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Virus trafficking in insect midgut cells

Virus trafficking in insect midgut cells
昆虫中肠细胞中的病毒贩运
批准号:
1653021
负责人:
Gary Blissard
金额:
$52.72万
依托单位国家:
美国
项目类别:
Standard Grant
财政年份:
2017
资助国家:
美国
项目状态:
已结题
起止时间:
2017-07-01 至 2021-06-30

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中文摘要
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英文摘要
Arthropods represent the largest animal biomass on earth. Their populations are regulated by many viral diseases, and arthropods also vector many viruses that cause important diseases of plants and animals. Thus, interactions between arthropods and viruses in nature are ubiquitous and important. In most insect-virus interactions, a virus enters the insect by the oral route and must penetrate the cells of the insect's gut in order to have access to the other cells and tissues of the insect. Although it is a critical step in the biological interaction, very little is known about how viruses move through insect midgut cells. The proposed studies will examine those interactions, focusing on the mechanisms of directional (polarized) viral transit through the epithelial cells of the insect midgut. For these studies, a combination of cultured cells and transgenic Drosophila will be used, and combined with a variety of genetic and molecular approaches, to discover the cellular proteins that interact with and direct viral proteins across the insect gut cells. Broader impacts of these studies of virus-insect interactions in the insect midgut will include applications in diverse areas such as agricultural crop protection, forestry, animal science, and human medicine. Understanding the specific viral signals and interacting midgut cell proteins and pathways that regulate or restrict virus trafficking through midgut cells can be used to both enhance infection by beneficial viruses (such as biocontrol agents in agriculture) and to inhibit viral transmission in insect vectors of plant and animal diseases. Many plant and animal viruses are vectored by insects, and insects are also infected by a number of pathogenic viruses. Typically, after the virus is consumed orally, the virus enters and replicates in polarized epithelial cells of the insect midgut. Polarized trafficking of viral components delivers the virus particle or components for assembly, to the basal membranes of the midgut cell where the virus may assemble and exit the cell by budding. Virus transport across insect midgut epithelial cells is not well understood even in the best characterized systems, and little is known about the mechanistic nature of polarized protein transport in the insect gut, due to the difficult nature of experimental manipulation in this tissue. This project will utilize an innovative experimental design that leverages two powerful model systems for identifying specific proteins, pathways, and signals involved in virus-midgut trafficking. Using ectopic expression of viral proteins in Drosophila midgut cells followed by RNAi screens using libraries of RNAi fly strains, and high throughput analysis of a Drosophila cell line, entire candidate pathways will be screened to identify specific proteins and pathways necessary for polarized trafficking of two model viral proteins in the midgut. To identify protein signals necessary for midgut trafficking, an extensively studied viral protein (baculovirus GP64) will be analyzed in a natural host insect, the lepidopteran Trichoplusia ni. The identification of viral protein signals and midgut proteins and pathways that mediate and regulate targeted trafficking will generate new paradigms for understanding pathogen-insect interactions.
期刊论文(11)
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会议论文
DOI: 10.1128/jvi.00353-19
发表时间: 2019-05
期刊: Journal of Virology
影响因子: 5.4
作者: [A. Shrestha;Kan Bao;Wenbo Chen;Ping Wang;Z. Fei;G. Blissard]
通讯作者: A. Shrestha;Kan Bao;Wenbo Chen;Ping Wang;Z. Fei;G. Blissard
Efficient entry of budded virions of Autographa californica multiple nucleopolyhedrovirus into Spodoptera frugiperda cells is dependent on dynamin, Rab5, and Rab11
苜蓿银纹夜蛾多核多角体病毒的出芽病毒粒子有效进入草地贪夜蛾细胞取决于动力、Rab5 和 Rab11
DOI: 10.1016/j.ibmb.2020.103409
发表时间: 2020
期刊: Insect Biochemistry and Molecular Biology
影响因子: 3.8
作者: [Yue Qi, Li Jingfeng, Guo Ya, Yan Fanye, Liu Ximeng, Blissard Gary, Li Zhaofei]
通讯作者: Li Zhaofei
DOI: 10.1146/annurev-virology-092917-043356
发表时间: 2018-01-01
期刊: ANNUAL REVIEW OF VIROLOGY, VOL 5
影响因子: --
作者: [Blissard, Gary W., Theilmann, David A.]
通讯作者: Theilmann, David A.
Distinct Roles of Cellular ESCRT-I and ESCRT-III Proteins in Efficient Entry and Egress of Budded Virions of Autographa californica Multiple Nucleopolyhedrovirus
细胞 ESCRT-I 和 ESCRT-III 蛋白在苜蓿银纹夜蛾多重核多角体病毒出芽病毒粒子有效进入和排出中的不同作用
DOI: 10.1128/jvi.01636-17
发表时间: 2017-10
期刊: Journal of Virology
影响因子: 5.4
作者: [Yue Qi, Yu Qianlong, Yang Qi, Xu Ye, Guo Ya, Blissard Gary, Li Zhaofei]
通讯作者: Li Zhaofei
9
    Mechanisms of viral protein trafficking in polarized insect cells
    Virus trafficking in insect midgut cells
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