Formation of ellagic acid precursors in Fragaria

草莓中鞣花酸前体的形成

• 批准号: 224621359
• 负责人: Professor Dr. Wilfried Schwab
• 金额: --
• 依托单位: Professur für Biotechnologie der Naturstoffe (BiNa)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2012
• 资助国家: 德国
• 起止时间: 2011-12-31 至 2015-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/224621359?language=en
• 关键词: Formation; ellagic; acid; precursors; Fragaria

Strawberry (Fragaria x ananassa) contains high amounts of bioactive polyphenols, in particular ellagic acid/ellagitannins. Recent significant findings strongly suggest that ellagic acid/ellagitannins provide effective protection against diverse human diseases including cardiovascular diseases, neurodegenerative diseases, and hormonal cancers like prostate and breast cancer. Ellagitannins possess strong antimicrobial activity towards bacteria, yeasts and viruses and thus serve as natural defence against diverse range of microbial infections. Although the biological significance of these compounds is widely acknowledged the genetic background of their biosynthetic pathway is almost unknown.We are planning to clone and heterologously express genes coding for putative gallate:UDP-glucose glucosyltransferases (GT) and gallate acyltransferases (AT) assumed to be involved in the formation of ellagitannin precursors. Candidate genes will be identified on the basis of sequence information of biochemically characterized (hydroxyl)benzoate GTs and acyl-glucose dependent ATs in combination with expression analysis performed by next-generation sequencing (NGS) and quantitative PCR. Alternatively, we will isolate and purify ellagitannin-pathway enzymes from F. x ananassa by activity guided assays. The proteins will be sequenced and the corresponding open reading frames extracted from the recently published genome sequence of F. vesca. It is supposed that both Fragaria species share a common ancestor. Recombinant proteins will be expressed in Escherichia coli or Saccharomyces cerevisiae and functionally characterized. The data can be used to develop molecular markers for high ellagic acid/ellagitannin content that will shorten the breeding period towards cultivars with additional health benefits. Besides, the result can be applied for the biotechnological production of ellagic acid derivatives.

草莓（Fragaria x ananassa）含有大量的生物活性多酚，特别是鞣花酸/鞣花单宁。最近的重大发现强烈表明，鞣花酸/鞣花单宁提供有效的保护，以防止各种人类疾病，包括心血管疾病，神经退行性疾病，和激素癌症，如前列腺癌和乳腺癌。鞣花单宁对细菌、酵母和病毒具有很强的抗微生物活性，因此可以作为抵御各种微生物感染的天然防御。虽然这些化合物的生物学意义是公认的，其生物合成途径的遗传背景几乎是unknow.We计划克隆和异源表达基因编码推定的没食子酸：UDP-葡萄糖葡萄糖基转移酶（GT）和没食子酸酰基转移酶（AT）假定参与形成鞣花单宁前体。候选基因将根据生物化学表征的（羟基）苯甲酸GT和酰基葡萄糖依赖性AT的序列信息，结合通过下一代测序（NGS）和定量PCR进行的表达分析进行鉴定。或者，我们将从F. X ananassa通过活性引导测定。这些蛋白质将被测序，并从最近公布的F.维斯卡据推测，这两种草莓有着共同的祖先。重组蛋白将在大肠杆菌或酿酒酵母中表达并进行功能表征。这些数据可用于开发高鞣花酸/鞣花单宁含量的分子标记，这将缩短育种周期，使品种具有额外的健康益处。此外，该研究结果可应用于鞣花酸衍生物的生物技术生产。

项目成果

Functional Characterization and Substrate Promiscuity of UGT71 Glycosyltransferases from Strawberry (Fragaria × ananassa).

• DOI: 10.1093/pcp/pcv151
• 发表时间: 2015-12
• 期刊: Plant & cell physiology
• 影响因子: 4.9
• 作者: Chuankui Song;Le Gu;Jingyi Liu;Shuai Zhao;X. Hong;Katja Schulenburg;W. Schwab