MRI: Acquisition of a Nikon SIM & STORM capable super-resolution fluorescent microscope as a shared instrument for the Penn State research community

MRI：购买 Nikon SIM

• 批准号: 1625473
• 负责人: Charles Anderson
• 金额: $ 64.93万
• 依托单位: Pennsylvania State Univ University Park
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-01 至 2019-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1625473&HistoricalAwards=false
• 关键词: MRI; Acquisition; Nikon; SIM; &amp

An award is made to The Pennsylvania State University, University Park campus to purchase a super-resolution microscope that will enable the capture of images of plant and animal cells, as well as complex chemical samples, at the scale of single molecules. This microscope will reveal new insights into how living and chemical systems are organized and work. The project will also generate new image analysis tools for the scientific community. The microscope will enable interdisciplinary research training and enhance education through coursework and outreach to other Penn State campuses and other institutions. Integration of this microscope into a core microscopy facility will make it available to undergraduate, graduate and postdoctoral trainees, and regular imaging workshops will be offered by Penn State. New teaching modules for K-12 and undergraduate educators demonstrating the science of size and the potential of super-resolution microscopy will be developed. Access and training will be assured for underrepresented students through programs including the Summer Experience in the Eberly College of Science, McNair Scholars, Women in Science and Engineering Research, and Minority Undergraduate Research Experience. Public understanding of super-resolution microscopy and its advantages will be catalyzed by multiple outreach activities and venues, including The Franklin Institute (science museum) and Penn State's Ag Progress Days, which together will expose this cutting-edge imaging technology to tens of thousands of people. The discoveries enabled by this microscope will advance the study of plant and animal development, sustainable agriculture and energy production, and the chemical interactions that define our physical environment.Super-resolution microscopy is leading to dramatic new research opportunities in the life sciences, and has emerging capabilities in chemistry and materials research. Penn State will advance our optical imaging-based research through the purchase of a super-resolution microscope with both Structured Illumination Microscopy (SIM) and Stochastic Optical Reconstruction Microscopy (STORM) capabilities. Both imaging modules are combined on a single microscope platform, and use high-magnification total internal reflection fluorescence (TIRF) objectives, an automated focus retention system, and an integrated multi-channel solid state laser launch. The ease of use of this commercial super-resolution microscope is a particularly appealing feature for equipment residing within Penn State's Huck Institutes of the Life Sciences Microscopy & Cytometry Facility, a multi-user core facility. Research and training with this microscope will focus on four main areas: (a) plant cell biology; (b) animal cell biology; (c) chemistry; and (d) advanced image processing and pattern recognition. Researchers in groups (a)-(c) will leverage the increased resolution offered by this system to push their research into hitherto unreachable areas of fine-scale biological processes and molecular interactions. In parallel, group (d) will collaborate with those generating super-resolution imaging data to advance our ability to recognize structures and patterns from molecular localization data. The combined SIM/STORM microscope will maximally benefit the largest number of researchers in the life and chemical sciences at Penn State by offering a high degree of flexibility for sample labeling and imaging. As an integral component of this project, faculty with research interests in advanced image analysis and pattern recognition will generate new algorithms for analyzing SIM and STORM imaging data that will be widely applicable to super-resolution microscopy and will be freely disseminated to the global scientific community.

向宾夕法尼亚州立大学（University Park Campus）颁发了奖励，以购买超分辨率显微镜，该显微镜将在单分子的规模上捕获动植物的图像以及复杂的化学样品。该显微镜将揭示有关生活和化学系统如何组织和工作的新见解。该项目还将为科学界生成新的图像分析工具。显微镜将通过课程工作和向其他宾夕法尼亚州立大学校园和其他机构进行宣传来实现跨学科研究培训并增强教育。将该显微镜整合到核心显微镜设施中，将使本科生，研究生和博士后学员可以使用，并将由宾夕法尼亚州立大学提供常规成像研讨会。将开发针对K-12和本科教育者的新教学模块，这些教育者将开发大小科学和超分辨率显微镜的潜力。通过课程，包括Eberly科学学院，麦克奈尔学者，科学和工程研究妇女以及少数族裔本科研究经验的课程，将确保代表性不足的学生的访问和培训。公众对超分辨率显微镜及其优势的理解将被包括富兰克林研究所（科学博物馆）和宾夕法尼亚州立大学的AG Progress Days在内的多个外展活动和场地所促进，这些活动将使这项尖端的成像技术与成千上万的人相提并论。该显微镜实现的发现将推进对动植物开发，可持续农业和能源生产的研究，以及定义我们物理环境的化学相互作用。分辨率 - 分辨率的显微镜在生命科学中带来了巨大的新研究机会，并且在化学和材料研究方面具有新兴的能力。宾夕法尼亚州立大学将通过购买具有结构化照明显微镜（SIM）和随机光学重建显微镜（Storm）功能的超分辨率显微镜（SIM）的超分辨率显微镜来推动我们的基于光学成像的研究。两个成像模块都在单个显微镜平台上组合，并使用高磁化总内反射荧光（TIRF）目标，自动焦点保留系统和集成的多通道固态激光发射。对于宾夕法尼亚州立大学的生命科学显微镜和细胞仪设备（多用户核心设施）的设备，这种商业超分辨率显微镜的易用性是一个特别吸引人的功能。使用该显微镜的研究和培训将重点放在四个主要领域：（a）植物细胞生物学； （b）动物细胞生物学； （c）化学； （d）高级图像处理和模式识别。 （a） - （c）组的研究人员将利用该系统提供的分辨率提高，以将其研究推向迄今无法达到的精细生物学过程和分子相互作用的领域。同时，（d）组将与那些生成超分辨率成像数据的人合作，以提高我们从分子定位数据中识别结构和模式的能力。通过为样品标记和成像提供高度的灵活性，合并后的SIM/风暴显微镜将最大程度地使宾夕法尼亚州生命和化学科学研究人员受益最多。作为该项目不可或缺的组成部分，具有高级图像分析和模式识别的研究兴趣的教师将生成新的算法，用于分析SIM卡和风暴成像数据，这些数据将广泛适用于超分辨率显微镜，并将自由地传播到全球科学界。