Preclinical assessment of decellularised (antigen-reduced), gene-modified porcine heart valves (C07)

脱细胞（抗原减少）基因修饰猪心脏瓣膜的临床前评估 (C07)

• 批准号: 223965276
• 金额: --
• 依托单位: Klinik für Herz-, Thorax-, Transplantations- und Gefäßchirurgie (HTTG)
• 依托单位国家: 德国
• 项目类别: CRC/Transregios
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/223965276?language=en
• 关键词: Preclinical; assessment; decellularised; antigen; reduced

The goal of this research proposal is to use the domestic pig as a source for regenerative heart valve implants. Prior to clinical trials, we will continue with the ongoing evaluations of pulmonary heart valves derived from GGTA1-ko pigs that had been decellularised and partially deglycosylated and finally implanted in our orthotopic baboon model. In the new funding period, we will develop and validate more effective deglycosylation regimes for decellularised heart valve matrices derived from multi-ko pigs. These will be characterised using glycan profiling. Preformed antibodies will be evaluated in vitro, using blood and serum from potential patients. The respective optimised valves will then finally be tested in vivo using our baboon implantation model.

本研究提案的目标是使用家猪作为再生心脏瓣膜植入物的来源。在临床试验之前，我们将继续对来自GGTA 1-ko猪的肺动脉心脏瓣膜进行持续评价，这些猪已经去细胞化和部分去糖基化，并最终植入我们的原位狒狒模型中。在新的资助期内，我们将开发和验证更有效的去糖基化方案，用于来自多ko猪的去细胞心脏瓣膜基质。这些将使用聚糖谱进行表征。将使用来自潜在患者的血液和血清在体外评价预制抗体。最后将使用我们的狒狒植入模型对相应的优化瓣膜进行体内测试。