权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Importance of Acclimatory and Adaptive Response to the Environment

对环境的适应和适应性反应的重要性

基本信息

批准号：
1556396
负责人：
Marjorie Oleksiak
金额：
$ 72.35万
依托单位：
University of Miami
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
2017
资助国家：
美国
起止时间：
2017-04-15 至 2023-03-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1556396&HistoricalAwards=false
关键词：
Importance Acclimatory Adaptive Response Environment

项目摘要

Part 1: How animals survive and prosper under changing environmental conditions depends on both their physiological responses and adaptive changes in their genes. Physiological responses include changes in the metabolic rates and the proteins that affect metabolism during the animal's lifetime. These reversible, non-genetic changes buffer an individual's health in a changing environment. Yet, there are limits to these physiological responses, and animals may evolve genetic changes that affect their metabolism and enhance their survival. This research investigates the interaction between physiological and adaptive changes to better understand the mechanisms affecting health and fitness. This research takes advantage of new DNA sequencing technologies to define 10,000s of genetic differences among environments and relate these to measures of gene expression. The data from this research will inform society about the genes and gene expression that enhances health in a variety of environments. Included in this research is the training of undergraduate and high-school students from a diversity of economic and social backgrounds. Part 2:To enhance our ability to predict responses to climate change, we need to understand the relative importance of acclimatory responses and the potential for adaptive divergence. Although there are several good vertebrate models for thermal adaptive divergence among taxa and many acclimation response examples, few studies examine the interaction between acclimatory responses and adaptive divergence. To examine this interaction, this research first measures derived changes in metabolism and the effect of thermal acclimation and then uses the variation in these measures to interrogate the importance of mRNA and DNA variation. Two major goals of this proposed research are to utilize genotyping by sequencing to provide tens of thousands of single nucleotide polymorphisms and apply RNA-Seq to measure most expressed genes to determine if 1) nucleotide divergence among the three thermal effluent populations share similar adaptive divergence and 2) acclimatory and adaptive changes in mRNA expression affect the same loci. Our predictions are that acclimatory and adaptive changes in mRNA expression involve different genes, that many loci can effect a change in metabolism and thus different populations will have different mRNA expression patterns that statistically explain the variation in metabolism, and similarly, that the SNPs associated with changes in mRNA expression or metabolism will differ in different populations. These predictions suggest that because the loci involved in physiological responses are different from those affected by adaptive divergence, there is greater potential for adaptive change than if the same loci were involved. Additionally, we predict that there are many different ways to achieve a change in metabolism, which means that there are many different polymorphic loci that selection can act on. Providing the data to support or reject these hypotheses would enhance our understanding of evolutionary physiology and thus significantly influence our ability to predict climate change effects on species' abundance and survival.

第一部分：动物如何在不断变化的环境条件下生存和繁荣取决于它们的生理反应和基因的适应性变化。生理反应包括代谢率的变化和影响动物一生中代谢的蛋白质。这些可逆的、非遗传的变化在不断变化的环境中缓冲了个人的健康。然而，这些生理反应是有限的，动物可能会进化出影响其新陈代谢并提高其生存率的遗传变化。这项研究调查了生理和适应性变化之间的相互作用，以更好地了解影响健康和健身的机制。这项研究利用新的DNA测序技术来确定环境之间的10，000个遗传差异，并将这些差异与基因表达的测量相关联。这项研究的数据将告知社会在各种环境中增强健康的基因和基因表达。这项研究包括对来自不同经济和社会背景的本科生和高中生进行培训。第二部分：为了提高我们预测气候变化反应的能力，我们需要了解适应性反应的相对重要性和适应性差异的潜力。虽然有几个很好的脊椎动物模型之间的热适应分化类群和许多驯化反应的例子，很少有研究探讨驯化反应和适应分化之间的相互作用。为了研究这种相互作用，本研究首先测量代谢的变化和热适应的影响，然后使用这些措施的变化来询问mRNA和DNA变异的重要性。这项拟议研究的两个主要目标是利用测序进行基因分型，以提供数万个单核苷酸多态性，并应用RNA-Seq测量大多数表达的基因，以确定1）三个热流出物群体之间的核苷酸差异是否具有相似的适应性差异，以及2）mRNA表达的适应性和适应性变化是否影响相同的基因座。我们的预测是，mRNA表达的适应性和适应性变化涉及不同的基因，许多基因座可以影响代谢的变化，因此不同的人群将有不同的mRNA表达模式，统计学上解释代谢的变化，同样，与mRNA表达或代谢的变化相关的SNP在不同的人群中也会有所不同。这些预测表明，由于参与生理反应的位点与受适应性差异影响的位点不同，因此与参与相同位点的情况相比，适应性变化的潜力更大。此外，我们预测，有许多不同的方式来实现新陈代谢的变化，这意味着有许多不同的多态位点，选择可以采取行动。提供数据来支持或拒绝这些假设将提高我们的进化生理学的理解，从而显着影响我们的能力，预测气候变化对物种的丰度和生存的影响。

项目成果

期刊论文数量（13）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Effect of EBV-Transformation on Oxidative Phosphorylation Physiology in Human Cell lines

EBV 转化对人类细胞系氧化磷酸化生理学的影响

DOI：
10.1101/2019.12.16.878025
发表时间：
2020
期刊：
PloS one
影响因子：
3.7
作者：
Weinstein, Rachel N.;Crawford, Douglas
通讯作者：
Crawford, Douglas

Adaptation Without Boundaries: Population Genomics in Marine Systems

无国界适应：海洋系统中的群体基因组学

DOI：
10.1007/13836_2018_32
发表时间：
2020
期刊：
Population Genomics
影响因子：
0
作者：
Oleksiak, M. F.
通讯作者：
Oleksiak, M. F.

Feeling the heat: variation in thermal sensitivity within and among populations

感受热量：人群内部和人群之间热敏感性的差异

DOI：
10.1242/jeb.244831
发表时间：
2022
期刊：
Journal of Experimental Biology
影响因子：
2.8
作者：
DeLiberto, Amanda N.;Drown, Melissa K.;Ehrlich, Moritz A.;Oleksiak, Marjorie F.;Crawford, Douglas L.
通讯作者：
Crawford, Douglas L.

Marine Population Genomics: Challenges and Opportunities