MRI: Acquisition of a genomic sequencer within a shared resource facility for interdisciplinary sciences and training at Oklahoma State University

授予俄克拉荷马州州立大学（OSU）一个奖项，以获得一个Illumina NextSeq 500基因组测序仪。 测序技术对于包括所有学生水平的生物信息学在内的STEM课程至关重要。NextSeq将提供动手本科研究经验。 该工具将用于通过生物信息学证书研究生计划加强多样化，具有全球竞争力的STEM劳动力的发展，旨在提高研究生的学术和劳动力就业能力。该项目将加强跨学科用户的研究，包括生物化学和分子生物学，植物学，综合生物学，植物和土壤科学，动物科学，兽医科学，微生物学，植物和土壤科学，昆虫学，动物学等教师。该仪器将大大提高在宏基因组学研究中识别食品污染法医病原体的能力，包括通过国家微生物法医研究所食品和农业生物安全方案提高国家安全，该方案旨在快速识别与生物恐怖主义有关的病原体。 该项目将通过加强小麦、牛肉和许多其他农业重要作物和动物的遗传标记育种研究来造福社会。需要大量测序的现有跨学科项目，如小麦改良团队、俄克拉荷马州呼吸和传染病中心以及生物能源中心将从中受益。NextSeq 500是一种高通量测序仪，每次运行能够生成多达120千兆碱基对的序列数据。该仪器具有真核生物从头基因组测序、RNA-seq实验、宏基因组实验和其他现代高通量数据实验所需的能力，成本低，周转快。获得这一工具将加强研究，研究培训，合作，并在俄克拉荷马州州立大学多个跨学科项目的推广，包括农业育种，植物病毒生物多样性，植物生物恐怖主义法医学，与俄克拉荷马州的美洲原住民部落合作的秃鹰和金雕群体遗传学的现有国家领导计划，与区域机构的合作项目，包括兰斯顿大学（HBCU）的定向育种，与进化、气候、生态系统修复有关的环境宏基因组学项目，以及非模式生物的多个从头测序项目。本科生将通过收集和测序环境标本和学习相关的生物信息学课程和研讨会进行培训。该工具在战略上补充了现有的网络基础设施，用于OSU的数据管理，高吞吐量数据和大数据分析。

An award is made to Oklahoma State University (OSU) to acquire an Illumina NextSeq500 genomic sequencer. Sequencing technologies are essential to STEM curricula that include bioinformatics at all student levels. The NextSeq will provide hands-on undergraduate research experiences. The instrument will be used to enhance the development of a diverse, globally competitive STEM workforce through the Bioinformatics Certificate Graduate Program, designed to improve academic and workforce employability of graduate students. The project will enhance research from interdisciplinary users including faculty from Biochemistry and Molecular Biology, Botany, Integrative Biology, Plant and Soil Science, Animal Science, Veterinary Sciences, Microbiology, Plant and Soil Science, Entomology, Zoology, and many more. The instrument will dramatically improve capacity to identify pathogens in metagenomics studies for forensics of food contamination including improved national security through the National Institute for Microbial Forensics & Food and Agricultural Biosecurity program which seeks to rapidly identify pathogens related to bioterrorism. The project will benefit society by strengthening genetic marker breeding research in wheat, beef, and many other agriculturally important crops and animals. Existing interdisciplinary programs that require significant sequencing such as the Wheat Improvement Team, The Oklahoma Center for Respiratory and Infectious Disease, and the Bioenergy Center will benefit.The NextSeq500 is a high-throughput sequencing machine capable of generating up to 120 giga-base-pairs of sequence data per run. This instrument has the capacity needed for eukaryotic de novo genome sequencing, RNA-seq experiments, metagenomic experiments, and other modern high-throughput data experiments at low cost and rapid turnaround. Access to this instrument will strengthen research, research training, collaborations, and outreach across multiple interdisciplinary programs at Oklahoma State University, including existing national leadership programs in agricultural breeding, plant virus biodiversity, plant bioterrorism forensics, population genetics of bald and golden eagles in collaboration with Native American tribes in Oklahoma, collaborative projects with regional institutions including targeted breeding at Langston University (an HBCU), environmental metagenomics projects related to evolution, climate, ecosystem remediation, and multiple de novo sequencing projects of non-model organisms. Undergraduate students will be trained by collecting and sequencing environmental specimens and learning associated bioinformatics in classes and workshops. This instrument strategically complements existing cyberinfrastructure for data management, high throughput data, and big data analyses at OSU.