DISSERTATION RESEARCH: Phylogeny and evolutionary exploration of the C3-CAM phenotypic space in Calandrinia (Montiaceae)

论文研究：Calandrinia（Montiaceae）C3-CAM 表型空间的系统发育和进化探索

基本信息

批准号：
1600971
负责人：
Erika Edwards
金额：
$ 2.08万
依托单位：
Brown University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2016
资助国家：
美国
起止时间：
2016-06-01 至 2017-05-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1600971&HistoricalAwards=false
关键词：
DISSERTATION RESEARCH Phylogeny evolutionary exploration

项目摘要

Crassulacean Acid Metabolism (CAM) photosynthesis is a complex set of traits that improves water use and photosynthetic efficiency in plants under drought and temperature stress. Calandrinia (Montiaceae) is an ideal lineage in which to investigate the evolutionary assembly of CAM photosynthesis. This research integrates phylogenetics, anatomy, ecology, physiology, greenhouse experiments, and gene expression studies to unravel the incremental assembly of CAM photosynthesis in Calandrinia. In light of ongoing global change and the increasing acidification of the global landscape (~40% of terrestrial Earth is already considered arid, semi-arid or sub-humid), CAM photosynthesis will likely play an important role in the future of carbon sequestration, sustainable biomass production, and food-crop security. With such imminent change ahead, it is more important than ever that we understand the evolutionary underpinnings of CAM photosynthesis, which may guide current efforts to engineer this syndrome into food and biofuel crops.The researchers will conduct a comparative gene expression study across species that represent C3, CAM, and CAM-intermediate types. They will use a comparative transcriptomic approach to answer the following questions: 1) how does differential gene expression of key CAM genes vary across the potentially intermediate types, 2) which gene copies are being recruited into new CAM function, 3) are there parallel nucleotide/ amino acid changes in gene copies recruited to perform CAM, and 4) how can transcriptome data help us to better differentiate and define these C3-CAM intermediates? Results from this study will yield the first lineage-based model of CAM evolution.

碎屑酸代谢（CAM）光合作用是一组复杂的特征，可提高干旱和温度压力下植物的用水和光合效率。 Calandrinia（Montiaceae）是研究CAM光合作用的进化组装的理想谱系。这项研究结合了系统发育，解剖学，生态学，生理学，温室实验和基因表达研究，以揭示Calandrinia中CAM光合作用的增量组装。鉴于全球持续的变化和全球景观的酸化（占地约40％已经被认为是干旱，半干旱或亚人类），CAM光合作用可能会在碳固执，可持续生物量生产和食品杂种安全的未来中起重要作用。随着即将发生的如此迫在眉睫的变化，我们比以往任何时候都重要，我们了解CAM光合作用的进化基础，这可以指导当前的努力将这种综合症设计为食品和生物燃料作物。研究人员将对代表C3，CAM和CAM互化类型的物种进行比较基因表达研究。 They will use a comparative transcriptomic approach to answer the following questions: 1) how does differential gene expression of key CAM genes vary across the potentially intermediate types, 2) which gene copies are being recruited into new CAM function, 3) are there parallel nucleotide/ amino acid changes in gene copies recruited to perform CAM, and 4) how can transcriptome data help us to better differentiate and define these C3-CAM intermediates?这项研究的结果将产生第一个基于谱系的CAM进化模型。