CAREER: High-throughput Imaging of Drug Response in Tumor Organoids

职业：肿瘤类器官药物反应的高通量成像

• 批准号: 1554027
• 负责人: Melissa Skala
• 金额: $ 50.95万
• 依托单位: Vanderbilt University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-05-01 至 2016-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1554027&HistoricalAwards=false
• 关键词: CAREER; throughput; Imaging; Drug; Response

ABSTRACTPI: Scala, Melissa Proposal: 1554027The proposal focuses on developing a high-throughput imaging technology to measure drug response and cellular metabolism in tumor cells cultured with cells in their native environment. The data obtained will be used to predict tumor response to multiple drugs, providing information that can be used to optimize drug treatment for the individual cancer patient. The Educational Plan, which includes a new undergraduate course in optical imaging, has a strong emphasis on outreach activities directed at middle and high school students and girls.Interference light sheet Fourier Fluorescence lifetime imaging microscopy (iLS-FP-FLIM) will be created and information obtained by imaging NADH and FAD coenzymes in human breast cancer cells grown in a mouse model. This will be used to predict tumor response to multiple drugs, providing information that can be used to optimize drug treatment for the individual cancer patient.

摘要/ abstract摘要：Scala， Melissa提案：1554027该提案的重点是开发一种高通量成像技术来测量肿瘤细胞在天然环境中培养的药物反应和细胞代谢。获得的数据将用于预测肿瘤对多种药物的反应，提供可用于优化单个癌症患者药物治疗的信息。《教育计划》包括开设一门新的光学成像本科课程，特别强调针对初高中学生和女孩的外联活动。将建立干涉光片傅立叶荧光寿命成像显微镜（iLS-FP-FLIM），并通过在小鼠模型中生长的人乳腺癌细胞中对NADH和FAD辅酶进行成像获得信息。这将用于预测肿瘤对多种药物的反应，提供可用于优化单个癌症患者药物治疗的信息。