Demonstration and Applications of Single Molecule Magnetic Resonance Spectroscopy at Physiological Conditions

生理条件下单分子磁共振波谱的演示和应用

• 批准号: 1611134
• 负责人: Susumu Takahashi
• 金额: $ 39万
• 依托单位: University of Southern California
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-01 至 2020-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1611134&HistoricalAwards=false
• 关键词: Demonstration; Applications; Single; Molecule; Magnetic

With support from the Chemical Measurement & Imaging (CMI) and the Chemical Structure, Dynamics, & Mechanisms - A (CSDM-A) Programs in the Division of Chemistry, Dr. Susumu Takahashi and his group at the University of Southern California are devising means of improving magnetic resonance (MR) techniques in order to enable application of these powerful characterization tools to study the structure and dynamics of biological molecules down to the level of single molecules. Dr. Takahashi is also developing educational/training programs and materials that will help explain these methods and the chemistry they help to explain to USC graduate and undergraduate students, as well as to high school and elementary school students in USC's neighborhood community.Magnetic resonance spectrometry techniques, such as nuclear magnetic resonance (NMR) and electron paramagnetic resonance (EPR), can provide detailed information about molecular structure and dynamics, but are hampered by intrinsically low sensitivity. Recent technological advances, particularly those utilizing a single nitrogen-vacancy (NV) center in diamond as a sensor, have enabled detection of a single electron spin. Dr. Takahashi seeks to demonstrate MR analysis of a few molecules under physiological conditions using NV centers and high-frequency/high-field EPR. Success in the research work will provide a promising pathway to enable EPR investigation of single biological molecules containing either native electron spin centers or extrinsic spin labels.

在化学系化学测量成像（CMI）和化学结构、动力学、机制- A（CSDM-A）项目的支持下，南加州大学的Susumu Takahashi博士及其团队正在设计改进磁共振（MR）技术的方法，以便能够应用这些强大的表征工具来研究生物分子的结构和动力学，直至单分子水平。 高桥博士还在开发教育/培训项目和材料，这些项目和材料将有助于向南加州大学的研究生和本科生，以及南加州大学附近社区的高中生和小学生解释这些方法和他们帮助解释的化学磁共振光谱技术，如核磁共振（NMR）和电子顺磁共振（EPR）可以提供有关分子结构和动力学的详细信息，但受到固有的低灵敏度的阻碍。 最近的技术进步，特别是那些利用金刚石中的单个氮空位（NV）中心作为传感器的技术进步，已经能够检测单个电子自旋。 Takahashi博士试图证明在生理条件下使用NV中心和高频/高场EPR对一些分子进行MR分析。 研究工作的成功将为EPR研究含有天然电子自旋中心或外源自旋标记的单个生物分子提供一条有前途的途径。

项目成果

DNP-NMR of surface hydrogen on silicon microparticles

硅微粒表面氢的 DNP-NMR

• DOI: 10.1016/j.ssnmr.2019.04.008
• 发表时间: 2019
• 期刊: Solid State Nuclear Magnetic Resonance
• 影响因子: 3.2
• 作者: Shimon, Daphna;van Schooten, Kipp J.;Paul, Subhradip;Peng, Zaili;Takahashi, Susumu;Köckenberger, Walter;Ramanathan, Chandrasekhar
• 通讯作者: Ramanathan, Chandrasekhar

Reduction of surface spin-induced electron spin relaxations in nanodiamonds

• DOI: 10.1063/5.0007599
• 发表时间: 2020-08
• 期刊: arXiv: Mesoscale and Nanoscale Physics
• 作者: Zaili Peng;Jax Dallas;Susumu Takahashi

Investigation of near-surface defects of nanodiamonds by high-frequency EPR and DFT calculation

• DOI: 10.1063/1.5085351
• 发表时间: 2019-04-07
• 期刊: JOURNAL OF CHEMICAL PHYSICS
• 影响因子: 4.4
• 作者: Peng, Z.;Biktagirov, T.;Takahashi, S.
• 通讯作者: Takahashi, S.

Understanding the Linewidth of the ESR Spectrum Detected by a Single NV Center in Diamond

• DOI: 10.1021/acs.jpca.9b02445
• 发表时间: 2019-07-25
• 期刊: JOURNAL OF PHYSICAL CHEMISTRY A
• 影响因子: 2.9
• 作者: Fortman, Benjamin;Takahashi, Susumu
• 通讯作者: Takahashi, Susumu

Investigation of Coherence Time of a Nitrogen-Vacancy Center in Diamond Created by a Low-Energy Nitrogen Implantation

• DOI: 10.1007/s00723-017-0885-x
• 发表时间: 2017-06-01
• 期刊: APPLIED MAGNETIC RESONANCE
• 影响因子: 1
• 作者: Abeywardana, Chathuranga;Peng, Zaili;Takahashi, Susumu
• 通讯作者: Takahashi, Susumu