Removal of time and fidelity constraints in MALDI-MS imaging of proteins in tissue

消除组织中蛋白质 MALDI-MS 成像的时间和保真度限制

• 批准号: 1611538
• 负责人: Franco Basile
• 金额: $ 35万
• 依托单位: University of Wyoming
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-01 至 2021-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1611538&HistoricalAwards=false
• 关键词: Removal; time; fidelity; constraints; MALDI

With support from the Chemical Measurement and Imaging Program in the Division of Chemistry, Prof. Franco Basile and his group at the University of Wyoming are working to develop new means of determining the composition and distribution of proteins in biological samples, employing the powerful analytical tools of mass spectrometry (MS). Specifically, the Basile group is developing new means of using MS to form an image of the location of specific (bio)chemicals on biological samples, including animal or plant tissue. These tools can be used widely in investigations of fundamental biology, biomedical research, renewable energy (biofuels) and bio-inspired materials, all areas that benefit society. Students involved receive broad, interdisciplinary training, preparing them for careers in a range of disciplines in industry and academia. The project is also fostering collaborative research within the University of Wyoming, and involving faculty and students from neighboring institutions (including community colleges) and others in the broader MS-imaging scientific community.The Basile team is developing a rapid (seconds) and solvent-free method to thermally digest and decompose proteins in biological samples. This represents a significant improvement over current methodologies, which require time-consuming (4h to overnight) digestion of proteins with expensive and difficult-to-use enzymes in solution. The non-enzymatic and solvent-free approach for on-tissue protein digestion is not only ~500 times faster than traditional enzymatic digestion, but also provides more control over the area of the tissue that is analyzed. The new method utilizes well-characterized thermal protein decomposition reactions that were discovered and developed in the Basile laboratory over the last 10 years. Potential applications span a wide range of disciplines, broadening the training received by participating students.

在化学系化学测量和成像项目的支持下，怀俄明大学 Franco Basile 教授和他的团队正在致力于开发新方法，利用质谱 (MS) 等强大的分析工具来确定生物样品中蛋白质的组成和分布。 具体来说，Basile 小组正在开发使用 MS 来形成生物样本（包括动物或植物组织）上特定（生物）化学物质位置的图像的新方法。 这些工具可广泛用于基础生物学、生物医学研究、可再生能源（生物燃料）和仿生材料的研究，以及所有造福社会的领域。 参与的学生接受广泛的跨学科培训，为他们在工业界和学术界的一系列学科的职业生涯做好准备。 该项目还促进怀俄明大学内部的合作研究，并让邻近机构（包括社区学院）的教职员工和学生以及更广泛的 MS 成像科学界的其他人员参与进来。Basile 团队正在开发一种快速（秒）且无溶剂的方法来热消化和分解生物样品中的蛋白质。 这代表了对当前方法的重大改进，当前方法需要使用昂贵且难以使用的溶液中的酶来耗时（4 小时到过夜）消化蛋白质。 用于组织蛋白质消化的非酶和无溶剂方法不仅比传统酶消化快约 500 倍，而且还可以更好地控制分析的组织区域。 新方法利用了 Basile 实验室在过去 10 年中发现和开发的充分表征的热蛋白质分解反应。 潜在的应用涵盖广泛的学科，扩大了参与学生接受的培训。