EAGER: Integrating Multiple Biomarkers in Nano-Sensor Arrays for Enhanced Detection of Prostate Cancer

EAGER：将多种生物标记物集成到纳米传感器阵列中以增强前列腺癌的检测

• 批准号: 1649934
• 负责人: Frances Williams
• 金额: $ 30万
• 依托单位: Tennessee State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-15 至 2018-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1649934&HistoricalAwards=false
• 关键词: EAGER; Integrating; Multiple; Biomarkers; Nano

The National Science Foundation uses the Early-concept Grants for Exploratory Research (EAGER) funding mechanism to support exploratory work in its early stages on untested, but potentially transformative, research ideas or approaches. This EAGER project was awarded as a result of the invitation in the Dear Colleague Letter NSF 16-080 to proposers from Historically Black Colleges and Universities to submit proposals that would strengthen research capacity of faculty at the institution. The project at Tennessee State University aims to design and develop nano-cantilever resonators functionalized with multiple biomarkers to provide for an accurate, highly sensitive, fast, and low-cost method for the detection of prostate cancer.This project seeks to explore the feasibility of integrating multiple biomarkers in a nano-cantilever array to provide for an accurate, highly sensitive, fast, and low-cost method for the detection of prostate cancer. The sensor cantilever array will be designed and fabricated, each cantilever will be functionalized with a different biomarker, and the response from the array will be measured and mapped together to provide a model for detecting prostate cancer. It is proposed that the sensor array will generate more data about the sample to be analyzed and provide for a more accurate and specific detection method, reducing the number of false positives generated. The main tasks to be completed to achieve this objective are: the design of the nano-cantilevers; fabrication of the nano-electromechanical systems devices; functionalizing the nano-resonator surfaces, including the conjugation of the gold nanoparticles with each of the four biomarkers to be used and the application of each bio-substrate on a nano-cantilever; and testing of the nano-cantilever array. This work could be expanded in the future to provide a framework for the use of multiple biomarkers for other sensing mechanisms where specificity and sensitivity are an issue. This EAGER project is funded by the Engineering Directorate.

美国国家科学基金会使用探索性研究早期概念赠款（EAGER）资助机制，以支持未经测试但可能具有变革性的研究想法或方法的早期阶段的探索性工作。EAGER项目是由于在亲爱的同事信NSF 16-080中邀请来自历史上黑人学院和大学的提议者提交提案，以加强该机构教师的研究能力而获得的。田纳西州立大学的该项目旨在设计和开发具有多种生物标志物功能化的纳米悬臂梁谐振器，以提供用于检测前列腺癌的准确、高灵敏度、快速和低成本的方法。该项目旨在探索在纳米悬臂梁阵列中整合多种生物标志物的可行性，以提供准确、高灵敏度、快速、低成本的前列腺癌检测方法。传感器悬臂阵列将被设计和制造，每个悬臂将被不同的生物标志物功能化，并且来自阵列的响应将被测量和映射在一起，以提供用于检测前列腺癌的模型。建议传感器阵列将生成更多关于待分析样品的数据，并提供更准确和更具体的检测方法，减少生成的假阳性的数量。 实现这一目标的主要任务是：纳米悬臂梁的设计;纳米机电系统设备的制造;纳米谐振器表面的功能化，包括金纳米颗粒与四种生物标志物的结合，以及在纳米悬臂梁上应用每种生物基质;以及纳米悬臂梁阵列的测试。 这项工作可以在未来扩大，为使用多种生物标志物的其他传感机制提供一个框架，其中特异性和灵敏度是一个问题。EAGER项目由工程局资助。