INTRACELLULAR ELECTROCHEMISTRY WITH CARBON NANOTUBE-BASED SENSORS

基于碳纳米管的传感器的细胞内电化学

• 批准号: 1604893
• 负责人: Michael Schrlau
• 金额: $ 29.41万
• 依托单位: Rochester Institute of Tech
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-01 至 2020-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1604893&HistoricalAwards=false
• 关键词: INTRACELLULAR; ELECTROCHEMISTRY; CARBON; NANOTUBE; BASED

PI: Schrlau, Michael Proposal Number: 1604893This project proposes to create a very tiny biosensor for identifying and quantifying biomolecules inside a single living cell. The investigator proposes a novel way of performing selective measurement inside living cells, (instead of currently used methods of breaking open the cell), and after proving the approach plan is to quantify cellular metabolic state in real time.The goal of the project is to create the ability to identify and quantify biomolecules inside a single living cell with minimal perturbation and over long periods of time using electrochemical techniques. Multifunctional nanoprobe will be constructed, capable of injecting fluids in order to conduct self-contained electrochemical measurements inside confined aqueous microenvironments. The nanoprobe is then utilized to inject a reaction-enabling substrate into the cell for selectively quantifying senescence-associated beta-galactosidase, a cytosolic enzyme, an indicator of many chronic diseases. It is proposed to use carbon nanotube (CNT) -based nanoprobes will provide the ability for selectively quantifying cell senescence in real-time. It is anticipated that at the conclusion of the project, there will be a new analytical tool and technique for quantifying electroactive biomolecules within single living cells. Simple, scalable template-based nanomanufacturing processes will be employed to integrate the nanoelectrodes into the tip of a pulled glass capillary without nanoassembly. The CNT-based nanoprobe is designed to readily fit standard cell physiology instruments, thus facilitating easy technological dissemination, broad utilization, and potential commercialization. The outcome of this proposal is likely to provide a first-in-class CNT-based tool for a novel minimally invasive analytical technique for gathering quantitative data from single living cells. The proposed efforts will generate new insights into fundamental cell physiology, which will ultimately help identify the early onset of diseases, facilitate drug development and improve therapeutic efficacy. More broadly, the development of intracellular electrochemistry will advance scientific knowledge in nanotechnology, materials science and engineering, electrochemistry, cell biology, and biomedicine. Further, the project will provide several high-impact research and educational opportunities for students at all levels as well as recruits and will support underrepresented groups in STEM.

PI: Schrlau， Michael提案号：1604893该项目提出创建一个非常微小的生物传感器，用于识别和定量单个活细胞内的生物分子。研究者提出了一种在活细胞内进行选择性测量的新方法，（而不是目前使用的破开细胞的方法），并证明了该方法计划是实时量化细胞代谢状态。该项目的目标是利用电化学技术，在最小扰动和长时间内，创造识别和量化单个活细胞内生物分子的能力。多功能纳米探针将被构建，能够注入流体，以便在受限的水微环境中进行独立的电化学测量。然后利用纳米探针向细胞中注射一种使反应发生的底物，选择性地定量衰老相关的β -半乳糖苷酶，这是一种胞质酶，是许多慢性疾病的指标。提出利用碳纳米管（CNT）为基础的纳米探针将提供选择性定量细胞衰老的实时能力。预计在项目结束时，将有一种新的分析工具和技术来定量单个活细胞内的电活性生物分子。简单的，可扩展的基于模板的纳米制造工艺将被用于将纳米电极集成到拉玻璃毛细管的尖端，而无需纳米组装。基于碳纳米管的纳米探针可以很容易地适应标准的细胞生理学仪器，从而促进了技术的传播、广泛的应用和潜在的商业化。该提案的结果可能提供一流的基于碳纳米管的工具，用于从单个活细胞收集定量数据的新型微创分析技术。拟议的努力将产生对基本细胞生理学的新见解，这将最终有助于确定疾病的早期发病，促进药物开发和提高治疗效果。更广泛地说，细胞内电化学的发展将推动纳米技术、材料科学与工程、电化学、细胞生物学和生物医学的科学知识。此外，该项目将为各级学生和新兵提供一些高影响力的研究和教育机会，并将支持STEM中代表性不足的群体。