SBIR Phase I: Portable System for Detection of Harmful Algal Bloom Toxins in Freshwater and Marine Environments

SBIR 第一阶段：用于检测淡水和海洋环境中有害藻华毒素的便携式系统

• 批准号: 1621951
• 负责人: Michael Lochhead
• 金额: $ 21.77万
• 依托单位: MBio Diagnostics, Inc.
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-07-01 至 2017-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1621951&HistoricalAwards=false
• 关键词: SBIR; Phase; Portable; System; Detection

The broader impact/commercial potential of this Small Business Innovation Research Phase I project will be to initiate development of an effective technology that will enable users in the field to perform laboratory-quality cyanotoxin testing to help protect drinking water, monitor commercial food resources, and provide critical data for ecosystems management. Harmful algal blooms (HABs) in United States and global freshwater and marine environments are increasing in frequency and duration and constitute a growing public health threat while also carrying substantial economic, ecologic, and food supply implications. The goal is to initially launch a system that delivers a panel of water toxin results in less than 15 minutes. Validation will be with customers in public health and regulatory agencies followed by placements with water resource managers of commercial drinking water systems. The technical objectives in this Phase I research project are designed to provide a user-friendly platform with a unique combination of attributes. A cost competitive system that delivers multiple immunoassay results from a single sample using a single, simple protocol, provides lab quality accuracy and reliability, and connected data management incorporating smart phone infrastructure and cloud-based systems. This Phase I project is designed to deliver three key technical objectives. (1) One-Step Assay - deliver a single-step, 15-minute duplex microcystin / cylindrospermopsin assay tuned to drinking water recommendations. (2) Sample Prep - establish preliminary component designs for the water sample-to-cartridge interface, including designs that enable intra-cellular and dissolved toxin methods. (3) Connectivity - develop the electronics and software infrastructure plan for distributed systems networking.

这个小企业创新研究第一阶段项目的更广泛的影响/商业潜力将是启动一项有效技术的开发，使该领域的用户能够进行实验室质量的氰毒素测试，以帮助保护饮用水，监测商业食品资源，并为生态系统管理提供关键数据。有害藻华（HABs）在美国和全球淡水和海洋环境中的频率和持续时间都在增加，并构成日益严重的公共卫生威胁，同时也带来了重大的经济，生态和食品供应的影响。我们的目标是最初推出一个系统，在不到15分钟的时间内提供一组水毒素结果。验证将与公共卫生和监管机构的客户一起进行，然后与商业饮用水系统的水资源管理人员一起工作。第一阶段研究项目的技术目标旨在提供一个具有独特属性组合的用户友好平台。一个具有成本竞争力的系统，使用单一、简单的协议从单个样本中提供多个免疫分析结果，提供实验室质量准确性和可靠性，并结合智能手机基础设施和基于云的系统进行连接数据管理。第一阶段项目旨在实现三个关键技术目标。(1)一步检测-提供一个单一的步骤，15分钟的双重微囊藻毒素/柱孢菌素检测调整到饮用水的建议。(2)样品制备-为水样-检测盒接口建立初步组件设计，包括能够实现细胞内和溶解毒素方法的设计。(3)连通性-为分布式系统网络开发电子和软件基础设施计划。