MRI: Acquisition of High performance Liquid Chromatography and Mass Spectrometry Instrumentation to Support Research and Training at the University of Puget Sound

通过主要研究仪器（MRI）和化学研究仪器和设施（CRIF）项目的资助，普吉特海湾大学将获得一套液相色谱质谱计系统（LC/MS）。该系统将用于分析从各种来源获得的样品的组成，包括从环境和生物来源获得的样品。液相色谱法（LC）将混合物分离成其单独的成分。质谱法（MS）将这些成分电离，并通过测量离子的质量电荷比（m/z）来确定它们的质量。这是一种广泛使用的分析工具，用于确定混合物或材料的成分。该仪器将用于学生的研究，训练他们使用先进的现代仪器。在实验课程中，学生将学习如何使用它。除了对普吉特海湾的科学课程产生影响外，该仪器还将在普吉特海湾与塔科马科学与数学研究所（SAMI）（一所磁铁STEM高中）之间的计划合作中发挥核心作用。该奖项以环境和生物化学研究为重点。该提案旨在加强研究，特别是在以下领域：(a)探测暴露于抗菌肽的生物膜中脂质表达的变化；(b)量化废水中的非法药物和药物代谢物；(c)开展植物光敏色素反应的转录组学和蛋白质组学比较分析；(d)研究双酚A在调节生殖神经内分泌系统中的作用；(e)评估普吉特海湾废弃锯木厂场址的营养水平与持久性有机污染物生物积累之间的相关性。

With this award from the Major Research Instrumentation (MRI) and Chemistry Research Instrumentation and Facilities (CRIF) programs, the University of Puget Sound will acquire a liquid chromatography mass spectrometer system (LC/MS). The system will be used to analyze the composition of samples obtained from various sources including those obtained from environmental and biological origins. Liquid chromatography (LC) separates a mixture into its individual components. Mass spectrometry (MS) then ionizes the components and determines their mass by measuring the mass to charge ratio (m/z) of the ions. This is a widely used analytical tool to identify what is the composition of a mixture or material. The instrument will be used by students in their research, training them with sophisticated, modern instrumentation. Students in laboratory courses will be trained in its use. In addition to its impact on the scientific curriculum at Puget Sound, the instrument will play a central role in a planned collaboration between Puget Sound and the Tacoma Science and Math Institute (SAMI), a magnet STEM high school.This award has a environmental and biochemical research focus. The proposal is aimed at enhancing research especially in areas such as (a) probing changes in lipid expression in biofilms exposed to antimicrobial peptides; (b) quantifying illicit drugs and pharmaceutical metabolites in wastewater; (c) carrying out comparative transcriptomic and proteomic analysis of phytochrome responses in plants; (d) examining the role of bisphenol A in the modulation of the reproductive neuroendocrine system; and (e) assessing the correlation between trophic level and bioaccumulation of persistent organic pollutants at abandoned sawmill sites in the Puget Sound.