I-Corps: Enzymes for on-site generation of dilute peracetic acid

I-Corps：现场生成稀过乙酸的酶

• 批准号: 1561788
• 负责人: Romas Kazlauskas
• 金额: $ 5万
• 依托单位: University of Minnesota-Twin Cities
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-10-15 至 2016-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1561788&HistoricalAwards=false
• 关键词: Corps; Enzymes; site; generation; dilute

Dilute peracetic acid (PAA) is a potent disinfectant for the food and beverage industry and for medical facilities and equipment. The estimated global market of peracetic acid in 2018 is US$600 million. The challenge with using PAA is that manufacture creates concentrated PAA solutions. These are hazardous to transport, to store and to dilute for use. The proposed solution is to make PAA on-site, which eliminates transport, make PAA only when needed, which eliminates storage, and make PAA only in the dilute less-hazardous form, which eliminates handling steps by workers. The key to this solution is an enzyme catalyzed reaction on-site from safer ingredients - acetic acid and hydrogen peroxide. To reach this application goal, the enzymes need to be more stable than they are now so they can be reused.Peracetic acid (PAA) is a strong, reactive oxidant which allows PAA to kill a broad range of microbes. This I-Corps team's goal is to produce PAA at end-user's on-site facility using enzymes by the perhydrolysis reaction of acetic acid and hydrogen peroxide. Prior research identified efficient enzymes which creates ten molecule of PAA per second for each molecule of enzyme. The fast enzyme catalyzed formation of PAA created the opportunity to make it at the application location only on demand and also in amounts needed. To make it commercially viable, the proposed approach is to use enzyme in a packed-bed reactor, with acetic acid and hydrogen peroxide fed in and produce PAA with the amounts needed. To achieve this demonstration, it requires engineering of perhydrolase enzymes to be more resistant for oxidation by PAA, immobilization of stabilized enzymes for the reusability and optimize reaction conditions for a continuous flow reactor. The current proposal seeks to commercialize the enzymatic production of PAA for safer use and thereby tests the broader hypothesis that the biotechnology micro factories can replace traditional chemical factories.

稀乙酸（PAA）是食品和饮料行业以及医疗设施和设备的有效消毒剂。 2018年，估计全球过乙酸市场为6亿美元。使用PAA面临的挑战在于，制造会创建集中的PAA解决方案。这些是危险的运输，存储和稀释以供使用。提出的解决方案是在现场制造PAA，从而消除了运输，仅在需要时才能进行PAA，从而消除了存储，并仅以稀释的较少危险形式制作PAA，从而消除了工人的处理步骤。该溶液的关键是从更安全成分的酶催化反应 - 乙酸和过氧化氢。为了达到这个应用程序，酶需要比现在更稳定，以便可以重复使用。植酸（PAA）是一种强，反应性的氧化剂，使PAA可以杀死广泛的微生物。这个I-Corps团队的目标是使用乙酸和过氧化氢的核水解反应在最终用户的现场设施上生产PAA。先前的研究确定了有效的酶，该酶为每个酶分子创造了每秒10个PAA分子。快速酶催化PAA的形成创造了仅根据需求和所需数量才能在应用程序位置进行的机会。为了使其在商业上可行，提出的方法是在包装的反应堆中使用酶，乙酸和过氧化氢在进食中，并产生所需量的PAA。为了实现此次演示，它要求钙溶酶酶的工程对通过PAA的氧化更具耐药性，固定酶的固定酶可重复使用，并优化了连续流动反应器的反应条件。当前的提案旨在将PAA的酶促生产商业化，以便更安全使用，从而检验了更广泛的假设，即生物技术微型工厂可以取代传统的化学工厂。