IOS EDGE: Functional-genomics tools for Cnidarian-dinoflagellate symbiosis

IOS EDGE：用于刺胞动物-甲藻共生的功能基因组学工具

• 批准号: 1645164
• 负责人: Virginia Weis
• 金额: $ 225万
• 依托单位: Oregon State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-05-15 至 2023-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1645164&HistoricalAwards=false
• 关键词: IOS; EDGE; Functional; genomics; tools

Coral reefs are profoundly important, diverse ecosystems that are threatened worldwide by environmental variation and stress. They have huge economic value as fisheries, storm barriers and destinations for ecotourism. Corals are made up of large numbers of interconnected animal hosts, called polyps that house microscopic algae inside their cells. This partnership, or symbiosis, is the foundation of the entire coral reef ecosystem. The polyps receive photosynthetic nutrients from the algae, and the algae receive nutrients from the polyps. While a great deal of attention has been focused on the environmental threats to corals, there remains only a partial understanding of the microscale cellular, molecular, and genetic mechanisms that underpin the coral-algal symbiosis. Insight into regulation of this symbiosis will provide a stronger foundation for studies of coral health and coral stress, such as coral bleaching in which the host polyps lose their symbiotic algae. This project will bring together a diverse team of coral biologists, cell biologists and geneticists to study a small sea anemone that serves as an excellent proxy for corals, which do not survive well in the lab, are slow growing, and difficult to collect because of their endangered status. In contrast, the fast-growing, weedy sea anemone Aiptasia will enable the researchers to make rapid progress on the study of coral symbiosis. This award is focused on technique development and swift dissemination of results through online communication platforms to both the scientific community and the public. A variety of genetic techniques will be developed, including gene editing in both partners to be able to test hypotheses about the involvement of specific genes in coral health and stress. To increase the effectiveness and efficiency of research efforts to understand the cellular, molecular and genetic foundations of this symbiosis, the researchers will develop and optimize approaches to rear the complete life cycle of Aiptasia in the laboratory. This award will contribute to the training of scientists and expose school-aged children and the general public to coral reef and symbiosis science.The ecological, economic, and aesthetic importance of coral reefs is widely recognized, as are the threats they face from environmental stress. Nonetheless, the underlying molecular and cell biology of corals and their resident endosymbiotic dinoflagellates (genus Symbiodinium) that are critical for host nutrition remain poorly understood. A major reason for this paucity of knowledge is the lack of methods for mutant isolation and analysis, gene knockdown, gene knockout, and gene tagging. Without these, even powerful genomic and transcriptomic analysis largely yield correlations whose implications for causal mechanisms remain uncertain. This project focuses on development of approaches to test gene function in a rapidly developing model system, the sea anemone Aiptasia and the Symbiodinium minutum (Clade B1) strain SSB01, which can form a stable endosymbiosis with Aiptasia, and can grow well in culture. Genetic manipulation in SSB01 and in small polyps will include morpholino and CRISPR/Cas9 approaches to achieve gene knockdown, knockout and gene tagging. The researchers will identify and test compounds from natural substrates and biofilms to achieve larval settlement, and will identify and test anemone neuropeptides as a means to achieve metamorphosis of Aiptasia routinely in the laboratory. Finally, a set of interwoven communications platforms and activities will be developed to rapidly disseminate information gained in the project to the broader scientific community.

