Development and microfluidic characterisation of a dynamically cultivated full skin model

该建议是基于联合项目-微流体动态培养全层皮肤模型的开发和表征-。该项目的目的是在动态培养条件下开发人体皮肤模型，并在物质运输、组织形成和微流体方面对其进行表征。它是基于多器官芯片（MOC）作为一种在芯片上对人体器官进行建模的新技术。这为生理近似皮肤等价物的发展提供了一个独特的平台。通过适应性强的构建方法和快速的设计周期，可以逐步开发模块化的皮肤模型，包括脂肪组织，毛囊或血管系统等组件。在目前的项目中，这种皮肤模型得到了成功的开发，并详细阐述了用于模拟质量运输和分布效应的渗透系数，这些效应与营养物质和细胞成分的供应和处置中产生的代谢物以及大分子成分有关。到目前为止，重点是了解有效成分的渗透，在后续项目中，将研究工业制造的纳米材料在人体皮肤模型中的渗透和渗透。为此，将建立新的、有意义的研究方法来检测纳米材料在人体皮肤中的渗透和渗透。柏林工业大学开发的基于芯片的长期皮肤培养技术将通过体外和体内数据与其他方法相比，对纳米材料暴露进行优化。

The proposal is based on the joint project -Development and characterization of a microfluidic dynamically cultured full-thickness skin model-. The aim of the project is the development of human skin models in dynamic culture conditions as well as their characterization with regard to substance transport, tissue formation and microfluidic. It is based on a multi-organ chip (MOC) as a new technology for modeling of segments of the human organism on a chip. This offers a unique platform for the development of physiologically approximated skin equivalents. By the adaptable construction method and quick design cycles, modular skin models can be developed stepwise including components like fatty tissue, hair follicle or a vascular system. In the current project this skin models were successfully developed and permeability coefficients for the simulation of mass transport and distribution effects with respect to the nutrients and the resulting metabolites in the supply and disposal of cell components as well as for large molecule ingredients were elaborated. Whereas so far the focus was on understanding the penetration of active ingredients, in the follow-up project penetration and permeation of industrially manufactured nanomaterials in human skin models will be studied. For this, new and meaningful study methods for the detection of penetration and permeation of nanomaterials in and through human skin will be established. The chip-based long term skin culture technology developed at TU Berlin will be optimized with respect to nanomaterial exposure and compared to other methods by in vitro and in vivo data.