ACQUISITION OF HIGH-THROUGHPUT 200 kV CRYO-TEM

获取高通量 200 kV 冷冻透射电镜

• 批准号: 10415650
• 负责人: Irina I Serysheva
• 金额: $ 200万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-07-01 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10415650
• 关键词: Area; Bacterial Drug Resistance; Cell membrane; Collaborations; Communicable Diseases; Computer software; Contract Services; Core Facility; Cost Sharing; Cryoelectron Microscopy; Electrons; Equipment; Fostering; Funding; Generations; Health Sciences; Image; Institution; Malignant Neoplasms; Medical center; Microscope; Neurosciences; Productivity; Research; Research Personnel; Research Project Grants; Resources; Sampling; Scientist; Specimen; Students; Texas; Time; Titan; Transmembrane Transport; United States National Institutes of Health; Universities; cost; design; detector; drug discovery; high resolution imaging; imaging facilities; instrument; medical schools; screening; structural biology; transmission process

Project Summary/Abstract The proposal is to request support for the purchase of the 200 kV Glacios Transmission Electron Cryo- microscope from Thermo Fisher Scientific Inc.. The requested instrument will replace our current Tecnai G2 Polara (300 kV) cryomicroscope, which was purchased nearly two decades ago and is currently at the end of its usefulness. At this time, Thermo Fisher does not provide service contract for the Polara and to keep the microscope operational is becoming a challenge that jeopardizes our investigators and their NIH funded projects. Strong institutional support is provided with a cost-sharing commitment of $1,000,000 to the purchase of the proposed Glacios and another ~$1,000,000 for over five years to support the projected operational costs of the new cryo-TEM. The requested instrument will be integrated within the Cryo-EM Core Facility established under the umbrella of the Structural Biology Imaging Center at McGovern Medical School at the University of Texas Health Science Center (UTHealth) at Houston. UTHealth’s Cryo-EM Core Facility is a well administered, highly collaborative and user focused cryo-EM resource currently equipped with a Tecnai G2 Polara and a Titan Krios G3. A Glacios is a new generation microscope offered by Thermo Fisher that will replace our obsolete Polara while at the same time offering significant technological improvements and enabling new capabilities for our users. That includes high-throughput specimen optimization and screening through the integrated EPU software, which can be followed by contamination-free sample transfer to the Titan Krios for high-resolution imaging; optimized cryo-EM imaging using the latest generation of electron counting detector Falcon 4 integrated with the new Selectris energy filter, designed for stability and ease-of-use. The proposed Glacios will serve the needs of over two dozen research projects in key areas such as cancer, neuroscience, infectious diseases, drug discovery, antibacterial drug resistance, cell membrane transport, and others. It will deeply impact the research in the Texas Medical Center and surrounding institutions by: increasing scientific productivity; fostering interactions and collaborations amongst scientists, strengthening the competitiveness for research funding, and boosting our ability to attract new investigators and students by the prospect of using start-of-the-art cryo-EM equipment.

项目摘要/摘要 该提案是请求支持购买200千伏冰川传输电子冷冻机- 来自Thermo Fisher Science Inc.的显微镜。所要求的仪器将取代我们目前的Tecnai G2 Polara(300千伏)冷冻显微镜，它是近20年前购买的，目前处于 它的用处。目前，Thermo Fisher没有为Polara提供服务合同，并保留了 显微镜操作正在成为一项挑战，危及我们的研究人员和他们的NIH资助 项目。提供了强有力的机构支持，并为购买提供了100万美元的成本分担承诺 和另外1,000,000美元，用于五年以上，以支持预计的运营成本 新的低温透射电子显微镜。所要求的仪器将整合到已建立的低温电磁核心设施中 在华盛顿大学麦戈文医学院结构生物学成像中心的保护伞下 休斯顿的德克萨斯健康科学中心(UTHealth)。UTHealth的冷冻-EM核心设施管理良好， 高度协作且以用户为中心的低温电磁资源目前配备了Tecnai G2 Polara和 泰坦·克里奥斯G3。冰川是Thermo Fisher提供的新一代显微镜，它将取代我们的 过时的Polara，同时提供显著的技术改进并实现新的 为我们的用户提供功能。这包括高通量样本优化和通过 集成的EPU软件，随后可以将无污染的样本传输到泰坦·克里奥斯，用于 高分辨率成像；使用最新一代电子计数探测器优化的低温电磁成像 Falcon 4集成了新的SElectris能量过滤器，设计稳定且易于使用。建议数 Glacios将服务于癌症、神经科学、 传染病、药物发现、抗菌药物耐药性、细胞膜转运等。会的 通过以下方式深刻影响德克萨斯医学中心和周边机构的研究：增加科学研究 生产力；促进科学家之间的互动和合作，增强 研究资金，并通过使用前景来增强我们吸引新研究人员和学生的能力 最先进的低温电磁设备。