珊瑚礁是极其重要的、多样化的生态系统，在世界范围内受到环境变化和压力的威胁。它们作为渔业、风暴屏障和生态旅游目的地具有巨大的经济价值。 珊瑚是由大量相互联系的动物宿主组成的，这些动物宿主被称为珊瑚虫，它们的细胞内有微小的藻类。 这种伙伴关系或共生关系是整个珊瑚礁生态系统的基础。珊瑚虫从藻类那里获得光合作用的营养，而藻类从珊瑚虫那里获得营养。 虽然大量的注意力都集中在珊瑚的环境威胁，仍然只有部分的理解的微观细胞，分子和遗传机制，支持珊瑚藻共生。深入了解这种共生关系的调节将为珊瑚健康和珊瑚压力的研究提供更坚实的基础，例如珊瑚漂白，其中宿主水螅失去了共生藻类。 该项目将汇集一个由珊瑚生物学家，细胞生物学家和遗传学家组成的多元化团队，研究一种小型海葵，这种海葵是珊瑚的极好替代品，珊瑚在实验室中无法很好地生存，生长缓慢，并且由于其濒危状态而难以收集。 相比之下，快速生长的杂草海葵Aiptasia将使研究人员能够在珊瑚共生研究方面取得快速进展。 该奖项侧重于技术开发和通过在线交流平台向科学界和公众迅速传播成果。 将开发各种遗传技术，包括双方的基因编辑，以便能够测试有关特定基因参与珊瑚健康和压力的假设。 为了提高研究工作的有效性和效率，以了解这种共生的细胞，分子和遗传基础，研究人员将开发和优化方法，以在实验室中培养Aiptasia的完整生命周期。 该奖项将有助于科学家的培训，并使学龄儿童和公众了解珊瑚礁和共生科学。珊瑚礁的生态、经济和美学重要性以及它们面临的环境压力威胁得到了广泛的认可。尽管如此，珊瑚和其居民的内共生甲藻（属共生藻），是宿主营养的关键的基本分子和细胞生物学仍然知之甚少。这种知识匮乏的一个主要原因是缺乏突变体分离和分析、基因敲除、基因敲除和基因标记的方法。如果没有这些，即使是强大的基因组和转录组学分析在很大程度上产生的相关性，其因果机制的影响仍然不确定。该项目的重点是在一个快速发展的模型系统中开发测试基因功能的方法，该模型系统是海葵Aiptasia和Symbiodinium minutum（分支B1）菌株SSB 01，该菌株可以与Aiptasia形成稳定的内共生关系，并且可以在培养物中生长良好。SSB 01和小息肉中的基因操作将包括morpholino和CRISPR/Cas9方法来实现基因敲除、敲除和基因标记。 研究人员将从天然基质和生物膜中识别和测试化合物以实现幼虫定居，并将识别和测试海葵神经肽作为在实验室中常规实现Aiptasia变态的手段。最后，将开发一套相互交织的交流平台和活动，以便向更广泛的科学界迅速传播项目中获得的信息。

项目成果

Symbiont population control by host-symbiont metabolic interaction in Symbiodiniaceae-cnidarian associations

• DOI: 10.1038/s41467-019-13963-z
• 发表时间: 2020-01
• 期刊: Nature Communications
• 影响因子: 16.6
• 作者: T. Xiang;E. Lehnert;Robert E. Jinkerson;S. Clowez;Rick G. Kim;Jan C. DeNofrio;J. Pringle;A. Grossman-A.-Gr

Impact of Menthol on Growth and Photosynthetic Function of Breviolum Minutum (Dinoflagellata, Dinophyceae, Symbiodiniaceae) and Interactions with its Aiptasia Host

薄荷醇对短叶藻（甲藻、甲藻纲、共生藻科）生长和光合功能的影响及其与其寄主的相互作用

• DOI: 10.1111/jpy.13081
• 发表时间: 2021
• 期刊: Journal of Phycology
• 影响因子: 2.9
• 作者: Clowez, Sophie;Renicke, Christian;Pringle, John R.;Grossman, Arthur R.
• 通讯作者: Grossman, Arthur R.

Insights into coral bleaching under heat stress from analysis of gene expression in a sea anemone model system

• DOI: 10.1073/pnas.2015737117
• 发表时间: 2020-11-17
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Cleves, Phillip A.;Krediet, Cory J.;Pringle, John R.
• 通讯作者: Pringle, John R.

Cnidarian-Symbiodiniaceae symbiosis establishment is independent of photosynthesis

• DOI: 10.1016/j.cub.2022.04.021
• 发表时间: 2022-06-06
• 期刊: CURRENT BIOLOGY
• 影响因子: 9.2
• 作者: Jinkerson, Robert E.;Russo, Joseph A.;Xiang, Tingting
• 通讯作者: Xiang, Tingting