使用低成本和一次性护理点生物传感器测量应激激素和免疫标记物的水平。从本质上讲，这项提案的目的是开发一种廉价的一次性贴片传感器，可以戴在皮肤上，并最终成为广泛使用的绷带盒或口腔温度计在大多数家庭药柜中发现。这种设备的关键工程障碍之一是低成本制造技术的可用性，该技术可以与最先进的生物标志物蛋白质组检测技术相结合，以医学实验室水平的灵敏度诊断和监测多种疾病。该提案提出了一种新的方法，将低成本的商业显示技术（在电视，计算机显示器或手机中发现）与蛋白质微阵列打印技术相结合，以制造低成本的一次性皮肤贴片，用于在家庭或其他非临床环境中更有效地自我管理患者的健康护理。这些测量结果将通过智能手机技术为患者及其护理人员提供真实的实时信息。 为了实现这一研究目标，提出了一种织物状柔性皮肤贴片，其将提供对人类汗液中的多种生物标志物的非侵入性监测。这种新方法旨在利用现有的商业制造技术，这些技术可以很容易地进行调整，以每年生产数亿台设备，每台设备的成本仅为几美元，该研究计划提出利用多个蛋白质组学研究平台（质谱，蛋白质，和抗体微阵列）为了建立汗液蛋白质组的概况，这将对进一步了解汗液和皮肤的生物学产生根本性的影响，并将高成本的基于荧光的测定平台转变为低成本的、一次性的、高灵敏度的基于护理点的荧光的测定，其是最低限度的至非侵入性的。拟议开发工作的重点包括：利用质谱法进行详细的蛋白质组学分析以及定制蛋白质和抗体微阵列，以发现汗液中存在的蛋白质和抗体;开发和优化生物传感器的光学和电子组件，以利用低成本荧光识别侧流分析;开发了利用已鉴定的血清和汗液生物标志物的荧光识别侧向流测定法，并将该测定法与我们的光学生物传感器整合。该研究计划加速了技术的发展，将医疗保健从反应性和以医院为中心转变为预防性，主动性，循证和以人为本。这种下一代医疗保健解决方案将为家庭监控提供突破性的传感器技术。医疗保健的这种转变对于免疫受损的个体尤其重要，例如器官移植受者，癌症患者，术后患者和老年人。