MRI: Acquisition of a Cryo Field Emission Transmission Electron Microscope

MRI：购买冷冻场发射透射电子显微镜

• 批准号: 1531757
• 负责人: Susan Anagnost
• 金额: $ 112万
• 依托单位: SUNY College of Environmental Science and Forestry
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-09-01 至 2018-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1531757&HistoricalAwards=false
• 关键词: MRI; Acquisition; Cryo; Field; Emission

An award is made to State University of New York College of Environmental Science and Forestry (ESF) to acquire a field emission scanning/transmission electron microscope (FES/TEM) with cryo-capabilities and elemental analysis using Energy Dispersive Spectroscopy (EDS). This instrument will replace a 30 year-old failing TEM in the shared-core N.C. Brown Center for Ultrastructure Studies at ESF. This project is a joint partnership of three adjacent universities, ESF, SUNY Upstate Medical University (UMU) and Syracuse University (SU). This new FES/TEM will provide these institutions with capabilities that are currently not available in central New York, and will expand the research capabilities of faculty and graduate students at these institutions, and provide support for competitive extramural funding. The NC Brown Center at ESF offers a unique academic program, a "Microscopy Minor" in central New York with graduate and undergraduate coursework and comprehensive formal training in the theory and application of microscopy such as: sample preparation, instrumentation and interpretation of results. This acquisition will enhance these academic offerings. In addition to its academic program, the Center routinely provides light, scanning and transmission microscopy demonstrations to community and industrial groups including outreach activities and demonstrations. This project will support such activities. The new FES/TEM will facilitate interactive, online high school and client access, with potential to provide interactive information with online demos. The new TEM enables the asbestos testing lab at ESF to become the only local laboratory to offer both phase contrast and TEM asbestos analysis for the asbestos remediation industry. Societal benefits of the project include raising scientific literacy of students and the public, providing students with skills for employment, providing potential for research that can result in wide-ranging impacts such as new vaccines or drug delivery systems for disease prevention, as well as environmental and industrial impact from the development of novel nanomaterials.Acquisition of this cryo-capable field emission scanning and transmission (S/TEM) will permit advances within a wide-range of research groups at ESF, SU and UMU. Among these, at ESF: better tracking of measurable chemical changes and ultrastructure in fish ear stones due to environmental conditions, chemistry of both inorganic and organic nanoparticles, with potential use in targeted drug delivery, studies of wood cell wall degradation by fungi affecting forest trees and wood products, studies of insect vectors of human and plant diseases; at SU: this microscope will determine size and shape of semi-conductive quantum rods and alloy nanoparticles, atomic imaging and elemental inspection of atomic derived lattice planes and compositional gradients in alloy nanoparticles, S/TEM and EDS analysis of compositional changes in hetero-structured nanoparticles, S/TEM of stacking faults and extended defects in quantum dots, and analysis of protein and DNA modified nanomaterials; and at UMU: the proposed field emission electron source with its superior brilliance and beam coherence will produce high contrast images from frozen hydrated biological macromolecules at much better resolution than is now possible with the older machine. Such data will allow 3-D structure determination of ATP molecular motors. The high resolution 4K CCD camera will enable high throughput situations especially when thousands of images are collected for single particle or 3Dimensional reconstruction. The motorized goniometer will enable generating tomographic or tilted reconstructions of cryo sections of animal or plant cells revealing the internal structure and location of organelles, virus or nanoparticles as well as large drug molecules without the confusing artifacts created by chemical fixation. This microscope features a 2 nanometer resolution for examination of unstained samples, bacteria, viruses, proteins, nanoparticles and at the same time, map and locate elements. This new generation S/TEM will have a field emission gun, cryo-capable stage, Energy Dispersive X-ray Spectrometer, 4K CCD digital camera, electron diffraction, tomography software, and remote access. This instrument permits entry into the fields of protein folding, molecular motors, materials research, 3D reconstruction of single particles, cryo-imaging and elemental analysis. The FES/TEM with these options will enable visualization and reconstruction of subcellular particles, drug delivery vehicles, identification of elements in biological samples and nanoparticles, and localization of pharmaceuticals in target tissues by cryo-sections; all things that cannot be achieved with present instrumentation.

