CAREER: Rapid Non-Enzymatic Sample Preparation for Mass Spectrometry

职业：用于质谱分析的快速非酶样品制备

• 批准号: 0844694
• 负责人: Franco Basile
• 金额: $ 40万
• 依托单位: University of Wyoming
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0844694&HistoricalAwards=false
• 关键词: CAREER; Rapid; Non; Enzymatic; Sample

Professor Franco Basile at the University of Wyoming is supported by the Analytical and Surface Chemistry Program in the Chemistry Division to study a challenging area of an important analytical detection method - mass spectrometry. Specifically, Professor Basile's research centers on the use of non-enzymatic processes (e.g., microwave digestion, pyrolysis, and electrochemistry) as alternatives to typical enzymatic digestion for pre-treatment of biological samples for mass spectrometric analysis. The research combines historical techniques with modern practices. Outcomes of Dr. Basile's work are likely to make a significant contribution to many areas of mass spectrometry. For example, field portable mass spectrometers would greatly benefit from shortened sample preparation protocols and the dramatically reduced need for reagents - focus areas for this research project.Dr. Basile integrates aspects of this work with the Summer Research in Analytical Chemistry (SRAC) Program, which includes participation of high school students and their teachers from around the State of Wyoming. The program fosters independent research skills and excitement about science for students, and equips teachers with better understanding of some of the tools used in modern analytical chemistry techniques.

怀俄明州大学的Franco Basile教授得到化学系分析和表面化学项目的支持，研究一种重要的分析检测方法-质谱法的一个具有挑战性的领域。具体来说，Basile教授的研究中心是使用非酶过程（例如，微波消化、热解和电化学）作为典型酶消化的替代物，用于质谱分析的生物样品的预处理。该研究将历史技术与现代实践相结合。Basile博士的工作成果可能会对质谱分析的许多领域做出重大贡献。例如，现场便携式质谱仪将大大受益于缩短的样品制备方案和大大减少对试剂的需求-本研究项目的重点领域。Basile博士将这项工作的各个方面与夏季分析化学研究（SRAC）计划相结合，该计划包括来自怀俄明州各地的高中学生和教师的参与。该计划培养学生的独立研究技能和对科学的兴奋，并使教师更好地了解现代分析化学技术中使用的一些工具。