NSF NPGI Postdoctoral Fellowship in Biology FY 2016

2016 财年 NSF NPGI 生物学博士后奖学金

• 批准号: 1611875
• 负责人: Kevin Potter
• 金额: $ 21.6万
• 依托单位: Potter Kevin
• 依托单位国家: 美国
• 项目类别: Fellowship Award
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-06-01 至 2019-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1611875&HistoricalAwards=false
• 关键词: NSF; NPGI; Postdoctoral; Fellowship; Biology

This action funds an NSF National Plant Genome Initiative Postdoctoral Research Fellowship in Biology for FY 2015. The fellowship supports a research and training plan in a host laboratory for the Fellow who also presents a plan to broaden participation in biology. The title of the research and training plan for this fellowship to Kevin Potter is "Uncovering the transcriptional and genomic dynamics of cytokinin signaling in rice". The host institution for the fellowship is the University of North Carolina in Chapel Hill and the sponsoring scientist is Dr. Joseph Kieber.Cytokinins are plant hormones that are known to regulate various aspects of growth and development and other physiological processes in plants. The overarching goal of the project is to gain a deeper understanding into how cytokinin regulates these processes by using an integrative transcriptomic and genomic approach to build a dynamic model of the cytokinin response in rice. These new insights may provide new effective strategies to engineer this signaling pathway to modify various agronomical traits in rice needed to meet the future food supply and demand. The broader impacts of the project consist of training undergraduate and graduate students, assisting the Morehead Planetarium and Science Center with a series of workshops designed for UNC graduate students, and participating in the North Carolina DNA Day, which is an annual event where North Carolina researchers from various institutions advocate careers in science and biotechnology and give biology lessons to high schools throughout North Carolina. Training objectives include acquiring expertise with new transcriptomic and genomics methods, and bioinformatics tools.This project seeks to: 1) kinetically characterize the transcriptional and open chromatin profiles using RNA-Seq and formaldehyde-assisted isolation of regulatory elements (FAIRE-seq), respectively, in response to cytokinins in shoots and roots in rice; 2) integrate these datasets, build dynamic models for the cytokinin response, and identify cis-acting regulatory elements by utilizing a variety of bioinformatics tools; and 3) adapt assay for transposase accessible chromatin followed by sequencing (ATAC-seq) for use in rice and then use this method in conjunction with RNA-Seq to build dynamic tissue-specific cytokinin response models in the shoot and root meristems in rice. These models will be tested and validated in future studies by altering different modules and components to ascertain their specific function in the cytokinin signaling pathway and effects on agronomical traits in rice. The collected sequencing datasets will be made available at the Sequence Read Archive at NCBI.

该行动资助了2015财年NSF国家植物基因组计划生物学博士后研究奖学金。该研究金支持研究员在东道实验室的研究和培训计划，研究员还提出了扩大生物学参与的计划。凯文·波特的研究和培训计划的标题是“揭示水稻细胞分裂素信号的转录和基因组动力学”。该研究金的主办机构是位于查佩尔山的北卡罗来纳州大学，赞助科学家是约瑟夫·基伯博士。细胞分裂素是一种植物激素，众所周知，它调节植物生长和发育的各个方面以及其他生理过程。该项目的总体目标是通过使用整合的转录组学和基因组学方法来建立水稻细胞分裂素反应的动态模型，从而更深入地了解细胞分裂素如何调节这些过程。这些新的见解可能会提供新的有效策略来工程化这一信号通路，以改变水稻的各种农艺性状，以满足未来的粮食供应和需求。该项目更广泛的影响包括培训本科生和研究生，协助莫尔黑德天文馆和科学中心为研究生举办一系列讲习班，并参加北卡罗来纳州DNA日活动，这是一项年度活动，北卡罗来纳州各机构的研究人员在该活动中倡导科学和生物技术职业，并为整个北卡罗来纳州的高中开设生物课。本项目旨在：1）利用RNA-Seq和甲醛辅助分离调控元件，对转录和开放染色质谱进行动力学表征（FAIRE-seq）分别响应于水稻芽和根中的细胞分裂素; 2）整合这些数据集，建立细胞分裂素反应的动态模型，并利用各种生物信息学工具鉴定顺式作用调控元件;和3）调整转座酶可及染色质的测定，然后测序（ATAC-seq）用于水稻，然后使用这种方法与RNA-Seq结合来构建动态组织-水稻茎和根分生组织中特异性细胞分裂素反应模型。这些模型将在未来的研究中进行测试和验证，通过改变不同的模块和组件，以确定其在细胞分裂素信号通路中的特定功能和对水稻农艺性状的影响。收集的测序数据集将在NCBI的Sequence Read Archive中提供。