Collaborative Research: Do Cyanobacteria Drive Marine Hydrocarbon Biogeochemistry?

合作研究：蓝藻是否驱动海洋碳氢化合物生物地球化学？

• 批准号: 1635562
• 负责人: David Valentine
• 金额: $ 36.43万
• 依托单位: University of California-Santa Barbara
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-01 至 2020-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1635562&HistoricalAwards=false
• 关键词: Collaborative; Research; Do; Cyanobacteria; Drive

While the release of petroleum hydrocarbons into the ocean is recognized as an environmental and human hazard, a recent study has estimated that on an annual basis, the release of natural hydrocarbons by a single phytoplankton group (cyanobacteria) contributes at least ten times more total hydrocarbon to the surface ocean. This project will be the first in-depth study of the latent biogeochemical cycling of this huge pool of biogenic hydrocarbons. Using field studies, laboratory incubations of cyanobacteria, and state-of-the art chemical analysis, the researchers will examine the molecular structures, rates and mechanisms of production and removal, and the environmental conditions that control the cycling of this major pool of oceanic hydrocarbons. The results of this study will reveal significant new knowledge for improved understanding of a major carbon cycle in the ocean. Additionally, data could indicate a role for cyanobacterial hydrocarbons in preparing natural marine bacteria to respond to, and degrade petroleum spills, as well as a possible atmospheric impact (e.g. cloud formation) resulting from air-sea exchange of certain components of the hydrocarbon pool. This project will support undergraduate and graduate students, a postdoctoral investigator, and a new faculty member, and will engage participants from minority-serving institutions in California and North Carolina. Plans are also included to establish links with oil spill and biofuel researchers in order to evaluate additional practical applications for the data resulting from this study.The annual production of 308,000,000 - 771,000,000 tons of hydrocarbons by cyanobacteria has recently been reported and is a factor of 10 larger than marine petroleum hydrocarbon input from spills and natural seeps. Consequently, these biogenic hydrocarbons almost certainly have significant implications for the carbon cycle and the bacterial community composition in the ocean but have never been the subject of rigorous study. This project will investigate the distribution, partitioning, and cycling of biogenic hydrocarbons in the ocean, focusing on the abundance and molecular diversity of biogenic hydrocarbons in relation to cyanobacterial populations; the extent to which volatilization to the atmosphere acts as a sink for biogenic hydrocarbons; and the rate at which hydrocarbons are produced by cyanobacteria and consumed by hydrocarbon-degrading bacteria. Field studies across natural gradients in phytoplankton community structure and abundance will employ state of the art chemical analysis to evaluate the distribution of biogenic hydrocarbons, and together with incubation experiments will determine quantitative rates for biogenic hydrocarbons cycling in the surface ocean. Laboratory studies will augment field studies by assessing hydrocarbon production and loss mechanisms under carefully controlled laboratory conditions. Together, the project will obtain a quantitative understanding of this important component of the oceanic carbon cycle.

虽然石油碳氢化合物释放到海洋中被认为是对环境和人类的危害，但最近的一项研究估计，每年，单一浮游植物群（蓝藻）释放的天然碳氢化合物对表层海洋的碳氢化合物总量至少贡献十倍以上。该项目将是对这个巨大的生物碳氢化合物库的潜在生物地球化学循环的首次深入研究。研究人员将通过实地研究、蓝藻实验室孵化和最先进的化学分析，研究分子结构、产生和清除的速率和机制，以及控制这一主要海洋碳氢化合物库循环的环境条件。这项研究的结果将揭示重要的新知识，以增进对海洋主要碳循环的理解。此外，数据还可以表明蓝藻碳氢化合物在准备天然海洋细菌以响应和降解石油泄漏方面的作用，以及碳氢化合物池某些成分的海气交换可能产生的大气影响（例如云的形成）。该项目将为本科生和研究生、一名博士后研究员和一名新教员提供支持，并将吸引来自加利福尼亚州和北卡罗来纳州少数族裔服务机构的参与者。还包括与石油泄漏和生物燃料研究人员建立联系的计划，以评估本研究数据的其他实际应用。最近有报道称，蓝细菌每年生产 308,000,000 - 771,000,000 吨碳氢化合物，比泄漏和自然渗漏产生的海洋石油碳氢化合物输入量大 10 倍。 因此，这些生物碳氢化合物几乎肯定对海洋中的碳循环和细菌群落组成具有重大影响，但从未成为严格研究的主题。该项目将研究海洋中生物碳氢化合物的分布、分配和循环，重点关注与蓝藻种群相关的生物碳氢化合物的丰度和分子多样性；挥发到大气中作为生物碳氢化合物汇的程度；以及蓝细菌产生碳氢化合物和碳氢化合物降解细菌消耗碳氢化合物的速率。浮游植物群落结构和丰度自然梯度的实地研究将采用最先进的化学分析来评估生物碳氢化合物的分布，并与孵化实验一起确定表层海洋中生物碳氢化合物循环的定量速率。实验室研究将通过在严格控制的实验室条件下评估碳氢化合物的产生和损失机制来增强现场研究。 该项目将共同获得对海洋碳循环这一重要组成部分的定量了解。

项目成果

The Wax–Liquid Transition Modulates Hydrocarbon Respiration Rates in Alcanivorax borkumensis SK2

蜡-液体转变调节 Alcanivorax borkumensis SK2 的碳氢化合物呼吸速率

• DOI: 10.1021/acs.estlett.8b00143
• 发表时间: 2018
• 期刊: Environmental Science & Technology Letters
• 影响因子: 10.9
• 作者: Lyu, Li-Na;Ding, Haibing;Cui, Zhisong;Valentine, David L.
• 通讯作者: Valentine, David L.

Examining Inputs of Biogenic and Oil-Derived Hydrocarbons in Surface Waters Following the Deepwater Horizon Oil Spill

检查深水地平线漏油后地表水中生物和石油衍生碳氢化合物的输入

• DOI: 10.1021/acsearthspacechem.9b00090
• 发表时间: 2019
• 期刊: ACS Earth and Space Chemistry
• 影响因子: 3.4
• 作者: White, Helen K.;Marx, Charles T.;Valentine, David L.;Sharpless, Charles;Aeppli, Christoph;Gosselin, Kelsey M.;Kivenson, Veronika;Liu, Rachel M.;Nelson, Robert K.;Sylva, Sean P.
• 通讯作者: Sylva, Sean P.

Microbial production and consumption of hydrocarbons in the global ocean

• DOI: 10.1038/s41564-020-00859-8
• 发表时间: 2021-02-01
• 期刊: NATURE MICROBIOLOGY
• 影响因子: 28.3
• 作者: Love, Connor R.;Arrington, Eleanor C.;Valentine, David L.
• 通讯作者: Valentine, David L.