CAREER: High-throughput Imaging of Drug Response in Tumor Organoids

Scala，Melissa提案：1554027该提案的重点是开发一种高通量成像技术，以测量与其自然环境中的细胞一起培养的肿瘤细胞的药物反应和细胞代谢。获得的数据将用于预测肿瘤对多种药物的反应，提供可用于优化个别癌症患者药物治疗的信息。该教育计划包括一门新的光学成像本科课程，重点是针对初中生和女孩的推广活动。将创建干涉光片傅立叶荧光寿命成像显微镜(ILS-FP-FLIM)，并通过成像在小鼠模型中培养的人乳腺癌细胞中的NADH和FAD辅酶来获得信息。这将被用来预测肿瘤对多种药物的反应，提供可用于为个别癌症患者优化药物治疗的信息。

ABSTRACTPI: Scala, Melissa Proposal: 1554027The proposal focuses on developing a high-throughput imaging technology to measure drug response and cellular metabolism in tumor cells cultured with cells in their native environment. The data obtained will be used to predict tumor response to multiple drugs, providing information that can be used to optimize drug treatment for the individual cancer patient. The Educational Plan, which includes a new undergraduate course in optical imaging, has a strong emphasis on outreach activities directed at middle and high school students and girls.Interference light sheet Fourier Fluorescence lifetime imaging microscopy (iLS-FP-FLIM) will be created and information obtained by imaging NADH and FAD coenzymes in human breast cancer cells grown in a mouse model. This will be used to predict tumor response to multiple drugs, providing information that can be used to optimize drug treatment for the individual cancer patient.