授予州立大学纽约环境科学和林业学院一项合同，以获得一台具有低温能力的场发射扫描/透射电子显微镜，并使用能量色散光谱法进行元素分析。 这台仪器将取代一台有30年历史的故障TEM。ESF布朗超微结构研究中心 该项目是三个相邻的大学，ESF，纽约州立大学北部医科大学（UMU）和锡拉丘兹大学（SU）的联合伙伴关系。 这一新的FES/TEM将为这些机构提供目前纽约中心所没有的能力，并将扩大这些机构的教师和研究生的研究能力，并为竞争性校外资金提供支持。 在ESF的NC布朗中心提供了一个独特的学术课程，在纽约市中心的“显微镜未成年人”与研究生和本科课程和全面的正规培训的理论和应用显微镜，如：样品制备，仪器和结果的解释。 此次收购将加强这些学术产品。 除了其学术计划，该中心定期提供光，扫描和透射显微镜演示社区和工业团体，包括推广活动和示范。该项目将支持这些活动。 新的FES/TEM将促进交互式的在线高中和客户访问，并有可能通过在线演示提供交互式信息。新的TEM使ESF的石棉测试实验室成为当地唯一一家为石棉修复行业提供相位对比和TEM石棉分析的实验室。 该项目的社会效益包括提高学生和公众的科学素养，为学生提供就业技能，为可能产生广泛影响的研究提供潜力，如预防疾病的新疫苗或药物输送系统，以及新型纳米材料的发展对环境和工业的影响。获得这种具有低温能力的场发射扫描和透射（S/TEM）将允许在ESF，SU和UMU的广泛研究小组内的进步。 其中，在ESF：由于环境条件，无机和有机纳米颗粒的化学性质，在靶向药物递送中具有潜在用途，影响森林树木和木制品的真菌对木材细胞壁降解的研究，人类和植物疾病昆虫媒介的研究，更好地跟踪鱼耳石中可测量的化学变化和超微结构;在SU：该显微镜将确定半导体量子棒和合金纳米颗粒的尺寸和形状，原子衍生的晶格平面的原子成像和元素检查以及合金纳米颗粒中的成分梯度，异质结构纳米颗粒中成分变化的S/TEM和EDS分析，量子点中堆垛层错和扩展缺陷的S/TEM分析，以及蛋白质和DNA修饰纳米材料的分析;在UMU：所提出的场发射电子源具有上级亮度和束相干性，将从冷冻水合生物大分子产生高对比度图像，其分辨率比现在好得多可能是旧机器。这些数据将允许ATP分子马达的3-D结构确定。 高分辨率4K CCD摄像机将实现高通量的情况下，特别是当数以千计的图像收集单个颗粒或三维重建。 电动测角仪将能够生成动物或植物细胞冷冻切片的断层扫描或倾斜重建，揭示细胞器，病毒或纳米颗粒以及大药物分子的内部结构和位置，而不会产生化学固定造成的混乱工件。该显微镜具有2纳米分辨率，可用于检查未染色的样品，细菌，病毒，蛋白质，纳米颗粒，同时绘制和定位元素。这种新一代S/TEM将具有场发射枪、低温台、能量色散X射线光谱仪、4K CCD数码相机、电子衍射、层析成像软件和远程访问。 该仪器允许进入蛋白质折叠，分子马达，材料研究，单个粒子的3D重建，冷冻成像和元素分析等领域。 具有这些选项的FES/TEM将使亚细胞颗粒、药物递送载体的可视化和重建、生物样品和纳米颗粒中的元素的鉴定以及通过冷冻切片在靶组织中的药物定位成为可能;所有这些都是目前仪器无法实现的。