MRI: Acquisition of a MALDI-ToF/ToF-MS Instrument for Chemical and Biological Research

MRI：购买用于化学和生物研究的 MALDI-ToF/ToF-MS 仪器

• 批准号: 1429615
• 负责人: Franco Basile
• 金额: $ 35万
• 依托单位: University of Wyoming
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-08-01 至 2017-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1429615&HistoricalAwards=false
• 关键词: MRI; Acquisition; MALDI; ToF; MS

With this award from the Major Research Instrumentation program (MRI) and the Chemistry Research Instrumentation program (CRIF), the University of Wyoming will acquire a time of flight mass spectrometer with matrix assisted laser desorption ionization technology and a liquid chromatograph (MALDI-TOF-LCMS). In general, mass spectrometry (MS) is one of the key analytical methods used to identify and characterize small quantities of chemical species embedded in complex matrices. A laser impinging on the inert matrix embedded with the sample, vaporizes and ionizes the sample. The ions pass into the mass spectrometer where the masses of the parent ion and its fragment ions are measured. This highly sensitive technique allows identification and determination of the structure of molecules in a complex mixture. An instrument with a liquid chromatograph provides additional identification power by separating mixtures of compounds before they are mass analyzed. The acquisition will strengthen the research infrastructure at the University and regional area. High school, undergraduate and graduate students from rural areas, including Native Americans, Hispanic/Latinos, women, and first-generation college students in Wyoming and surrounding Frontier states will benefit from training with this modern analytical technique. The proposal is aimed at enhancing research and education at all levels, especially in areas such as (a) developing analytical methodologies for the rapid and precise analysis of biological and synthetic samples; (b) deciphering of protein folding dynamics and anti-freeze protein function; (c) developing photocatalytic organic materials; (d) developing glycosylated proteins; (e) elucidating cell messaging pathways; (f) studying fundamentals of evolutionary cell biology; and (g) developing clean energy sources.

凭借主要研究仪器计划（MRI）和化学研究仪器计划（CRIF）的这一奖项，怀俄明州大学将获得具有基质辅助激光解吸电离技术的飞行时间质谱仪和液相色谱仪（MALDI-TOF-LCMS）。 一般来说，质谱（MS）是用于识别和表征嵌入复杂基质中的少量化学物质的关键分析方法之一。 激光照射在嵌入有样品的惰性基质上，使样品蒸发和电离。 离子进入质谱仪，在那里测量母离子及其碎片离子的质量。这种高灵敏度的技术可以识别和确定复杂混合物中分子的结构。带有液相色谱仪的仪器通过在进行质量分析之前分离化合物的混合物来提供额外的鉴别能力。 此次收购将加强大学和区域的研究基础设施。来自农村地区的高中生、本科生和研究生，包括美国原住民、西班牙裔/拉丁裔、女性以及怀俄明州和周边边境州的第一代大学生，将受益于这种现代分析技术的培训。该提案旨在加强各级的研究和教育，特别是在以下领域：（a）开发快速和精确分析生物和合成样品的分析方法;（B）破译蛋白质折叠动力学和防冻蛋白质功能;（c）开发光催化有机材料;（d）开发糖基化蛋白质;（e）阐明细胞信息传递途径;（f）研究进化细胞生物学的基本原理;及（g）发展清洁能源。