The role of WIH1/2 peptides in the transition from somatic to sporogenic fate in plants

WIH1/2 肽在植物从体细胞到孢子发生命运转变中的作用

• 批准号: 226768757
• 负责人: Professor Dr. Thomas Laux
• 金额: --
• 依托单位: Institut für Biologie III
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2012
• 资助国家: 德国
• 起止时间: 2011-12-31 至 2022-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/226768757?language=en
• 关键词: role; WIH1; peptides; transition; somatic

The goal of this project is to investigate the molecular mechanisms that regulate germline initiation in plants. Unlike animals where the germline is set apart from the soma early in embryogenesis, plants have evolved the fascinating ability to generate haploid gametes from somatic cells at the adult stage; a property that reflects the remarkable developmental plasticity of plant cells and that is of economic importance for seed production and plant propagation. In the female organs of the flower, a single somatic cell of each ovule becomes reprogrammed into a megaspore mother cell (MMC), which subsequently enters meiosis from which ultimately the egg cell is derived. The molecular pathways regulating the establishment of the MMC are largely unknown. During the first funding period, we discovered a novel molecular mechanism for the plant specific WIH1 (WINDHOSE1) and WIH2 peptides in initiating MMC development. Our data suggest a model where WIH peptides in the epidermis of the ovule act as novel tether molecules for RABA1-decorated vesicles that deliver the auxin transport protein PINFORMED1 to the plasma membrane. Subsequent accumulation of the phytohormone auxin in the epidermis is essential for the development of the underlying MMC. In the second funding period, we aim at testing this model and dissect the molecular principles of MMC regulation by WIH function. These studies will enhance our understanding of fundamental developmental processes that reprogram cell fate and initiate generative pathways, and at the same time contribute to concepts of reproductive strategies in economically important plant species.

本项目的目的是研究调节植物种系起始的分子机制。与动物不同的是，在胚胎发生的早期，种系与体细胞是分开的，植物在成年阶段已经进化出了从体细胞产生单倍体配子的迷人能力；这一特性反映了植物细胞显著的发育可塑性，对种子生产和植物繁殖具有重要的经济意义。在花的雌性器官中，每个胚珠的单个体细胞被重新编程为大孢子母细胞（MMC），随后进入减数分裂，最终产生卵细胞。调控MMC建立的分子途径在很大程度上是未知的。在第一个资助期内，我们发现了植物特异性wi1 （WINDHOSE1）和WIH2肽启动MMC发展的新分子机制。我们的数据表明，在一个模型中，胚珠表皮中的with肽作为raba1修饰的囊泡的新型系绳分子，将生长素运输蛋白pinformmed1传递到质膜。随后表皮中植物激素生长素的积累对潜在MMC的发展至关重要。在第二个资助期，我们的目标是测试这个模型，并剖析通过WIH功能调控MMC的分子原理。这些研究将增强我们对细胞命运重编程和启动生殖途径的基本发育过程的理解，同时有助于了解重要经济植物物种的生殖策略。