The Behavioral Repertoire of Dinoflagellates: High-Speed, High-Resolution Imaging of Ecologically Important Species-Species Interactions

甲藻的行为全貌：对生态重要的物种间相互作用进行高速、高分辨率成像

• 批准号: 1559062
• 负责人: Houshuo Jiang
• 金额: $ 66.56万
• 依托单位: Woods Hole Oceanographic Institution
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-04-01 至 2021-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1559062&HistoricalAwards=false
• 关键词: Behavioral; Repertoire; Dinoflagellates; Speed; Resolution

Dinoflagellates are abundant and ecologically important species in marine ecosystems, and they play diverse roles in marine food webs. They may be best known to the general public as the organisms associated with periodic harmful algal blooms, or situations when toxins produced by fast-growing populations affect fish, shellfish, and marine mammals, as well as human use of coastal resources. These single-celled organisms have evolved a range of behaviors for obtaining nutrients from the environment, capturing prey, and evading predators. In this project, the investigators will use a newly developed high-speed microscale imaging system (HSMIS) in the lab and field to characterize dinoflagellate behaviors in unprecedented detail in space and time to understand ecologically important species-species interactions. The goal is to understand the relationships among behavior, morphology, and ecological function for dinoflagellates. More broadly, results will help explain processes that regulate the biomass of, distribution of, and chemical cycling by marine plankton. In addition, the investigators will develop and use a novel technology, provide research training for undergraduate students, and give demonstrations and lectures to the public.Dinoflagellates are one of the most abundant and ecologically important groups in marine ecosystems, playing diverse roles in marine food webs. These single-celled organisms have evolved an impressive repertoire of sensory, allelochemical, and behavioral capabilities for obtaining nutrients, capturing prey, evading predators, and competing against other organisms. This repertoire remains largely unexplored, however, in part because traditional microscopy limits observations at the relevant scales for the natural behavior of dinoflagellates. The investigators will conduct a laboratory and field project to use a high-speed microscale imaging system (HSMIS) to characterize dinoflagellate behavior in unprecedented spatial and temporal detail across a range of ecologically important species-species interactions. HSMIS overcomes several limitations inherent to traditional microscopy, such as wall effects due to small sample volumes, confinement to a horizontal field-of-view, and strong convection caused by using strong light. The goal is to achieve a mechanistic understanding of interspecies detection, avoidance, and capture. Observations will span a range of size scales and trophic levels and will include examples of different flagellar propulsive systems. Differences in swimming and flow will be used to understand trade-offs in body form and kinematics for nutrient uptake, resource competition, selective feeding, and predator/parasite avoidance.

甲藻是海洋生态系统中数量丰富、具有重要生态意义的物种，在海洋食物网中发挥着重要作用。公众最熟悉的是与周期性有害藻华有关的生物，或快速增长的种群产生的毒素影响鱼类、贝类和海洋哺乳动物以及人类对沿海资源的利用的情况。这些单细胞生物已经进化出一系列的行为来从环境中获取营养，捕获猎物和躲避捕食者。在该项目中，研究人员将在实验室和现场使用新开发的高速微尺度成像系统（HSMIS），在空间和时间上以前所未有的细节表征甲藻行为，以了解生态上重要的物种-物种相互作用。目的是了解甲藻的行为、形态和生态功能之间的关系。更广泛地说，研究结果将有助于解释调节海洋浮游生物生物量、分布和化学循环的过程。此外，研究人员还将开发和使用一种新技术，为本科生提供研究培训，并向公众进行演示和讲座。甲藻是海洋生态系统中数量最多、生态意义最重要的类群之一，在海洋食物网中发挥着多种作用。这些单细胞生物已经进化出一套令人印象深刻的感官、化感物质和行为能力，用于获取营养、捕获猎物、躲避捕食者和与其他生物竞争。然而，这一剧目在很大程度上仍未被探索，部分原因是传统的显微镜限制了对甲藻自然行为的相关尺度的观察。 研究人员将进行一个实验室和实地项目，使用高速微尺度成像系统（HSMIS）在一系列具有生态重要性的物种-物种相互作用中以前所未有的空间和时间细节来表征甲藻行为。HSMIS克服了传统显微镜固有的几个局限性，例如由于小样品体积引起的壁效应，限制到水平视场，以及使用强光引起的强对流。我们的目标是实现跨物种的检测，回避和捕获的机械理解。观察将跨越一系列的大小尺度和营养水平，并将包括不同的鞭毛推进系统的例子。在游泳和流动的差异将被用来了解权衡身体形态和运动学的营养吸收，资源竞争，选择性喂养，和捕食者/寄生虫避免。