Collaborative Research: EDGE FGT: Development of genetic and functional genomics resources for Ipomoea (morning glory)

合作研究：EDGE FGT：Ipomoea（牵牛花）遗传和功能基因组学资源的开发

• 批准号: 2220214
• 负责人: Regina Baucom
• 金额: $ 109.51万
• 依托单位: Regents of the University of Michigan - Ann Arbor
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-01 至 2026-08-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2220214&HistoricalAwards=false
• 关键词: Collaborative; Research; EDGE; FGT; Development

Understanding how plants adapt to their environment has become more important than ever in a time of climate change. For many decades, morning glories have been the species of choice for studying fundamental questions in evolutionary biology, particularly for weedy morning glories, ornamentals, and the globally important food crop, sweetpotato. Such evolutionary studies can be aided through the application of modern molecular and genomic tools; yet, while morning glories are a captivating and economically important group, resources for linking genetics and variation in form are lacking. This project combines state-of-the-art approaches to discover how changes in DNA influence plant form and ecological function including responses to environmental change. The development of these approaches in model morning glory species will broaden the scope of current evolutionary investigations. Further, the dissemination of newly developed genetic resources and research protocols will assist the larger research community studying the molecular basis of variation in plant traits and adaptations. To reach a broad audience, including plant scientists and undergraduate students, YouTube videos will be developed as will methods designed for undergraduate laboratory courses in plant evolution, genetics, and genomics. Additionally, student artists will capture variation in morning glory traits from a garden display held at the Matthaei Botanical Garden, and these artistic renditions will be displayed at the Natural History Museum in Ann Arbor, Michigan, thus reaching the public via multiple avenues.Novel transformation techniques using multiple Agrobacterium strains and the development of mutant lines via the use of fast neutrons (FN) have expanded transformation capabilities to a wider group of plants and have likewise allowed for the relatively rapid development of random mutations for understanding gene function. This project will adapt these methods to morning glory species (Ipomoea) that have been used in ecological and evolutionary genetics applications (I. purpurea, I. hederacea, I. lacunosa), species that are close relatives of the important crop sweetpotato (I. trifida and I. lacunosa), or phylogenetically useful outgroups (I. obscura). Genetic mapping populations for I. lacunosa and I. purpurea will be generated, genotyped, and disseminated for use by scientists that seek to interrogate a number of evolutionary and applied questions. Gene expression profiles for these populations will likewise be generated. Education of the plant science community in transformation protocols and the distribution of resources are significant foci of this work. An Ipomoea Wiki will be developed to integrate and disseminate protocols, videos, and sequence data links to allow researchers and educators to reconstruct the process of genetic transformation and to easily locate project data. Sequences will be deposited to public databases as generated, following initial cleaning steps. This project will support 1) training opportunities for undergraduate researchers along with members of the plant science community including graduate students, postdoctoral researchers, and professors, 2) public displays and artistic renditions that capture the remarkable and memorable variation found within and between species belonging to the Ipomoea genus.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

在气候变化的时代，了解植物如何适应环境变得比以往任何时候都更加重要。几十年来，牵牛花一直是研究进化生物学基本问题的首选物种，特别是对于杂草牵牛花，植物和全球重要的粮食作物甘薯。这种进化研究可以通过现代分子和基因组工具的应用来帮助;然而，虽然牵牛花是一个迷人的和经济上重要的群体，但缺乏将遗传学和形式变异联系起来的资源。该项目结合了最先进的方法来发现DNA的变化如何影响植物的形态和生态功能，包括对环境变化的反应。这些方法在模式牵牛花物种中的发展将拓宽当前进化研究的范围。此外，传播新开发的遗传资源和研究规程将有助于更大的研究界研究植物性状和适应性变异的分子基础。为了接触到包括植物科学家和本科生在内的广泛受众，将开发YouTube视频，以及为植物进化，遗传学和基因组学的本科实验室课程设计的方法。此外，学生艺术家将捕捉牵牛花性状的变化，从一个花园显示在Matthaei植物园举行，这些艺术再现将显示在自然历史博物馆在安阿伯，密歇根州，使用多种农杆菌菌株的新型转化技术和通过使用快中子（FN）开发突变株系已经将转化能力扩展到更广泛的植物群体，并且同样允许相对快速地开发随机突变以理解基因功能。这个项目将使这些方法适用于已经在生态学和进化遗传学应用中使用的牵牛花物种（Ipomoea）。普尔普雷亚岛常春藤岛lacunosa）、重要作物甘薯（I. Trifida和我lacunosa），或遗传学上有用的外群（I. obscura）。遗传作图群体I. lacunosa和I. purpurea将被生成，基因分型，并传播给科学家使用，寻求询问一些进化和应用问题。同样将生成这些群体的基因表达谱。植物科学界在转化方案和资源分配方面的教育是这项工作的重要重点。将开发一个Ipomoea Wiki，以整合和传播协议、视频和序列数据链接，使研究人员和教育工作者能够重建遗传转化过程，并轻松定位项目数据。在初始清理步骤后，将生成的序列存入公共数据库。该项目将支持1）本科研究人员沿着植物科学界成员（包括研究生、博士后研究人员和教授）的培训机会，（二）公开展示和艺术表演，捕捉属于Ipomoea属的物种内和物种之间发现的显着和令人难忘的变化。该奖项反映了NSF的法定使命，并已被认为值得通过使用评估支持基金会的学术价值和更广泛的影响审查标准。