CAREER: Modeling dorsal root ganglia: electrophysiology of microelectrode recording and stimulation

职业：背根神经节建模：微电极记录和刺激的电生理学

• 批准号: 1653080
• 负责人: Timothy Bruns
• 金额: $ 54.95万
• 依托单位: Regents of the University of Michigan - Ann Arbor
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-07-01 至 2022-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1653080&HistoricalAwards=false
• 关键词: CAREER; Modeling; dorsal; root; ganglia

PI: Bruns, TimothyProposal #: 1653080The goal of this project is to study and develop a computer model for dorsal root ganglia (DRG). DRG are part of the nervous system next to the spinal cord. Recently, DRG are being looked at as a place to stimulate to help people with different medical conditions such as chronic pain, spinal cord injury, and amputation. Current electrodes that stimulate DRG can be improved. This would allow for increases in patient care. We believe that a greater understanding of DRG function will help lead to electrode improvements. Two main research studies will be performed. First, small electrodes will be used in and near the DRG in animal studies. These studies will improve understanding of the signals made by DRG nerves. Second, the cell types in DRG will be studied and a computer model of the DRG will be created and validated. This model will improve our understanding of how the DRG functions and allow for the development of treatments that can be directed towards the DRG, such as to improve pain control or impact bowel and bladder function in individuals with paralysis. Outreach activities are planned that will expose middle school through college students to the exciting world of bioelectricity. Workshops will be held for local youths on how the nervous system works. In addition, a computer game will be created that teaches the basics of the nervous system.The objective of this project is to electrophysiologically and anatomically characterize end model dorsal root ganglia (DRG). DRG are unique nervous system structures where cell bodies for sensory neurons are concentrated next to the spinal cord. DRG have become an attractive location for therapeutic intervention, with applications ranging from chronic pain modulation to bladder control after spinal cord injury; however current electrode interfaces with DRG for clinical care and preclinical studies lack efficacy. While individual DRG neurons have been well characterized and utilized in in vitro neuroscience applications, the arrangement and electrical characteristics f in vivo whole DRG is not understood. This project will measure in vivo electrophysiology characterization of DRG-electrode interactionsas well as developing an in silico prediction of DRG-electrode interactions. The model will provide the ability to predict partial- and whole-DRG activation and neural signals for electrical and sensory stimuli. The educational objective of this proposal is to develop new and expand upon existing bioelectricity educational materials within and outside the University of Michigan by focusing on students from middle school through university level. In addition, a mobile game will be developed that can be used to interest and education K12 students as well as the general public about neuroscience.

主要研究者：Bruns，Alfrethy提案编号：1653080本项目的目标是研究和开发背根神经节（DRG）的计算机模型。DRG是神经系统的一部分，靠近脊髓。最近，DRG被视为一个刺激的地方，以帮助患有不同疾病的人，如慢性疼痛，脊髓损伤和截肢。可以改进刺激DRG的电流电极。这将增加对病人的护理。我们相信，对DRG功能的更深入了解将有助于电极的改进。 将进行两项主要研究。首先，在动物研究中，将在DRG内和附近使用小电极。这些研究将提高对DRG神经信号的理解。其次，将研究DRG中的细胞类型，并创建和验证DRG的计算机模型。 该模型将提高我们对DRG功能的理解，并允许开发可针对DRG的治疗方法，例如改善疼痛控制或影响瘫痪患者的肠道和膀胱功能。 外展活动的计划，将暴露从中学到大学的学生到生物电的令人兴奋的世界。将为当地年轻人举办关于神经系统如何工作的讲习班。此外，还将制作一个电脑游戏，教授神经系统的基础知识。本项目的目标是从电生理学和解剖学上表征端模型背根神经节（DRG）。背根神经节是独特的神经系统结构，其中感觉神经元的细胞体集中在脊髓旁边。DRG已成为治疗干预的有吸引力的位置，其应用范围从慢性疼痛调节到脊髓损伤后的膀胱控制;然而，用于临床护理和临床前研究的与DRG的当前电极接口缺乏功效。虽然单个DRG神经元已被很好地表征并用于体外神经科学应用，但体内整个DRG的排列和电特性尚不清楚。该项目将测量DRG电极相互作用的体内电生理学特征，并开发DRG电极相互作用的计算机预测。 该模型将提供预测部分和整个DRG激活以及电和感觉刺激的神经信号的能力。 该提案的教育目标是通过关注中学到大学的学生，在密歇根大学内外开发新的和扩展现有的生物电教育材料。 此外，还将开发一款移动的游戏，用于吸引和教育K12学生以及公众对神经科学的认识。

项目成果

Dorsal root ganglion stimulation for chronic pain modulates Aβ-fiber activity but not C-fiber activity: A computational modeling study

• DOI: 10.1016/j.clinph.2019.02.016
• 发表时间: 2019-06-01
• 期刊: CLINICAL NEUROPHYSIOLOGY
• 影响因子: 4.7
• 作者: Graham, Robert D.;Bruns, Tim M.;Lempka, Scott F.
• 通讯作者: Lempka, Scott F.

High-density neural recordings from feline sacral dorsal root ganglia with thin-film array

使用薄膜阵列对猫骶骨背根神经节进行高密度神经记录

• DOI: 10.1088/1741-2552/abe398
• 发表时间: 2021
• 期刊: Journal of Neural Engineering
• 影响因子: 4
• 作者: Sperry, Zachariah J;Na, Kyounghwan;Jun, James;Madden, Lauren R;Socha, Alec;Yoon, Eusik;Seymour, John P;Bruns, Tim M
• 通讯作者: Bruns, Tim M

The Effect of Clinically Controllable Factors on Neural Activation During Dorsal Root Ganglion Stimulation

• DOI: 10.1111/ner.13211
• 发表时间: 2020-06-24
• 期刊: NEUROMODULATION
• 影响因子: 2.8
• 作者: Graham, Robert D.;Bruns, Tim M.;Lempka, Scott F.
• 通讯作者: Lempka, Scott F.

Spatial models of cell distribution in human lumbar dorsal root ganglia.

人腰椎背根神经节细胞分布的空间模型。

• DOI: 10.1002/cne.24848
• 发表时间: 2020
• 期刊: The Journal of comparative neurology
• 作者: Sperry,ZachariahJ;Graham,RobertD;Peck-Dimit,Nicholas;Lempka,ScottF;Bruns,TimM
• 通讯作者: Bruns,TimM