I-Corps: Enzymes for on-site generation of dilute peracetic acid

I-Corps：现场生成稀过乙酸的酶

• 批准号: 1561788
• 负责人: Romas Kazlauskas
• 金额: $ 5万
• 依托单位: University of Minnesota-Twin Cities
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-10-15 至 2016-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1561788&HistoricalAwards=false
• 关键词: Corps; Enzymes; site; generation; dilute

Dilute peracetic acid (PAA) is a potent disinfectant for the food and beverage industry and for medical facilities and equipment. The estimated global market of peracetic acid in 2018 is US$600 million. The challenge with using PAA is that manufacture creates concentrated PAA solutions. These are hazardous to transport, to store and to dilute for use. The proposed solution is to make PAA on-site, which eliminates transport, make PAA only when needed, which eliminates storage, and make PAA only in the dilute less-hazardous form, which eliminates handling steps by workers. The key to this solution is an enzyme catalyzed reaction on-site from safer ingredients - acetic acid and hydrogen peroxide. To reach this application goal, the enzymes need to be more stable than they are now so they can be reused.Peracetic acid (PAA) is a strong, reactive oxidant which allows PAA to kill a broad range of microbes. This I-Corps team's goal is to produce PAA at end-user's on-site facility using enzymes by the perhydrolysis reaction of acetic acid and hydrogen peroxide. Prior research identified efficient enzymes which creates ten molecule of PAA per second for each molecule of enzyme. The fast enzyme catalyzed formation of PAA created the opportunity to make it at the application location only on demand and also in amounts needed. To make it commercially viable, the proposed approach is to use enzyme in a packed-bed reactor, with acetic acid and hydrogen peroxide fed in and produce PAA with the amounts needed. To achieve this demonstration, it requires engineering of perhydrolase enzymes to be more resistant for oxidation by PAA, immobilization of stabilized enzymes for the reusability and optimize reaction conditions for a continuous flow reactor. The current proposal seeks to commercialize the enzymatic production of PAA for safer use and thereby tests the broader hypothesis that the biotechnology micro factories can replace traditional chemical factories.

稀过氧乙酸（PAA）是食品和饮料行业以及医疗设施和设备的有效消毒剂。预计2018年过氧乙酸的全球市场为6亿美元。使用PAA的挑战在于制造商需要生产浓缩PAA溶液。这些物质在运输、储存和稀释使用时都是危险的。提出的解决方案是现场制造PAA，这消除了运输，仅在需要时制造PAA，这消除了储存，并且仅以稀释的较低危险形式制造PAA，这消除了工人的处理步骤。该解决方案的关键是由更安全的成分-乙酸和过氧化氢进行现场酶催化反应。为了达到这一应用目标，酶需要比现在更稳定，以便它们可以重复使用。过氧乙酸（PAA）是一种强活性氧化剂，可以杀死多种微生物。该I-Corps团队的目标是在最终用户的现场设施中使用酶通过醋酸和过氧化氢的过水解反应生产PAA。先前的研究发现了高效的酶，每一个酶分子每秒可以产生10个PAA分子。PAA的快速酶催化形成创造了仅根据需要并且还以所需的量在应用位置制备PAA的机会。为了使其具有商业可行性，所提出的方法是在填充床反应器中使用酶，加入乙酸和过氧化氢，并生产所需量的PAA。为了实现这一证明，它需要过水解酶的工程化以更耐PAA的氧化，固定化稳定的酶以实现可重复使用性，并优化连续流动反应器的反应条件。目前的提案旨在将PAA的酶促生产商业化，以更安全地使用，从而测试生物技术微型工厂可以取代传统化学工厂的更广泛的假设。