DISSERTATION RESEARCH: Gene Regulation Evolution and Speciation in House Mice

论文研究：家鼠的基因调控进化和物种形成

• 批准号: 1601699
• 负责人: Michael Nachman
• 金额: $ 2.04万
• 依托单位: University of California-Berkeley
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-07-01 至 2019-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1601699&HistoricalAwards=false
• 关键词: DISSERTATION; RESEARCH; Gene; Regulation; Evolution

This project will make use of controlled laboratory experiments utilizing the model system of house mice (Mus musculus) to understand the role of gene regulation in speciation. A primary goal of speciation research is to uncover the genetic differences between species that underlie reproductive isolation. While much of the work in this field has focused on identifying specific genes that contribute to reproductive isolation between species, gene regulation may play a large role in this process. Despite this, there are been few systematic attempts to link regulatory evolution to reproductive isolation. Characterizing regulatory evolution between house mouse subspecies will provide novel insight into how regulatory networks evolve and diverge which has broad implications for our understanding of the evolution of complex phenotypes as well as the speciation process. In addition, the investigators will engage in research with undergraduates from underrepresented groups and provide public and K12 outreach on this project.Taking advantage of a cross between house mouse subspecies, where hybrid dysfunction is largely unidirectional, the proposed work will use a variety of genomic methods (i.e., ChiP-sequencing, mRNA-sequencing, and ribosome profiling) to characterize changes in gene regulation on the transcriptional and translational level that are associated with hybrid sterility. This dissertation work will address the following questions: 1) What is the role of different levels of gene regulation to species divergence? and 2) What is the role of regulatory divergence in reproductive isolation between species? The work outlined here will be the first to: 1) characterize divergence in translational efficiency between mammalian species, and 2) examine the relationship between hybrid sterility and gene regulation on the transcriptional and translational level.

本项目将利用家鼠（小家鼠）模型系统的控制实验室实验来了解基因调控在物种形成中的作用。物种形成研究的一个主要目标是揭示导致生殖隔离的物种之间的遗传差异。虽然这一领域的大部分工作都集中在确定导致物种间生殖隔离的特定基因上，但基因调控可能在这一过程中发挥重要作用。尽管如此，很少有系统的尝试将调节进化与生殖隔离联系起来。表征家鼠亚种之间的调控进化将为调控网络如何进化和分化提供新的见解，这对我们理解复杂表型的进化以及物种形成过程具有广泛的意义。此外，研究人员将与来自代表性不足群体的本科生进行研究，并就该项目向公众和K12提供宣传。利用家鼠亚种之间的杂交，其中杂交功能障碍很大程度上是单向的，拟议的工作将使用各种基因组方法（即chip测序，mrna测序和核糖体分析）来表征与杂交不育相关的转录和翻译水平上基因调控的变化。本论文将解决以下问题：1)不同水平的基因调控对物种分化的作用是什么？2)调控差异在物种间生殖隔离中的作用是什么？本文概述的工作将是第一个：1)表征哺乳动物物种之间翻译效率的差异；2)在转录和翻译水平上研究杂交不育与基因调控之间的关系。